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# Make a list of all task configurations needed to supervise sup_configs = sorted(dependencies.keys()) try: logging.info("Validating supervisor data...") # Make an api call to list task configurations in the workspace r = fapi.list_workspace_configs(args['project'], args['work...
def validate_monitor_tasks(dependencies, args)
Validate that all entries in the supervisor are valid task configurations and that all permissions requirements are satisfied.
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try: logging.info("Attempting to recover Supervisor data from " + recovery_file) with open(recovery_file) as rf: recovery_data = json.load(rf) monitor_data = recovery_data['monitor_data'] dependencies = recovery_data['dependencies'] args = recover...
def recover_and_supervise(recovery_file)
Retrieve monitor data from recovery_file and resume monitoring
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''' List accessible workspaces, in TSV form: <namespace><TAB>workspace''' r = fapi.list_workspaces() fapi._check_response_code(r, 200) spaces = [] project = args.project if project: project = re.compile('^' + project) for space in r.json(): ns = space['workspace']['namespa...
def space_list(args)
List accessible workspaces, in TSV form: <namespace><TAB>workspace
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# The return value is the INVERSE of UNIX exit status semantics, (where # 0 = good/true, 1 = bad/false), so to check existence in UNIX one would do # if ! fissfc space_exists blah ; then # ... # fi try: r = fapi.get_workspace(args.project, args.workspace) fapi._chec...
def space_exists(args)
Determine if the named space exists in the given project (namespace)
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r = fapi.lock_workspace(args.project, args.workspace) fapi._check_response_code(r, 204) if fcconfig.verbosity: eprint('Locked workspace {0}/{1}'.format(args.project, args.workspace)) return 0
def space_lock(args)
Lock a workspace
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r = fapi.unlock_workspace(args.project, args.workspace) fapi._check_response_code(r, 204) if fcconfig.verbosity: eprint('Unlocked workspace {0}/{1}'.format(args.project,args.workspace)) return 0
def space_unlock(args)
Unlock a workspace
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r = fapi.create_workspace(args.project, args.workspace, args.authdomain, dict()) fapi._check_response_code(r, 201) if fcconfig.verbosity: eprint(r.content) return 0
def space_new(args)
Create a new workspace.
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r = fapi.get_workspace(args.project, args.workspace) fapi._check_response_code(r, 200) return r.text
def space_info(args)
Get metadata for a workspace.
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message = "WARNING: this will delete workspace: \n\t{0}/{1}".format( args.project, args.workspace) if not args.yes and not _confirm_prompt(message): return 0 r = fapi.delete_workspace(args.project, args.workspace) fapi._check_response_code(r, [200, 202, 204, 404]) if fcconfig.v...
def space_delete(args)
Delete a workspace.
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# FIXME: add --deep copy option (shallow by default) # add aliasing capability, then make space_copy alias if not args.to_workspace: args.to_workspace = args.workspace if not args.to_project: args.to_project = args.project if (args.project == args.to_project and a...
def space_clone(args)
Replicate a workspace
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''' Retrieve access control list for a workspace''' r = fapi.get_workspace_acl(args.project, args.workspace) fapi._check_response_code(r, 200) result = dict() for user, info in sorted(r.json()['acl'].items()): result[user] = info['accessLevel'] return result
def space_acl(args)
Retrieve access control list for a workspace
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acl_updates = [{"email": user, "accessLevel": args.role} for user in args.users] r = fapi.update_workspace_acl(args.project, args.workspace, acl_updates) fapi._check_response_code(r, 200) errors = r.json()['usersNotFound'] if len(errors): eprint("Unable to assign rol...
def space_set_acl(args)
Assign an ACL role to list of users for a workspace
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r = fapi.list_workspaces() fapi._check_response_code(r, 200) # Parse the JSON for workspace + namespace; then filter by # search terms: each term is treated as a regular expression workspaces = r.json() extra_terms = [] if args.bucket: workspaces = [w for w in workspaces ...
def space_search(args)
Search for workspaces matching certain criteria
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project = args.project workspace = args.workspace chunk_size = args.chunk_size with open(args.tsvfile) as tsvf: headerline = tsvf.readline().strip() entity_data = [l.rstrip('\n') for l in tsvf] return _batch_load(project, workspace, headerline, entity_data, chunk_size)
def entity_import(args)
Upload an entity loadfile.
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'''Return a list of lines in TSV form that would suffice to reconstitute a container (set) entity, if passed to entity_import. The first line in the list is the header, and subsequent lines are the container members. ''' r = fapi.get_entity(args.project, args.workspace, args.entity_type, arg...
def set_export(args)
Return a list of lines in TSV form that would suffice to reconstitute a container (set) entity, if passed to entity_import. The first line in the list is the header, and subsequent lines are the container members.
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r = fapi.list_entity_types(args.project, args.workspace) fapi._check_response_code(r, 200) return r.json().keys()
def entity_types(args)
List entity types in a workspace
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r = fapi.get_entities_with_type(args.project, args.workspace) fapi._check_response_code(r, 200) return [ '{0}\t{1}'.format(e['entityType'], e['name']) for e in r.json() ]
def entity_list(args)
List entities in a workspace.
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''' List participants within a container''' # Case 1: retrieve participants within a named data entity if args.entity_type and args.entity: # Edge case: caller asked for participant within participant (itself) if args.entity_type == 'participant': return [ args.entity.strip() ] ...
def participant_list(args)
List participants within a container
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''' List pairs within a container. ''' # Case 1: retrieve pairs within a named data entity if args.entity_type and args.entity: # Edge case: caller asked for pair within a pair (itself) if args.entity_type == 'pair': return [ args.entity.strip() ] # Edge case: pairs for ...
def pair_list(args)
List pairs within a container.
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''' List samples within a container. ''' # Case 1: retrieve samples within a named data entity if args.entity_type and args.entity: # Edge case: caller asked for samples within a sample (itself) if args.entity_type == 'sample': return [ args.entity.strip() ] # Edge case:...
def sample_list(args)
List samples within a container.
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msg = "WARNING: this will delete {0} {1} in {2}/{3}".format( args.entity_type, args.entity, args.project, args.workspace) if not (args.yes or _confirm_prompt(msg)): return json_body=[{"entityType": args.entity_type, "entityName": args.entity}] r = fapi.delete_enti...
def entity_delete(args)
Delete entity in a workspace.
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r = fapi.update_repository_method(args.namespace, args.method, args.synopsis, args.wdl, args.doc, args.comment) fapi._check_response_code(r, 201) if fcconfig.verbosity: print("Method %s installed to project %s" % (args....
def meth_new(args)
Submit a new workflow (or update) to the methods repository.
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message = "WARNING: this will delete workflow \n\t{0}/{1}:{2}".format( args.namespace, args.method, args.snapshot_id) if not args.yes and not _confirm_prompt(message): return r = fapi.delete_repository_method(args.namespace, args.method, ...
def meth_delete(args)
Remove (redact) a method from the method repository
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''' Retrieve WDL for given version of a repository method''' r = fapi.get_repository_method(args.namespace, args.method, args.snapshot_id, True) fapi._check_response_code(r, 200) return r.text
def meth_wdl(args)
Retrieve WDL for given version of a repository method
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''' Retrieve access control list for given version of a repository method''' r = fapi.get_repository_method_acl(args.namespace, args.method, args.snapshot_id) fapi._check_response_code(r, 200) acls = sorted(r.json(), key=lambda k: k['user']) return...
def meth_acl(args)
Retrieve access control list for given version of a repository method
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acl_updates = [{"user": user, "role": args.role} \ for user in set(expand_fc_groups(args.users)) \ if user != fapi.whoami()] id = args.snapshot_id if not id: # get the latest snapshot_id for this method from the methods repo r = fapi.list_repositor...
def meth_set_acl(args)
Assign an ACL role to a list of users for a workflow.
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groups = None for user in users: fcgroup = None if '@' not in user: fcgroup = user elif user.lower().endswith('@firecloud.org'): if groups is None: r = fapi.get_groups() fapi._check_response_code(r, 200) groups ...
def expand_fc_groups(users)
If user is a firecloud group, return all members of the group. Caveat is that only group admins may do this.
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r = fapi.list_repository_methods(namespace=args.namespace, name=args.method, snapshotId=args.snapshot_id) fapi._check_response_code(r, 200) # Parse the JSON for the workspace + namespace methods = r.json() results = [] ...
def meth_list(args)
List workflows in the methods repository
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'''Invoke a task (method configuration), on given entity in given space''' # Try to use call caching (job avoidance)? Flexibly accept range of answers cache = getattr(args, "cache", True) cache = cache is True or (cache.lower() in ["y", "true", "yes", "t", "1"]) if not args.namespace: arg...
def config_start(args)
Invoke a task (method configuration), on given entity in given space
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'''Abort a task (method configuration) by submission ID in given space''' r = fapi.abort_submission(args.project, args.workspace, args.submission_id) fapi._check_response_code(r, 204) return ("Aborted {0} in {1}/{2}".format(args.submission_id, ...
def config_stop(args)
Abort a task (method configuration) by submission ID in given space
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verbose = fcconfig.verbosity if args.workspace: if verbose: print("Retrieving method configs from space {0}".format(args.workspace)) if not args.project: eprint("No project given, and no default project configured") return 1 r = fapi.list_workspac...
def config_list(args)
List configuration(s) in the methods repository or a workspace.
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''' Retrieve access control list for a method configuration''' r = fapi.get_repository_config_acl(args.namespace, args.config, args.snapshot_id) fapi._check_response_code(r, 200) acls = sorted(r.json(), key=lambda k: k['user']) return map(lambda ac...
def config_acl(args)
Retrieve access control list for a method configuration
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acl_updates = [{"user": user, "role": args.role} \ for user in set(expand_fc_groups(args.users)) \ if user != fapi.whoami()] id = args.snapshot_id if not id: # get the latest snapshot_id for this method from the methods repo r = fapi.list_repositor...
def config_set_acl(args)
Assign an ACL role to a list of users for a config.
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r = fapi.get_workspace_config(args.project, args.workspace, args.namespace, args.config) fapi._check_response_code(r, 200) # Setting ensure_ascii to False ensures unicode string returns return json.dumps(r.json(), indent=4, separators=(',', ': '), ...
def config_get(args)
Retrieve a method config from a workspace, send stdout
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r = fapi.get_workspace_config(args.project, args.workspace, args.namespace, args.config) fapi._check_response_code(r, 200) method = r.json()["methodRepoMethod"] args.namespace = method["methodNamespace"] args.method = method["methodName"] args.s...
def config_wdl(args)
Retrieve the WDL for a method config in a workspace, send stdout
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config_1 = config_get(args).splitlines() args.project = args.Project args.workspace = args.Workspace cfg_1_name = args.config if args.Config is not None: args.config = args.Config if args.Namespace is not None: args.namespace = args.Namespace config_2 = config_get(args)....
def config_diff(args)
Compare method configuration definitions across workspaces. Ignores methodConfigVersion if the verbose argument is not set
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'''Install a valid method configuration into a workspace, in one of several ways: from a JSON file containing a config definition (both file names and objects are supported); as a string representing the content of such a JSON file; or as a dict generated from such JSON content, e.g via ...
def config_put(args)
Install a valid method configuration into a workspace, in one of several ways: from a JSON file containing a config definition (both file names and objects are supported); as a string representing the content of such a JSON file; or as a dict generated from such JSON content, e.g via json.lo...
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'''Attempt to install a new method config into a workspace, by: generating a template from a versioned method in the methods repo, then launching a local editor (respecting the $EDITOR environment variable) to fill in the incomplete input/output fields. Returns True if the config was su...
def config_new(args)
Attempt to install a new method config into a workspace, by: generating a template from a versioned method in the methods repo, then launching a local editor (respecting the $EDITOR environment variable) to fill in the incomplete input/output fields. Returns True if the config was successfu...
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r = fapi.delete_workspace_config(args.project, args.workspace, args.namespace, args.config) fapi._check_response_code(r, [200,204]) return r.text if r.text else None
def config_delete(args)
Remove a method config from a workspace
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if not (args.tospace or args.toname or args.toproject or args.tonamespace): raise RuntimeError('A new config name OR workspace OR project OR ' + 'namespace must be given (or all)') copy = fapi.get_workspace_config(args.fromproject, args.fromspace, ...
def config_copy(args)
Copy a method config to new name/space/namespace/project (or all 4)
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'''Return a dict of attribute name/value pairs: if entity name & type are specified then attributes will be retrieved from that entity, otherwise workspace-level attributes will be returned. By default all attributes attached to the given object will be returned, but a subset can be selected by spe...
def attr_get(args)
Return a dict of attribute name/value pairs: if entity name & type are specified then attributes will be retrieved from that entity, otherwise workspace-level attributes will be returned. By default all attributes attached to the given object will be returned, but a subset can be selected by specifying...
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'''Retrieve names of all attributes attached to a given object, either an entity (if entity type+name is provided) or workspace (if not)''' args.attributes = None result = attr_get(args) names = result.get("__header__",[]) if names: names = names[1:] else: names = result.k...
def attr_list(args)
Retrieve names of all attributes attached to a given object, either an entity (if entity type+name is provided) or workspace (if not)
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''' Set key=value attributes: if entity name & type are specified then attributes will be set upon that entity, otherwise the attribute will be set at the workspace level''' if args.entity_type and args.entity: prompt = "Set {0}={1} for {2}:{3} in {4}/{5}?\n[Y\\n]: ".format( ...
def attr_set(args)
Set key=value attributes: if entity name & type are specified then attributes will be set upon that entity, otherwise the attribute will be set at the workspace level
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''' Delete key=value attributes: if entity name & type are specified then attributes will be deleted from that entity, otherwise the attribute will be removed from the workspace''' if args.entity_type and args.entities: # Since there is no attribute deletion endpoint, we must perform 2 steps ...
def attr_delete(args)
Delete key=value attributes: if entity name & type are specified then attributes will be deleted from that entity, otherwise the attribute will be removed from the workspace
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if not args.to_workspace: args.to_workspace = args.workspace if not args.to_project: args.to_project = args.project if (args.project == args.to_project and args.workspace == args.to_workspace): eprint("destination project and namespace must differ from" " ...
def attr_copy(args)
Copy workspace attributes between workspaces.
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r = fapi.health() fapi._check_response_code(r, 200) return r.content
def health(args)
Health FireCloud Server
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''' Loop over all sample sets in a workspace, performing a func ''' # Ensure that the requested action is a valid fiss_cmd fiss_func = __cmd_to_func(args.action) if not fiss_func: eprint("invalid FISS cmd '" + args.action + "'") return 1 # First get the sample set names r = fapi...
def sset_loop(args)
Loop over all sample sets in a workspace, performing a func
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''' Retrieve status of jobs submitted from a given workspace, as a list of TSV lines sorted by descending order of job submission date''' r = fapi.list_submissions(args.project, args.workspace) fapi._check_response_code(r, 200) statuses = sorted(r.json(), key=lambda k: k['submissionDate'], rever...
def monitor(args)
Retrieve status of jobs submitted from a given workspace, as a list of TSV lines sorted by descending order of job submission date
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''' Run legacy, Firehose-style workflow of workflows''' project = args.project workspace = args.workspace namespace = args.namespace workflow = args.workflow sample_sets = args.sample_sets recovery_file = args.json_checkpoint # If no sample sets are provided, run on all sample sets ...
def supervise(args)
Run legacy, Firehose-style workflow of workflows
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if not args.to_workspace: args.to_workspace = args.workspace if not args.to_project: args.to_project = args.project if (args.project == args.to_project and args.workspace == args.to_workspace): eprint("destination project and namespace must differ from" " ...
def entity_copy(args)
Copy entities from one workspace to another.
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'''Retrieve the list of billing projects accessible to the caller/user, and show the level of access granted for each (e.g. Owner, User, ...)''' projects = fapi.list_billing_projects() fapi._check_response_code(projects, 200) projects = sorted(projects.json(), key=lambda d: d['projectName']) ...
def proj_list(args)
Retrieve the list of billing projects accessible to the caller/user, and show the level of access granted for each (e.g. Owner, User, ...)
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''' Validate a workspace configuration: if an entity was specified (i.e. upon which the configuration should operate), then also validate that the entity has the necessary attributes''' r = fapi.validate_config(args.project, args.workspace, args.namespace, ...
def config_validate(args)
Validate a workspace configuration: if an entity was specified (i.e. upon which the configuration should operate), then also validate that the entity has the necessary attributes
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# 4 ways to have invalid config: invalid_inputs = sorted(config_d["invalidInputs"]) invalid_outputs = sorted(config_d["invalidOutputs"]) # Also insert values for invalid i/o invalid_inputs = [(i, config_d['methodConfiguration']['inputs'][i]) for i in invalid_inputs] invalid_outputs = [...
def _validate_helper(args, config_d, workspace_d, entity_d=None)
Return FISSFC validation information on config for a certain entity
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answer = input(message + prompt) return answer in affirmations
def _confirm_prompt(message, prompt="\nAre you sure? [y/yes (default: no)]: ", affirmations=("Y", "Yes", "yes", "y"))
Display a message, then confirmation prompt, and return true if the user responds with one of the affirmations.
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value = str(string) if len(value) == 0: msg = "No project provided and no default project configured" raise argparse.ArgumentTypeError(msg) return value
def _nonempty_project(string)
Argparse validator for ensuring a workspace is provided
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page = 1 all_entities = [] # Make initial request r = fapi.get_entities_query(namespace, workspace, etype, page=page, page_size=page_size, sort_direction=sort_direction, filter_terms=filter_terms) fapi._check_response_code(r, 200) resp...
def _entity_paginator(namespace, workspace, etype, page_size=500, filter_terms=None, sort_direction="asc")
Pages through the get_entities_query endpoint to get all entities in the workspace without crashing.
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fiss_module = sys.modules[__name__] # Returns None if string is not a recognized FISS command func = getattr(fiss_module, cmd, None) if func and not hasattr(func, 'fiss_cmd'): func = None return func
def __cmd_to_func(cmd)
Returns the function object in this module matching cmd.
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if not l: return False headers = l.split('\t') first_col = headers[0] tsplit = first_col.split(':') if len(tsplit) != 2: return False if tsplit[0] in ('entity', 'update'): return tsplit[1] in ('participant_id', 'participant_set_id', 's...
def _valid_headerline(l)
return true if the given string is a valid loadfile header
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if fcconfig.verbosity: print("Batching " + str(len(entity_data)) + " updates to Firecloud...") # Parse the entity type from the first cell, e.g. "entity:sample_id" # First check that the header is valid if not _valid_headerline(headerline): eprint("Invalid loadfile header:\n" + h...
def _batch_load(project, workspace, headerline, entity_data, chunk_size=500)
Submit a large number of entity updates in batches of chunk_size
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'''Use this entry point to call HL fiss funcs as though from the UNIX CLI. (see firecloud/tests/highlevel_tests.py:call_cli for usage examples)''' try: result = main(argv) except Exception as e: result = __pretty_print_fc_exception(e) # FIXME: we should invert True/False return va...
def main_as_cli(argv=None)
Use this entry point to call HL fiss funcs as though from the UNIX CLI. (see firecloud/tests/highlevel_tests.py:call_cli for usage examples)
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#First check that all entities are of the same type types = {e.etype for e in entities} if len(types) != 1: raise ValueError("Can't create payload with " + str(len(types)) + " types") all_attrs = set() for e in entities: ...
def create_payload(entities)
Create a tsv payload describing entities. A TSV payload consists of 1 header row describing entity type and attribute names. Each subsequent line is an entity_id followed by attribute values separated by the tab "\\t" character. This payload can be uploaded to the workspace via ...
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with open(f, 'w') as out: out.write(Entity.create_payload(entities))
def create_loadfile(entities, f)
Create payload and save to file.
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gcloud_default_path = ['google-cloud-sdk', 'bin'] if platform.system() != "Windows": gcloud_default_path = os.path.join(os.path.expanduser('~'), *gcloud_default_path) else: gcloud_default_path = os.path.join(os.envir...
def needs_gcloud(self)
Returns true if gcloud is unavailable and needed for authentication.
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for input_file in arguments.input_files: logging.info(input_file) # Generate a temporary file with common.atomic_write( input_file.name, file_factory=common.FileType('wt')) as tf: convert.transform_file(input_file, tf, arguments) if hasattr(input_...
def action(arguments)
Run mogrify. Most of the action is in convert, this just creates a temp file for the output.
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result = [[]] for c in sequence_str: result = [i + [a] for i in result for a in _AMBIGUOUS_MAP.get(c, c)] return [''.join(i) for i in result]
def all_unambiguous(sequence_str)
All unambiguous versions of sequence_str
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parser.add_argument( 'sequence_file', type=FileType('r'), help=) parser.add_argument( '--input-qual', type=FileType('r'), help=) parser.add_argument( 'output_file', type=FileType('w'), help=) output_group = parser.add_argument...
def build_parser(parser)
Generate a subparser
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it = iter(iterable) d = collections.deque(itertools.islice(it, n - 1)) d.appendleft(0) s = sum(d) for elem in it: s += elem - d.popleft() d.append(elem) yield s / float(n)
def moving_average(iterable, n)
From Python collections module documentation moving_average([40, 30, 50, 46, 39, 44]) --> 40.0 42.0 45.0 43.0
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tr = trie.trie() reader = csv.reader(fp) if header: # Skip header next(reader) # Skip blank rows records = (record for record in reader if record) for record in records: specimen, barcode = record[:2] if primer is not None: pr = primer ...
def parse_barcode_file(fp, primer=None, header=False)
Load label, barcode, primer records from a CSV file. Returns a map from barcode -> label Any additional columns are ignored
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if arguments.quality_window_mean_qual and not arguments.quality_window: raise ValueError("--quality-window-mean-qual specified without " "--quality-window") if trie is None or triefind is None: raise ValueError( 'Missing Bio.trie and/or Bio.triefind mod...
def action(arguments)
Given parsed arguments, filter input files.
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for record in iterable: self(name, record) yield record
def iterable_hook(self, name, iterable)
Fire an event named ``name`` with each item in iterable
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assert record.id == self.current_record['sequence_name'] self.current_record['sample'] = sample
def _found_barcode(self, record, sample, barcode=None)
Hook called when barcode is found
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for record in records: try: filtered = self.filter_record(record) assert (filtered) # Quick tracking whether the sequence was modified if filtered.seq == record.seq: self.passed_unchanged += 1 ...
def filter_records(self, records)
Apply the filter to records
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quality_scores = record.letter_annotations['phred_quality'] mean_score = mean(quality_scores) if mean_score >= self.min_mean_score: return record else: raise FailedFilter(mean_score)
def filter_record(self, record)
Filter a single record
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quality_scores = record.letter_annotations['phred_quality'] # Simple case - window covers whole sequence if len(record) <= self.window_size: mean_score = mean(quality_scores) if mean_score >= self.min_mean_score: return record else: ...
def filter_record(self, record)
Filter a single record
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nloc = record.seq.find('N') if nloc == -1: return record elif self.action == 'truncate': return record[:nloc] elif self.action == 'drop': raise FailedFilter() else: assert False
def filter_record(self, record)
Filter a record, truncating or dropping at an 'N'
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if len(record) >= self.min_length: return record else: raise FailedFilter(len(record))
def filter_record(self, record)
Filter record, dropping any that don't meet minimum length
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if len(record) >= self.max_length: return record[:self.max_length] else: return record
def filter_record(self, record)
Filter record, truncating any over some maximum length
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is_alignment = True avg_length = None min_length = sys.maxsize max_length = 0 sequence_count = 0 # Get an iterator and analyze the data. with common.FileType('rt')(source_file) as fp: if not file_type: file_type = fileformat.from_handle(fp) for record in Seq...
def summarize_sequence_file(source_file, file_type=None)
Summarizes a sequence file, returning a tuple containing the name, whether the file is an alignment, minimum sequence length, maximum sequence length, average length, number of sequences.
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# Ignore SIGPIPE, for head support common.exit_on_sigpipe() common.exit_on_sigint() handle = arguments.destination_file output_format = arguments.output_format if not output_format: try: output_format = 'align' if handle.isatty() else 'tab' except AttributeError...
def action(arguments)
Given one more more sequence files, determine if the file is an alignment, the maximum sequence length and the total number of sequences. Provides different output formats including tab (tab-delimited), csv and align (aligned as if part of a borderless table).
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with tempfile.SpooledTemporaryFile(buffer_size, mode='wb+') as tf: pickler = pickle.Pickler(tf) for record in records: pickler.dump(record) def record_iter(): tf.seek(0) unpickler = pickle.Unpickler(tf) while True: try: ...
def _record_buffer(records, buffer_size=DEFAULT_BUFFER_SIZE)
Buffer for transform functions which require multiple passes through data. Value returned by context manager is a function which returns an iterator through records.
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logging.info( "Applying _dashes_cleanup: converting any of '{}' to '-'.".format(prune_chars)) translation_table = {ord(c): '-' for c in prune_chars} for record in records: record.seq = Seq(str(record.seq).translate(translation_table), record.seq.alphabet) ...
def dashes_cleanup(records, prune_chars='.:?~')
Take an alignment and convert any undesirable characters such as ? or ~ to -.
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logging.info('Applying _deduplicate_sequences generator: ' 'removing any duplicate records with identical sequences.') checksum_sequences = collections.defaultdict(list) for record in records: checksum = seguid(record.seq) sequences = checksum_sequences[checksum] ...
def deduplicate_sequences(records, out_file)
Remove any duplicate records with identical sequences, keep the first instance seen and discard additional occurences.
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logging.info('Applying _deduplicate_taxa generator: ' + \ 'removing any duplicate records with identical IDs.') taxa = set() for record in records: # Default to full ID, split if | is found. taxid = record.id if '|' in record.id: try: ...
def deduplicate_taxa(records)
Remove any duplicate records with identical IDs, keep the first instance seen and discard additional occurences.
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logging.info('Applying _first_name_capture generator: ' 'making sure ID only contains the first whitespace-delimited ' 'word.') whitespace = re.compile(r'\s+') for record in records: if whitespace.search(record.description): yield SeqRecord(record.s...
def first_name_capture(records)
Take only the first whitespace-delimited word as the name of the sequence. Essentially removes any extra text from the sequence's description.
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ids = set(i.strip() for i in handle) for record in records: if record.id.strip() in ids: yield record
def include_from_file(records, handle)
Filter the records, keeping only sequences whose ID is contained in the handle.
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# Check arguments if end <= start: raise ValueError("start of slice must precede end ({0} !> {1})".format( end, start)) for sequence in sequences: seq = sequence.seq start_gap = gap_char * start end_gap = gap_char * (len(seq) - end) seq = Seq(start_g...
def isolate_region(sequences, start, end, gap_char='-')
Replace regions before and after start:end with gap chars
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for record in records: # Generate a set of indices to remove drop = set(i for slice in slices for i in range(*slice.indices(len(record)))) keep = [i not in drop for i in range(len(record))] record.seq = Seq(''.join(itertools.compress(record.seq, keep)), record...
def drop_columns(records, slices)
Drop all columns present in ``slices`` from records
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with _record_buffer(records) as r: try: record = next(i for i in r() if i.id == record_id) except StopIteration: raise ValueError("Record with id {0} not found.".format(record_id)) new_slices = _update_slices(record, slices) for record in multi_cut_seque...
def cut_sequences_relative(records, slices, record_id)
Cuts records to slices, indexed by non-gap positions in record_id
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for record in records: record_indices = list(range(len(record))) keep_indices = reduce(lambda i, s: i - frozenset(record_indices[s]), slices, frozenset(record_indices)) seq = ''.join(b if i in keep_indices else '-' for i, b in enumerat...
def multi_mask_sequences(records, slices)
Replace characters sliced by slices with gap characters.
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for record in records: if not all(c == '-' for c in str(record.seq)): yield record
def prune_empty(records)
Remove any sequences which are entirely gaps ('-')
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# Copy the annotations over for k, v in list(old_record.annotations.items()): # Trim if appropriate if isinstance(v, (tuple, list)) and len(v) == len(old_record): assert len(v) == len(old_record) v = v[::-1] new_record.annotations[k] = v # Letter annotat...
def _reverse_annotations(old_record, new_record)
Copy annotations form old_record to new_record, reversing any lists / tuples / strings.
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logging.info('Applying _reverse_sequences generator: ' 'reversing the order of sites in sequences.') for record in records: rev_record = SeqRecord(record.seq[::-1], id=record.id, name=record.name, description=record.desc...
def reverse_sequences(records)
Reverse the order of sites in sequences.
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logging.info('Applying _reverse_complement_sequences generator: ' 'transforming sequences into reverse complements.') for record in records: rev_record = SeqRecord(record.seq.reverse_complement(), id=record.id, name=record.name, ...
def reverse_complement_sequences(records)
Transform sequences into reverse complements.
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logging.info('Applying _ungap_sequences generator: removing all gap characters') for record in records: yield ungap_all(record, gap_chars)
def ungap_sequences(records, gap_chars=GAP_TABLE)
Remove gaps from sequences, given an alignment.
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old_id = record.id record.id = new_id # At least for FASTA, record ID starts the description record.description = re.sub('^' + re.escape(old_id), new_id, record.description) return record
def _update_id(record, new_id)
Update a record id to new_id, also modifying the ID in record.description
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logging.info('Applying _name_append_suffix generator: ' 'Appending suffix ' + suffix + ' to all ' 'sequence IDs.') for record in records: new_id = record.id + suffix _update_id(record, new_id) yield record
def name_append_suffix(records, suffix)
Given a set of sequences, append a suffix for each sequence's name.
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logging.info('Applying _name_insert_prefix generator: ' 'Inserting prefix ' + prefix + ' for all ' 'sequence IDs.') for record in records: new_id = prefix + record.id _update_id(record, new_id) yield record
def name_insert_prefix(records, prefix)
Given a set of sequences, insert a prefix for each sequence's name.
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logging.info('Applying _name_include generator: ' 'including only IDs matching ' + filter_regex + ' in results.') regex = re.compile(filter_regex) for record in records: if regex.search(record.id) or regex.search(record.description): yield record
def name_include(records, filter_regex)
Given a set of sequences, filter out any sequences with names that do not match the specified regular expression. Ignore case.
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regex = re.compile(search_regex) for record in records: maybe_id = record.description.split(None, 1)[0] if maybe_id == record.id: record.description = regex.sub(replace_pattern, record.description) record.id = record.description.split(None, 1)[0] else: ...
def name_replace(records, search_regex, replace_pattern)
Given a set of sequences, replace all occurrences of search_regex with replace_pattern. Ignore case. If the ID and the first word of the description match, assume the description is FASTA-like and apply the transform to the entire description, then set the ID from the first word. If the ID and the ...
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regex = re.compile(filter_regex) for record in records: if regex.search(str(record.seq)): yield record
def seq_include(records, filter_regex)
Filter any sequences who's seq does not match the filter. Ignore case.
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