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36218ae2_Flashcards_about_MAPK__Question
[Answer] It is used when you need to specifically detect one protein in a mix of proteins. [Question]
Why use Western Blotting?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] electrophoretically [Question]
How are the proteins transfered from the polyacrylimide gel to NC membrane?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The buffer, the membrane, voltage, time and charge. [Question]
Factors that influence protein transfer
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] If the current is too high, low MW proteins could migrate too fast and not get a chance to bind to the membrane. [Question]
Why is voltage important when transferring a protein from polyacrylimide gel to a hydrophobic membrane like NC or PVDF?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It consists of TBST+5%BSA. It's purpose is to bind to unoccupied sites on the NC filter so antibodies won't stick non-specifically. [Question]
What does the blocking buffer consist of and what is it's purpose?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Tris-Buffered Saline and Tween. [Question]
What does TBST stand for?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Reduces non-specific binding of proteins to the antigens. [Question]
What does Tween do?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The substances that antibodies bind to. [Question]
antigen
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Is a tetramer composed of 2 heavy chains and 2 light chains that are held together by disulfide bridges. [Question]
IgG
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The AA sequence in the base of the "Y". Does not vary for a given species. [Question]
Constant Region
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The AA sequence at the ends of the "Y". Does very and is the part of the IgG that bind to antigen. The diff AA seq. of the var. reg's in diff. IgG mol'cs creates spec. binding sites that bind to diff antigens. [Question]
Variable Region
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Ab's raised against Ab's are secondary Ab's. Ex. using a goat to raise Ab's agains rabbit IgG's. Sec. Ab's bind to the constant region of primary antibodies. [Question]
What does it mean to raise antibodies against antibodies?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] to the protein of interest. [Question]
The primary antibody is what binds to the...
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] visualize the bound primary protein by producing a signal b/c it usually has a tag or peroxidase enzyme covalently linked to the con. reg. [Question]
The secondary allows you to...
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] An animal is injected with the cons. reg. portion of IgG from the animal in which the primary Ab is raised. [Question]
How is the secondary Ab created?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] They can be used to help determine where in a cell an antigen may be concentrated. Whether a cell express a certain mol'c on it's surface, whether a mol'c is present in a sample, or to quantify the amount of antigen in sample and for purifying an antigen [Question]
How are Ab's helpful in biochem?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Yes, some mol'cs have sim. structures. This is called cross reacting. [Question]
Is it possible for an antibody to bind an antigen other than the one for which it was created?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Enzyme Linked Immuno Sorbent Assay [Question]
ELISA stands for
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It is used to detect antigen-antibody complex with the help of an enzyme that converts a substrate to a colored product. [Question]
What does an ELISA accomplish?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] inositol monophosphate, which is the breakdown product of a critical second messenger that is produced immediately after sperm bind (before Ca influx) [Question]
What antigen is detected by ELISA?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] antibody capture assay, two-site capture assay and competition assay. [Question]
What are the three types of ELISA tests?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The activation of PLC and the subsequent production of IP3. [Question]
What is the trigger for the cytoplasmic Ca influx in fertilized sea urchin eggs?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It is a lipase that hydrolysis reactions on lipids to cut them into smaller pieces. [Question]
What is PLC?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It catalyzes the cleavage of the phosphorylated head group from a phospholipid called PIP2. [Question]
What does PLC do?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] In the inner leaflet of the PM. [Question]
Where is PIP2 located?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Large quantities of IP3 and DAG. [Question]
When PLC cleaves the IP3 head from PIP2, what is produced?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It diffuses throughout the cytoplasm where it interacts with specific receptors. Receptor binding on the ER trigger Ca channels to open producing the Ca influx. [Question]
What happens to IP3 when it is cleaved?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] To prevent IP1 from being broken down into free inositol. [Question]
Why is LiCl added to the ELISA samples?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] antigen->primary Ab->secondary Ab+covalently linked peroxidase or alkaline phosphate-> enzyme substrate(TMB) [Question]
What is the binding order in antibody capture assay?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Ab #1->antigen/sample->Ab #2-peroxidase conjugate->TMB [Question]
What is the binding order in two site capture assay?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] secondary Ab(anti-serum agst mouse IgG)->IP1-coupled w/peroxidase conjugate followed by monoclonal Ab against IP1(Primary Ab)->TMB [Question]
What is the binding order for competition assay?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The IP1 conjugate competes with the IP1 present in egg lysate samples to bind to a fixed # of primary Ab's present in well [Question]
How does the competition assay work?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It is used to produce a visible signal to detect Ab binding by continually converting substrate(TMB) to it's oxidized form, which amplifies the signal frm the binding Ab mol'c. This produces a signal such as color or luminescence. [Question]
In the ELISA test, what is the role of the peroxidase?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It is is used as as the peroxidase conjugate on secondary Ab, it catalyzes the oxidation reaction of peroxide and luminol. [Question]
Why is HRP used in ELISA?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Shifts absorbance max giving a blue color. [Question]
What happens when TMB is oxidized?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] To stop the oxidation rxn of TMB. [Question]
Why did we add sulfuric acid in ELISA?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] nonspecific binding.is a negative control. some of the IP1peroxidase conjugate was added, but no primary antibody.the only signal would be from IP1-peroxidase conjugate that stuck "non-specifically" to the well, if for instance say it wasn't blocked well. [Question]
What does NBS stand for and what is it?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It is a positive control. The control ensures that the peroxidase conjugate still has catalytic activity. [Question]
What was the total activity control for?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] The part of an antigen that is recognized by the Ab. Where the Ab binds. An antigen can have more than one epitope. [Question]
What is an epitope?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] size, shape and charge [Question]
What are the 3 factors that determine how fast a molecule will migrate in the gel during electrophoresis?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] SDS, mercaptoethanol, and heat [Question]
What 3 things ensure that all proteins loaded onto the gel are completely denatured in SDS-PAGE?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] Electrolysis (splitting of water with elctricity). Ions in solution(buffer salts) create the current. 4e- + 4H2O->2H2 +4OH (at cathode). 2H2O->O2+4H+4e- [Question]
What produces the voltage across the electric field in electro phoresis? Give the rxn.
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] It is one of the rcts(substrates for peroxidase) [Question]
Why did we had H2O2 to the substrate solution during ECL visualization?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
36218ae2_Flashcards_about_MAPK__Question
[Answer] antigen must be recognized as not self and the antigen must be large enough. [Question]
What two conditions must be met to illicit an immune response?
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Flashcards about MAPK
Question
http://www.studystack.com/flashcard-412769
29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] corporation [Answer]
owned by stockholders
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Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] charter [Answer]
certificate of incorporation
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] stockholders [Answer]
shareholders
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Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] shares [Answer]
divisions of ownership
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Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] dividends [Answer]
division of profit
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] board of directors [Answer]
directors or board
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] officers [Answer]
run the company
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] cooperative [Answer]
owned by the customers
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] proxy [Answer]
able to act in another's absence
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
fdb143e3_rds_about_Business_Ownership_2__Answer
[Question] close corporation [Answer]
closely held corporation
[]
Flashcards about Business Ownership 2
Answer
http://www.studystack.com/flashcard-106079
29/1438042987628.47_20150728002307-00163-ip-10-236-191-2_713716849_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Large body of air with uniform temperature and moisture content [Question]
Air Mass
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Instrument used to measure wind speed [Question]
Anemometer
[]
Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Storm that spirals outward from a high-pressure center [Question]
Anticyclone
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Instrument used to measure temperature, consisting of a bar made of two strips of different metals that curves when heated and straightens when cooled [Question]
Bimetal Thermometer
[]
Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Boundary formed where a cold air mass overtakes and lifts a warm air mass [Question]
Cold Front
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Describing a cold, dry air mass that forms over land in polar regions [Question]
Continental Polar
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Describing a warm, dry air mass that forms over land in tropical regions [Question]
Continental Tropical
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Instrument used to measure temperature based on the increased flow of electricity through certain materials when the materials are heated [Question]
Electrical Thermometer
[]
Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Boundary between air masses of different densities [Question]
Front
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Severe storm that develops over tropical oceans, with strong winds that spiral in toward the intensely low-pressure storm center [Question]
Hurricane
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Line drawn on a weather map connecting points of equal atmospheric pressure [Question]
Isobar
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Describing a cold, moist air mass that forms over the ocean in polar areas [Question]
Maritime Polar
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Describing a warm, moist air mass that forms over the ocean in tropical areas [Question]
Maritime Tropical
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Boundary formed where a fast moving cold air mass overtakes and lifts a warm air mass, completely cutting it off from the ground [Question]
Occluded Front
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Boundary at which cold polar air meets the warmer air of the middle latitudes [Question]
Polar Front
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Device that can detect objects and weather conditions in the upper atmosphere by sending and reciving radio waves [Question]
Radar
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Long line of heavy thunderstorms that may occur just ahead of a fast-moving cold front [Question]
Squall line
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Cluster of weather symbols plotted on a map indicating the weather conditions at a particular reporting station [Question]
Station Model
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Boundary formed where two air masses meet and neither is displaced [Question]
Stationary Front
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Instrument that measures temperature changes by recording the movement of the bar of a bimetal thermometer [Question]
Thermograph
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Storm accompanied by thunder, lightning, and strong winds [Question]
Thunderstorm
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Whirling, funnel-shaped cyclone [Question]
Tornado
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Hurricane that forms over the Pacific Ocean [Question]
Typhoon
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Boundary formed where a warm air mass overtakes and rises over a cold air mass [Question]
Warm Front
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Tornado that occurs over the ocean [Question]
Waterspout
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Large storm that develops along cold or stationary fronts, with winds that spiral in toward a central region of low air pressure [Question]
Wave Cyclone
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
589df6e4_cards_about_Science_Chapter_25__Question
[Answer] Instrument used to determine the direction of the wind [Question]
Wind Vane
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Flashcards about Science Chapter 25
Question
http://www.studystack.com/flashcard-807074
29/1438042987628.47_20150728002307-00241-ip-10-236-191-2_715473282_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] without feeling [sentences] Ponyboy walked ____ home after the death of Johnny because he did not know how to feel as he seemed to feel nothing. [terms]
numbly
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] without direction, wandering [sentences] Dally ___ wandered the streets looking after leaving the hospital because he did not know where to go. [terms]
aimlessly
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] arguing, disputing, or discussing [sentences] The brothers were ____ whether or not to let Ponyboy be in the rumble. [terms]
debating
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] closing tight (as with your hand when you make a fist) [sentences] _____ his fist, Dally slammed it into the door as he left the hospital. [terms]
clenching
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] twisting of the face (possibly because you are in agony) [sentences] Johnny's _____ face showed that he was in a lot of pain. [terms]
grimacing
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] small replica (small copy for ex. a small model of a building that will be built) [sentences] The boy built a small ____ model of a Model T car. [terms]
miniature
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] prison or school for lawbreakers [sentences] Dally spent several years in a ____ school because he broke the law so much. [terms]
reformatory
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] feeling like you are better than someone else [sentences] The Socs' _____ made the Greasers angry because they felt belittled by the Socs. [terms]
superiority
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] gymnastic feats (cartwheels, rolls, headstands) [sentences] The Greasers left the house doing ____ over the porch rails and across the lawn. [terms]
acrobatics
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] surroundings (nature, where you live, etc.)surroundings [sentences] The countryside was a peaceful ____ for the Johnny and Ponyboy but Ponyboy still missed home. [terms]
environment
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] without opportunity (possible poor/without money for needs) [sentences] The Greasers seemed to be the ____ group that had little opportunities in comparision to the Socs. [terms]
underpriviledged
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] hold back, keep from happening (as in a sneeze that you try to hold back) [sentences] When Sodapop was teasing the cops, everyone else _____ the desire to burst into laughter. [terms]
stifled
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Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] unsure or afraid [sentences] Johnny was ____ of any Socs because he had been beat up before. [terms]
leery
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] disdain, dislike, disrespect [sentences] Dally had complete ____ for the law and did as he pleased. [terms]
contempt
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] filled with displeasure, not happy about [sentences] The girls were ____ by Dally's behavior at the movies, so Johnny tried to get him to stop bothering them. [terms]
disgusted
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] still, stationary, motionless [sentences] As the sun rose, there was a ___ moment where every thing was still and golden. [terms]
static
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
8736c725_Flashcards_about_Blair_s_Test__terms
[definitions] pain [sentences] The burns caused Johnny a lot of ____. [terms]
agony
[]
Flashcards about Blair's Test
terms
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
24281610_Flashcards_about_Blair_s_Test__sentences
[terms] numbly [definitions] without feeling [sentences]
Ponyboy walked ____ home after the death of Johnny because he did not know how to feel as he seemed to feel nothing.
[]
Flashcards about Blair's Test
sentences
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json
24281610_Flashcards_about_Blair_s_Test__sentences
[terms] aimlessly [definitions] without direction, wandering [sentences]
Dally ___ wandered the streets looking after leaving the hospital because he did not know where to go.
[]
Flashcards about Blair's Test
sentences
http://www.studystack.com/flashcard-324838
25/1438042987155.85_20150728002307-00207-ip-10-236-191-2_732073957_3.json