stanford-murine: restructure the data card to the common layout

#55
Files changed (1) hide show
  1. stanford-murine/README.md +64 -138
stanford-murine/README.md CHANGED
@@ -1,4 +1,5 @@
1
  ---
 
2
  license: cc-by-4.0
3
  pretty_name: Stanford Murine Liver and Sound-Speed Phantom Ultrasound
4
  task_categories:
@@ -24,28 +25,33 @@ size_categories:
24
 
25
  ## Dataset Description
26
 
27
- This dataset contains pre-beamformed pulse-echo ultrasound channel data from
28
- murine livers and sound-speed phantoms. The data were acquired on a Verasonics
29
- Vantage 256 using multifocal, Hadamard-encoded, and full synthetic aperture
30
- (FSA) transmit sequences. The dataset supports research on beamforming,
31
- sound-speed estimation, and aberration correction; it is not intended for
32
- clinical diagnosis.
33
 
34
  The source data are available from Figshare:
35
 
36
  - [Murine liver acquisitions](https://doi.org/10.25452/figshare.plus.28291985)
37
  - [Sound-speed phantom and meat-layer acquisitions](https://doi.org/10.25452/figshare.plus.28291988)
38
 
39
- The converted dataset is hosted at
40
- [nvidia/OpenH-RF/stanford-murine](https://huggingface.co/datasets/nvidia/OpenH-RF/tree/main/stanford-murine).
41
 
42
- ## Dataset Contributors
43
 
44
- The source datasets were created at Stanford University by Arsenii V.
45
- Telichko, Rehman Ali, Andrew Andrzejek, Thurston Brevett, Benjamin N. Frey,
46
- Brian Boitnott, Jihye Baek, Louise Zhuang, Hoda Hashemi, Jun Hong Park, Caelia
47
- Thomas, and Jeremy Dahl. The contributing organization is the Dahl Lab,
48
- Stanford University. Contact: `jjdahl@stanford.edu`.
 
 
 
 
 
 
 
 
 
 
 
49
 
50
  ## Dataset Creation Date
51
 
@@ -53,7 +59,7 @@ Stanford University. Contact: `jjdahl@stanford.edu`.
53
 
54
  ## License / Terms of Use
55
 
56
- This dataset is provided in OpenH-RF under the Creative Commons Attribution 4.0 International License (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/). The original Figshare releases were published under Apache 2.0.
57
 
58
  ## Intended Usage
59
 
@@ -64,33 +70,24 @@ The data are intended for development and validation of:
64
  - aberration correction through tissue or meat layers; and
65
  - comparisons among multifocal, Hadamard-encoded, and FSA acquisition schemes.
66
 
67
- The provided pipeline is a reference delay-and-sum B-mode reconstruction, not
68
- a prescribed preprocessing pipeline for model training.
69
 
70
  ## Dataset Characterization
71
 
72
  ### Data Collection Method
73
 
74
- The collection combines in-vivo and post-mortem animal acquisitions with
75
- table-top phantom acquisitions:
76
 
77
- - The murine study contains liver scans from 20 Zucker rats: 4 lean controls
78
- and 16 obese animals used as a model of hepatic steatosis.
79
- - The phantom study contains an ATS 549 phantom and six agarose/graphite
80
- phantoms with varying n-propanol concentration. Many acquisitions include a
81
- 10–15 mm porcine or galline meat layer as a controlled aberrator.
82
 
83
  ### Labeling Method
84
 
85
- No image, pixel-wise segmentation, or manual annotation labels are supplied.
86
- Rat-level sound-speed, fat-percentage, and histopathology measurements are
87
- derived from the source quantification workbooks when available. Phantom
88
- sound speeds were measured independently with a through-transmission setup.
89
 
90
  ### Acquisition System
91
 
92
- All acquisitions use a Verasonics Vantage 256 research scanner. The converted
93
- demo covers:
94
 
95
  | Probe | Geometry | Elements | Center frequency | Sampling frequency |
96
  |---|---|---:|---:|---:|
@@ -98,18 +95,17 @@ demo covers:
98
  | L12-3v | Linear | 192 | 6.0 or 7.813 MHz | 25.0 or 31.25 MHz |
99
  | L12-5 | Linear | 256 | 7.5 MHz | 31.25 MHz |
100
 
101
- Each output file bundles three tracks named `multifocal`, `hadamard`, and
102
- `synthetic_aperture`. Transmit delays, transmit apodization, focal distances,
103
- origins, receive initial times, probe geometry, acquisition sound speed, and
104
- sampling frequencies are read from the corresponding MAT file.
105
- For the rat acquisitions, the Verasonics sound-speed setting is 1540 m/s; this
106
- is retained in `scan/sound_speed` for reconstruction. It is distinct from the
107
- post-acquisition liver sound-speed measurements described below.
108
 
109
  ## Dataset Format
110
 
111
- Converted data use the zea HDF5 representation. Channel samples have dimension
112
- order:
 
113
 
114
  ```text
115
  (n_frames, n_tx, n_ax, n_el, n_ch)
@@ -117,34 +113,23 @@ order:
117
 
118
  The converter:
119
 
120
- 1. reads every acquired Verasonics receive-buffer frame, limited to the sample
121
- interval referenced by each sequence's Receive metadata;
122
- 2. coherently averages duplicate physical elements from overlapping receive
123
- apertures while retaining full amplitude for elements recorded once;
124
  3. decodes the positive/negative Hadamard acquisitions;
125
- 4. trims axial samples that are zero across every frame, transmit, element, and
126
- channel; and
127
  5. stores the resulting RF channel data as `float32`.
128
 
129
- The `float32` dtype is intentional. Coherent aperture averaging can create
130
- half-integer samples, and Hadamard decoding can exceed the `int16` range.
131
- Saving decoded RF as `int16` would round or overflow valid samples.
132
 
133
- Reconstructed B-mode images and segmentation masks are not stored in the
134
- converted files. They are generated separately by the validation pipeline.
135
 
136
  ## Dataset Quantification
137
 
138
  **Current OpenH-RF release:** 245 HDF5 files; 328.99 GB (328,985,804,800 bytes) stored; root `zea_version` **0.1.4**. Sizes include all HDF5 contents and use decimal units (MB = 10^6 bytes, GB = 10^9 bytes, TB = 10^12 bytes), not decoded-array memory or original-source download sizes.
139
 
140
- The original four-file demo (not the complete HF release summarized above) contains four HDF5 acquisition bundles: one Rat9 bundle
141
- and one phantom bundle for each of C5-2v, L12-3v, and L12-5. Each bundle has
142
- three sequence tracks, and each track retains every frame in its source MAT
143
- file. That demo has 12 tracks and 64 frames and occupies approximately
144
- 5.5 GiB; HDF5 writer and compression versions may change the exact byte count.
145
 
146
- The two complete source Figshare records contain approximately 491.9 GB in
147
- total. No train, validation, or test split is defined. The current converted-release size and file count are reported above; the scripts do not assign learning splits.
148
 
149
  Representative `raw_data` shapes in the demo are:
150
 
@@ -155,13 +140,7 @@ Representative `raw_data` shapes in the demo are:
155
  | Phantom, L12-3v | `(2, 576, 1613, 192, 1)` | `(2, 192, 1613, 192, 1)` | `(10, 192, 1613, 192, 1)` |
156
  | Phantom, L12-5 | `(2, 512, 2018, 256, 1)` | `(2, 256, 2018, 256, 1)` | `(2, 256, 2018, 256, 1)` |
157
 
158
- The Rat9 multifocal sequence uses the three focal depths programmed in the
159
- source MAT file: 17.74, 29.57, and 41.39 mm. Its programmed imaging range
160
- extends to 50.46 mm, so all three foci are intentional. The stored RF tensor
161
- ends near 21.24 mm because samples beyond that depth are zero across every
162
- transmit and receive channel and the converter removes this all-zero tail.
163
- The focal metadata are retained even when a focus lies beyond the remaining
164
- nonzero RF support.
165
 
166
  Core per-track features are:
167
 
@@ -181,12 +160,7 @@ Core per-track features are:
181
 
182
  ## Subject Metadata
183
 
184
- The source murine cohort contains 4 lean Zucker rats (2 male and 2 female) and
185
- 16 obese Zucker rats (8 male and 8 female), beginning at 13 weeks of age. The
186
- obese animals were fed a high-fat diet for up to eight weeks. Converted rat
187
- files store the rat identifier and, when present in the source workbooks, sex,
188
- fat percentage, measured liver sound speed, weight, diet duration, and
189
- histopathology-derived values.
190
 
191
  Values with standard DataSpec fields are stored under `metadata/subject`:
192
 
@@ -196,30 +170,13 @@ Values with standard DataSpec fields are stored under `metadata/subject`:
196
  | `weight` | `float32` | kg | Animal weight, converted from workbook grams |
197
  | `sex` | string | unitless | Sex reported by the source workbook |
198
 
199
- Other workbook measurements are scalar `float32` datasets under
200
- `custom/rat_quantification/`, each with `unit` and `description` attributes.
201
- These include the ground-truth sound-speed mean and standard deviation,
202
- sequence-specific local and global sound-speed estimates, liver-lobe
203
- sound-speed measurements when available, diet duration, fat-percentage
204
- uncertainty, water temperature, and quantitative histopathology scores. The
205
- workbook's non-fat percentage is omitted because it is exactly
206
- `100 - metadata/subject/fat_percentage` for every rat. These measurements are
207
- scalars rather than homogeneous `sos_map` images because the source provides
208
- no spatial coordinates for these rat-level measurements.
209
-
210
- The phantom data contain no human subjects. Phantom identifiers describe the
211
- probe, material configuration, meat layer, and acquisition session. The source
212
- records report independently measured phantom and meat-layer sound speeds and
213
- temperature-dependent uncertainties.
214
 
215
  ## Data Validation
216
 
217
- The reconstruction path uses the first stored frame and a zea `Pipeline`: RF
218
- demodulation and baseband FIR filtering, delay-and-sum beamforming, envelope
219
- detection, maximum normalization, and log compression. The pipeline YAML files
220
- use three pixels per acoustic wavelength and disable pressure-field weighting.
221
- Beamforming is split into bounded patches so the deepest C5-2v grid fits on a
222
- 24 GB GPU.
223
 
224
  From the repository root, generate the demo files and reference images with:
225
 
@@ -237,8 +194,7 @@ python examples/stanford/stitch.py --dataset rat --demo --rat-id 9
237
  python examples/stanford/stitch.py --dataset phantom --demo
238
  ```
239
 
240
- The reconstructed PNGs and demodulated spectra are written under
241
- `examples/stanford/outputs`. The stitched review documents are:
242
 
243
  - `examples/stanford/outputs/RatExperiments/all_bmode_reconstructions.pdf`
244
  - `examples/stanford/outputs/SoSExperiments/all_sos_bmode_reconstructions.pdf`
@@ -247,39 +203,22 @@ The reconstruction scripts are validated with zea 0.1.4.
247
 
248
  ## Known Issues
249
 
250
- - The demo MAT files store `Receive/TGC` profile index 1 but do not store the
251
- selected profile's `TGC/Waveform`. A profile index is not a gain curve, so
252
- the converter omits the optional `scan/tgc_gain_curve` field rather than
253
- inventing a unity curve. The RF samples already reflect acquisition-time
254
- TGC, but that gain cannot be calibrated or undone from these files alone.
255
- - Respiratory motion affected early in-vivo rat acquisitions. Later
256
- acquisitions were made shortly after euthanasia, as described by the source
257
- record and associated publication.
258
- - The source record notes settling and slight inhomogeneity in phantom 4 and
259
- phantom 5.
260
- - Strong meat/phantom interfaces and curved-probe reverberation can dominate
261
- individual B-mode images. Reconstruction limits preserve the acquired depth
262
- rather than cropping those regions automatically.
263
  - The data contain no image or segmentation ground truth.
264
 
265
  ## Ethical Considerations
266
 
267
- No human data are included. The murine study was approved by Stanford
268
- University's Institutional Administrative Panel on Laboratory Animal Care.
269
- Animals were scanned under 2% isoflurane anesthesia on a heated platform with
270
- continuous temperature monitoring; the associated publication describes the
271
- euthanasia and tissue-measurement procedures. The public sources do not state
272
- an approval number or explicit ARRIVE 2.0 compliance.
273
 
274
- The phantom acquisitions require no human- or animal-subject approval. Both
275
- Figshare records confirm that no human personally identifiable information is
276
- present.
277
 
278
  ## Scripts and Paths
279
 
280
  - `download.py`: download the raw Figshare demo subset or full records.
281
- - `download_figshare_full.sh`: resumably mirror both full Figshare records to
282
- `/ultra20/figshare` with progress and checksum verification.
283
  - `convert.py`: convert source MAT files to zea HDF5.
284
  - `reconstruct.py`: reconstruct HDF5 tracks to B-mode PNGs and spectra.
285
  - `stitch.py`: combine PNGs into review PDFs.
@@ -306,39 +245,26 @@ python examples/stanford/stitch.py --dataset rat --full
306
  python examples/stanford/stitch.py --dataset phantom --full
307
  ```
308
 
309
- To resume an interrupted conversion without rewriting completed bundles, add
310
- `--skip-existing`. A bundle is skipped only when both its atomic HDF5 output
311
- and text summary are present.
312
 
313
- To keep a separate, resumable mirror of both complete Figshare records under
314
- `/ultra20/figshare`, with per-file and overall progress bars plus size and MD5
315
- verification, run:
316
 
317
  ```bash
318
  examples/stanford/download_figshare_full.sh
319
  ```
320
 
321
- This writes `RatExperiments_download` and `SoSExperiments_download`. Use
322
- `examples/stanford/download_figshare_full.sh --dry-run` to validate and
323
- summarize the public records without downloading them.
324
 
325
- Use `--min-rat N` or `--rat-id N` with `convert.py` to limit rat conversion.
326
- Raw MAT files remain local; `upload.py` selects converted HDF5 files and
327
- documentation explicitly.
328
 
329
  ## Citation
330
 
331
  Please cite the applicable source dataset and its associated publication:
332
 
333
- - Telichko, A. V. et al. (2025). *Pulse-Echo Ultrasound Murine In Vivo
334
- Acquisitions*. Figshare+. <https://doi.org/10.25452/figshare.plus.28291985.v1>
335
- - Ali, R. et al. (2025). *Pulse-Echo Ultrasound Sound Speed Phantom and Meat
336
- Aberrating Layer Acquisitions*. Figshare+.
337
  <https://doi.org/10.25452/figshare.plus.28291988.v1>
338
- - Telichko, A. V. et al. “Noninvasive Estimation of Local Speed of Sound by
339
- Pulse-Echo Ultrasound in a Rat Model of Nonalcoholic Fatty Liver.” *Physics
340
- in Medicine & Biology* 67(1), 015007 (2022).
341
  <https://doi.org/10.1088/1361-6560/ac4562>
342
- - Ali, R. et al. “Local Sound Speed Estimation for Pulse-Echo Ultrasound in
343
- Layered Media.” *IEEE TUFFC* 69(2), 500–511 (2022).
344
  <https://doi.org/10.1109/TUFFC.2021.3124479>
 
1
  ---
2
+ name: stanford-murine
3
  license: cc-by-4.0
4
  pretty_name: Stanford Murine Liver and Sound-Speed Phantom Ultrasound
5
  task_categories:
 
25
 
26
  ## Dataset Description
27
 
28
+ This dataset contains pre-beamformed pulse-echo ultrasound channel data from murine livers and sound-speed phantoms. The data were acquired on a Verasonics Vantage 256 using multifocal, Hadamard-encoded, and full synthetic aperture (FSA) transmit sequences. The dataset supports research on beamforming, sound-speed estimation, and aberration correction; it is not intended for clinical diagnosis.
 
 
 
 
 
29
 
30
  The source data are available from Figshare:
31
 
32
  - [Murine liver acquisitions](https://doi.org/10.25452/figshare.plus.28291985)
33
  - [Sound-speed phantom and meat-layer acquisitions](https://doi.org/10.25452/figshare.plus.28291988)
34
 
35
+ The original Figshare releases were published under Apache 2.0; this converted release is CC BY 4.0.
 
36
 
37
+ The converted dataset is hosted at [nvidia/OpenH-RF/stanford-murine](https://huggingface.co/datasets/nvidia/OpenH-RF/tree/main/stanford-murine).
38
 
39
+ ## Dataset Contributor(s)
40
+
41
+ The source datasets were created at Stanford University (Dahl Lab):
42
+
43
+ - Arsenii V. Telichko
44
+ - Rehman Ali
45
+ - Andrew Andrzejek
46
+ - Thurston Brevett
47
+ - Benjamin N. Frey
48
+ - Brian Boitnott
49
+ - Jihye Baek
50
+ - Louise Zhuang
51
+ - Hoda Hashemi
52
+ - Jun Hong Park
53
+ - Caelia Thomas
54
+ - Jeremy Dahl <jjdahl@stanford.edu> (contact)
55
 
56
  ## Dataset Creation Date
57
 
 
59
 
60
  ## License / Terms of Use
61
 
62
+ [Creative Commons Attribution 4.0 International (CC BY 4.0)](https://creativecommons.org/licenses/by/4.0/legalcode.en). Retain attribution and identify modifications when reusing the data.
63
 
64
  ## Intended Usage
65
 
 
70
  - aberration correction through tissue or meat layers; and
71
  - comparisons among multifocal, Hadamard-encoded, and FSA acquisition schemes.
72
 
73
+ The provided pipeline is a reference delay-and-sum B-mode reconstruction, not a prescribed preprocessing pipeline for model training.
 
74
 
75
  ## Dataset Characterization
76
 
77
  ### Data Collection Method
78
 
79
+ The collection combines in-vivo and post-mortem animal acquisitions with table-top phantom acquisitions:
 
80
 
81
+ - The murine study contains liver scans from 20 Zucker rats: 4 lean controls and 16 obese animals used as a model of hepatic steatosis.
82
+ - The phantom study contains an ATS 549 phantom and six agarose/graphite phantoms with varying n-propanol concentration. Many acquisitions include a 10–15 mm porcine or galline meat layer as a controlled aberrator.
 
 
 
83
 
84
  ### Labeling Method
85
 
86
+ No image, pixel-wise segmentation, or manual annotation labels are supplied. Rat-level sound-speed, fat-percentage, and histopathology measurements are derived from the source quantification workbooks when available. Phantom sound speeds were measured independently with a through-transmission setup.
 
 
 
87
 
88
  ### Acquisition System
89
 
90
+ All acquisitions use a Verasonics Vantage 256 research scanner. The converted demo covers:
 
91
 
92
  | Probe | Geometry | Elements | Center frequency | Sampling frequency |
93
  |---|---|---:|---:|---:|
 
95
  | L12-3v | Linear | 192 | 6.0 or 7.813 MHz | 25.0 or 31.25 MHz |
96
  | L12-5 | Linear | 256 | 7.5 MHz | 31.25 MHz |
97
 
98
+ Each output file bundles three tracks named `multifocal`, `hadamard`, and `synthetic_aperture`. Transmit delays, transmit apodization, focal distances, origins, receive initial times, probe geometry, acquisition sound speed, and sampling frequencies are read from the corresponding MAT file. For the rat acquisitions, the Verasonics sound-speed setting is 1540 m/s; this is retained in `scan/sound_speed` for reconstruction. It is distinct from the post-acquisition liver sound-speed measurements described below.
99
+
100
+ ## Processing the Dataset
101
+
102
+ The acquisitions can be processed with the `reconstruct.py` [script](https://github.com/open-h/OpenH-RF/blob/main/datasets/stanford-murine/reconstruct.py) as provided in the [OpenH-RF GitHub repository](https://github.com/open-h/OpenH-RF), together with the `pipeline_multifocal.yaml`, `pipeline_hadamard.yaml` and `pipeline_synthetic_aperture.yaml` definitions in this folder and the [zea library](https://github.com/tue-bmd/zea). The script streams the data from the Hugging Face Hub; each file bundles the three tracks, and the script reconstructs every track with its own pipeline and writes one image per track to `assets/`.
 
 
103
 
104
  ## Dataset Format
105
 
106
+ [zea v0.1.4](https://github.com/tue-bmd/zea)
107
+
108
+ Converted data use the zea HDF5 representation. Channel samples have dimension order:
109
 
110
  ```text
111
  (n_frames, n_tx, n_ax, n_el, n_ch)
 
113
 
114
  The converter:
115
 
116
+ 1. reads every acquired Verasonics receive-buffer frame, limited to the sample interval referenced by each sequence's Receive metadata;
117
+ 2. coherently averages duplicate physical elements from overlapping receive apertures while retaining full amplitude for elements recorded once;
 
 
118
  3. decodes the positive/negative Hadamard acquisitions;
119
+ 4. trims axial samples that are zero across every frame, transmit, element, and channel; and
 
120
  5. stores the resulting RF channel data as `float32`.
121
 
122
+ The `float32` dtype is intentional. Coherent aperture averaging can create half-integer samples, and Hadamard decoding can exceed the `int16` range. Saving decoded RF as `int16` would round or overflow valid samples.
 
 
123
 
124
+ Reconstructed B-mode images and segmentation masks are not stored in the converted files. They are generated separately by the validation pipeline.
 
125
 
126
  ## Dataset Quantification
127
 
128
  **Current OpenH-RF release:** 245 HDF5 files; 328.99 GB (328,985,804,800 bytes) stored; root `zea_version` **0.1.4**. Sizes include all HDF5 contents and use decimal units (MB = 10^6 bytes, GB = 10^9 bytes, TB = 10^12 bytes), not decoded-array memory or original-source download sizes.
129
 
130
+ The original four-file demo (not the complete HF release summarized above) contains four HDF5 acquisition bundles: one Rat9 bundle and one phantom bundle for each of C5-2v, L12-3v, and L12-5. Each bundle has three sequence tracks, and each track retains every frame in its source MAT file. That demo has 12 tracks and 64 frames and occupies approximately 5.5 GiB; HDF5 writer and compression versions may change the exact byte count.
 
 
 
 
131
 
132
+ The two complete source Figshare records contain approximately 491.9 GB in total. No train, validation, or test split is defined. The current converted-release size and file count are reported above; the scripts do not assign learning splits.
 
133
 
134
  Representative `raw_data` shapes in the demo are:
135
 
 
140
  | Phantom, L12-3v | `(2, 576, 1613, 192, 1)` | `(2, 192, 1613, 192, 1)` | `(10, 192, 1613, 192, 1)` |
141
  | Phantom, L12-5 | `(2, 512, 2018, 256, 1)` | `(2, 256, 2018, 256, 1)` | `(2, 256, 2018, 256, 1)` |
142
 
143
+ The Rat9 multifocal sequence uses the three focal depths programmed in the source MAT file: 17.74, 29.57, and 41.39 mm. Its programmed imaging range extends to 50.46 mm, so all three foci are intentional. The stored RF tensor ends near 21.24 mm because samples beyond that depth are zero across every transmit and receive channel and the converter removes this all-zero tail. The focal metadata are retained even when a focus lies beyond the remaining nonzero RF support.
 
 
 
 
 
 
144
 
145
  Core per-track features are:
146
 
 
160
 
161
  ## Subject Metadata
162
 
163
+ The source murine cohort contains 4 lean Zucker rats (2 male and 2 female) and 16 obese Zucker rats (8 male and 8 female), beginning at 13 weeks of age. The obese animals were fed a high-fat diet for up to eight weeks. Converted rat files store the rat identifier and, when present in the source workbooks, sex, fat percentage, measured liver sound speed, weight, diet duration, and histopathology-derived values.
 
 
 
 
 
164
 
165
  Values with standard DataSpec fields are stored under `metadata/subject`:
166
 
 
170
  | `weight` | `float32` | kg | Animal weight, converted from workbook grams |
171
  | `sex` | string | unitless | Sex reported by the source workbook |
172
 
173
+ Other workbook measurements are scalar `float32` datasets under `custom/rat_quantification/`, each with `unit` and `description` attributes. These include the ground-truth sound-speed mean and standard deviation, sequence-specific local and global sound-speed estimates, liver-lobe sound-speed measurements when available, diet duration, fat-percentage uncertainty, water temperature, and quantitative histopathology scores. The workbook's non-fat percentage is omitted because it is exactly `100 - metadata/subject/fat_percentage` for every rat. These measurements are scalars rather than homogeneous `sos_map` images because the source provides no spatial coordinates for these rat-level measurements.
174
+
175
+ The phantom data contain no human subjects. Phantom identifiers describe the probe, material configuration, meat layer, and acquisition session. The source records report independently measured phantom and meat-layer sound speeds and temperature-dependent uncertainties.
 
 
 
 
 
 
 
 
 
 
 
 
176
 
177
  ## Data Validation
178
 
179
+ The reconstruction path uses the first stored frame and a zea `Pipeline`: RF demodulation and baseband FIR filtering, delay-and-sum beamforming, envelope detection, maximum normalization, and log compression. The pipeline YAML files use three pixels per acoustic wavelength and disable pressure-field weighting. Beamforming is split into bounded patches so the deepest C5-2v grid fits on a 24 GB GPU.
 
 
 
 
 
180
 
181
  From the repository root, generate the demo files and reference images with:
182
 
 
194
  python examples/stanford/stitch.py --dataset phantom --demo
195
  ```
196
 
197
+ The reconstructed PNGs and demodulated spectra are written under `examples/stanford/outputs`. The stitched review documents are:
 
198
 
199
  - `examples/stanford/outputs/RatExperiments/all_bmode_reconstructions.pdf`
200
  - `examples/stanford/outputs/SoSExperiments/all_sos_bmode_reconstructions.pdf`
 
203
 
204
  ## Known Issues
205
 
206
+ - The demo MAT files store `Receive/TGC` profile index 1 but do not store the selected profile's `TGC/Waveform`. A profile index is not a gain curve, so the converter omits the optional `scan/tgc_gain_curve` field rather than inventing a unity curve. The RF samples already reflect acquisition-time TGC, but that gain cannot be calibrated or undone from these files alone.
207
+ - Respiratory motion affected early in-vivo rat acquisitions. Later acquisitions were made shortly after euthanasia, as described by the source record and associated publication.
208
+ - The source record notes settling and slight inhomogeneity in phantom 4 and phantom 5.
209
+ - Strong meat/phantom interfaces and curved-probe reverberation can dominate individual B-mode images. Reconstruction limits preserve the acquired depth rather than cropping those regions automatically.
 
 
 
 
 
 
 
 
 
210
  - The data contain no image or segmentation ground truth.
211
 
212
  ## Ethical Considerations
213
 
214
+ No human data are included. The murine study was approved by Stanford University's Institutional Administrative Panel on Laboratory Animal Care. Animals were scanned under 2% isoflurane anesthesia on a heated platform with continuous temperature monitoring; the associated publication describes the euthanasia and tissue-measurement procedures. The public sources do not state an approval number or explicit ARRIVE 2.0 compliance.
 
 
 
 
 
215
 
216
+ The phantom acquisitions require no human- or animal-subject approval. Both Figshare records confirm that no human personally identifiable information is present.
 
 
217
 
218
  ## Scripts and Paths
219
 
220
  - `download.py`: download the raw Figshare demo subset or full records.
221
+ - `download_figshare_full.sh`: resumably mirror both full Figshare records to `/ultra20/figshare` with progress and checksum verification.
 
222
  - `convert.py`: convert source MAT files to zea HDF5.
223
  - `reconstruct.py`: reconstruct HDF5 tracks to B-mode PNGs and spectra.
224
  - `stitch.py`: combine PNGs into review PDFs.
 
245
  python examples/stanford/stitch.py --dataset phantom --full
246
  ```
247
 
248
+ To resume an interrupted conversion without rewriting completed bundles, add `--skip-existing`. A bundle is skipped only when both its atomic HDF5 output and text summary are present.
 
 
249
 
250
+ To keep a separate, resumable mirror of both complete Figshare records under `/ultra20/figshare`, with per-file and overall progress bars plus size and MD5 verification, run:
 
 
251
 
252
  ```bash
253
  examples/stanford/download_figshare_full.sh
254
  ```
255
 
256
+ This writes `RatExperiments_download` and `SoSExperiments_download`. Use `examples/stanford/download_figshare_full.sh --dry-run` to validate and summarize the public records without downloading them.
 
 
257
 
258
+ Use `--min-rat N` or `--rat-id N` with `convert.py` to limit rat conversion. Raw MAT files remain local; `upload.py` selects converted HDF5 files and documentation explicitly.
 
 
259
 
260
  ## Citation
261
 
262
  Please cite the applicable source dataset and its associated publication:
263
 
264
+ - Telichko, A. V. et al. (2025). *Pulse-Echo Ultrasound Murine In Vivo Acquisitions*. Figshare+. <https://doi.org/10.25452/figshare.plus.28291985.v1>
265
+ - Ali, R. et al. (2025). *Pulse-Echo Ultrasound Sound Speed Phantom and Meat Aberrating Layer Acquisitions*. Figshare+.
 
 
266
  <https://doi.org/10.25452/figshare.plus.28291988.v1>
267
+ - Telichko, A. V. et al. “Noninvasive Estimation of Local Speed of Sound by Pulse-Echo Ultrasound in a Rat Model of Nonalcoholic Fatty Liver.” *Physics in Medicine & Biology* 67(1), 015007 (2022).
 
 
268
  <https://doi.org/10.1088/1361-6560/ac4562>
269
+ - Ali, R. et al. “Local Sound Speed Estimation for Pulse-Echo Ultrasound in Layered Media.” *IEEE TUFFC* 69(2), 500–511 (2022).
 
270
  <https://doi.org/10.1109/TUFFC.2021.3124479>