task stringlengths 34 71 | input stringlengths 8 997 | output stringlengths 1 255 | options list | pageTitle stringlengths 18 37 | outputColName stringlengths 1 30 | url stringlengths 39 45 | wdcFile stringlengths 71 74 |
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5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] Transparent [Answer] | light passes through freely | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] translucent [Answer] | light passes through and scatteres (some is absorbed or reflected) | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] opaque [Answer] | no light can pass through, all light is absorbed or reflected | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] shadows [Answer] | bigger if object is closer to light sorce, smaller if object is farther from light sorce | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] normal [Answer] | line drawn at right angle to a soldid barrier | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] incident ray [Answer] | incoming light ray | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] reflected ray [Answer] | light ray that bounces off | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] angle of incidence [Answer] | angle between incident ray and the normal, labelled with an i | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] angle of reflection [Answer] | angle bvetween reflected ray and the normal, labelled with an r | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] law of reflection [Answer] | the angle of reflection equals the angle of incidence | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] refraction [Answer] | bending of a wave when it passes from one medium to another | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] light passing through air to water (refraction)bends ___________the normal [Answer] | slows down and bends toward | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] light passing through water to air (refraction) bends ___________ the normal [Answer] | speeds up and bends away from | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] angle of refraction [Answer] | angle between the refracted ray and the normal, labelled with an R | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] when you look at an object under water, it appears to be _______ where it really is [Answer] | above | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
5d3ad7e4_lashcards_about_Science_exam_5__Answer | [Question] light can also be refracted in air, resulting in _________ [Answer] | mirages | [] | Flashcards about Science exam 5 | Answer | http://www.studystack.com/flashcard-176929 | 29/1438042990123.20_20150728002310-00208-ip-10-236-191-2_723934359_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] Recessive trait [Question] | A genetic trait that is HIDDEN by a dominant trait. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] Dominant trait [Question] | A genetic trait that expresses itself more STRONGLY than another trait. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] Genetics [Question] | The SCIENCE of heredity | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] trait [Question] | CHARACTERISTICS such as leaf shape or eye color that are passed from parent to offspring. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] gene [Question] | The section of DNA that codes for one specific trait. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] DNA [Question] | Consists of strands of molecules that code and store genetic information; located in the nucleus of the cell. It's like a blueprint for your body. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] Allele [Question] | One version of a gene | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] Watson and Crick [Question] | Scientists who discovered the structure of the DNA molecule (notice this is PLURAL--it will be more than one name) | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] double helix [Question] | The SHAPE of a DNA molecule. It looks like a twisted ladder. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] heredity (verb form--inherit)(My labrador inherited his yellow fur from his mother and father.) [Question] | The passing of traits from parents to offspring. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] species(Dogs are a species of mammals. Dogs can breed and produce more dogs.) [Question] | A group of similar organisms that can reproduce together and produce fertile offspring. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] ethics [Question] | the processing of thinking & discussing ISSUES OF RIGHT & WRONG | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] offspring [Question] | The YOUNG of a person, animal, or plant | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] sexual reproduction [Question] | A form of reproduction that requires TWO PARENTS and results in offspring who are genetically DIFFERENT from their parents. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] hybrid [Question] | An organism with dominant allele AND a recessive allele for a trait. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
b167cd37_cards_about_Genetics_7th_Grade__Question | [Answer] clone [Question] | An organism that is genetically IDENTICAL to the organism from which is was produced. | [] | Flashcards about Genetics 7th Grade | Question | http://www.studystack.com/flashcard-230701 | 29/1438042990123.20_20150728002310-00227-ip-10-236-191-2_736172537_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] Proceeding from contrition [Question] | Contrite | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] Someone who suffers death [Question] | Martyr | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] Venchful [Question] | Vindiction | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] to come to the end [Question] | Succumb | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] to overcome by force [Question] | Surmount | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] cant be replaced by anything [Question] | Irreplaceable | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] lack of proper respect [Question] | Irrevent | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] not possible to revoke [Question] | Irrevocable | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] not reparable [Question] | Irreparable | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] not trust worthy [Question] | Irresponsible | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] a close circle [Question] | Circurt | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] to expand [Question] | Circumscribe | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] to manage to get around [Question] | Circumvent | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] indenect corse [Question] | Ciruitous | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] wordy [Question] | Circumlocution | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] distinguished by rank or title [Question] | Noble | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] respect or reverence paid or rendered [Question] | Homage | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] to deliver an oration [Question] | Orate | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] to clear [Question] | Exonerate | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
5dd77604_ashcards_about_Review_For_Quiz__Question | [Answer] the laying on of something as a burden or obligation [Question] | Imposition | [] | Flashcards about Review For Quiz | Question | http://www.studystack.com/flashcard-521310 | 29/1438042990123.20_20150728002310-00224-ip-10-236-191-2_723193654_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What happens when PLC is activated? [Answer] | Causes an influx of calcium ions from the ER to the cytoplasm | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Calcium is a direct trigger for? [Answer] | exocytosis of cortical granules. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Cortical Granules contain? [Answer] | proteases that cut the protein tethering the vitelline envelope to the egg and muco-polysaccharide which produces an osmotic gradient, causing water to rush into this space. They also contain peroxidase which uses H2O2 to harden the envelope. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] calcium ionophore [Answer] | Causes an artificial influx of Ca ions which induces exocytosis of the c.g.'s and other fert. events and produces activation w/out fertilization. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] MAPK stands for [Answer] | Mitrogen Activated Protein Kinase | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] MAPK keeps [Answer] | oocytes in an arrested state until fertilization. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] MAPK is turned on/off by.. [Answer] | phosphorylation/de-phosphorylation | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What AA's must MAPK be phosphorylated at? [Answer] | Thr 202 and Tyr 204 | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Ionophore [Answer] | Is a compound that facilitates duffusion of an ion across a cell membrane. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] A23187 [Answer] | Is a lipid soluble mol'c that enters the membrane and allows calcium to move across the membrane down it's conc. gradient. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] DMSO stands for [Answer] | dimethylsulfoxide. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] The key to a correctly controlled experiment is [Answer] | For any experimental condition, you should have another experimental cond. where not more than one variable changes btwn the 2 samples. Keeping sample sizes uniform and having enough replicates to make sure affects are not due to random variation in data. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] MAPK lysis buffer with inhibitors [Answer] | lysis buffer lyses the cell membrane. The inhibitor contains protease inhibitors that stop MAKP from getting degraded. The others are phosphatase inhibitors that prevent dephosphorylation by stray phosphatases after the cells are lysed. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] After MAPK is treated with lysis buffer and inhibitor cocktail, it is centrifuged. Where will the MAPK be? [Answer] | The supernatant. The pellet will contain nuclei and insoluble cell debris. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] SDS-PAGE stands for [Answer] | sodium dodesyl sulfate | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What is the role of SDS in electrophoresis? [Answer] | To eliminate the charge by coating all proteins with a net negative charge. It will also denature the proteins so that shape will not factor migration rate. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] mercaptoehtanol [Answer] | is a reducing agent that breaks disulfide linkages (covalent bonds) in a protein | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] heat [Answer] | will remove any remaining folding not accomplished from SDS or mercaptoethanol. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] The pre-cast gels contained 10% acrylamide. What is the fractioning range? [Answer] | 30-100kDa | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] The purpose of the stacking gel is? [Answer] | To compress all of the proteins in the sample into a thin band so that they enter the running gel simultaneously. This way proteins of the same MW will be present in discrete bands. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Want antibody will probe the sea urchin samples during SDS-PAGE gel? [Answer] | anti-pMAPK and anti-tMAPK | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Why use Western Blotting? [Answer] | It is used when you need to specifically detect one protein in a mix of proteins. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] How are the proteins transfered from the polyacrylimide gel to NC membrane? [Answer] | electrophoretically | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Factors that influence protein transfer [Answer] | The buffer, the membrane, voltage, time and charge. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Why is voltage important when transferring a protein from polyacrylimide gel to a hydrophobic membrane like NC or PVDF? [Answer] | If the current is too high, low MW proteins could migrate too fast and not get a chance to bind to the membrane. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What does the blocking buffer consist of and what is it's purpose? [Answer] | It consists of TBST+5%BSA. It's purpose is to bind to unoccupied sites on the NC filter so antibodies won't stick non-specifically. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What does TBST stand for? [Answer] | Tris-Buffered Saline and Tween. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What does Tween do? [Answer] | Reduces non-specific binding of proteins to the antigens. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] antigen [Answer] | The substances that antibodies bind to. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] IgG [Answer] | Is a tetramer composed of 2 heavy chains and 2 light chains that are held together by disulfide bridges. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Constant Region [Answer] | The AA sequence in the base of the "Y". Does not vary for a given species. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Variable Region [Answer] | The AA sequence at the ends of the "Y". Does very and is the part of the IgG that bind to antigen. The diff AA seq. of the var. reg's in diff. IgG mol'cs creates spec. binding sites that bind to diff antigens. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What does it mean to raise antibodies against antibodies? [Answer] | Ab's raised against Ab's are secondary Ab's. Ex. using a goat to raise Ab's agains rabbit IgG's. Sec. Ab's bind to the constant region of primary antibodies. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] The primary antibody is what binds to the... [Answer] | to the protein of interest. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] The secondary allows you to... [Answer] | visualize the bound primary protein by producing a signal b/c it usually has a tag or peroxidase enzyme covalently linked to the con. reg. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] How is the secondary Ab created? [Answer] | An animal is injected with the cons. reg. portion of IgG from the animal in which the primary Ab is raised. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] How are Ab's helpful in biochem? [Answer] | They can be used to help determine where in a cell an antigen may be concentrated. Whether a cell express a certain mol'c on it's surface, whether a mol'c is present in a sample, or to quantify the amount of antigen in sample and for purifying an antigen | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Is it possible for an antibody to bind an antigen other than the one for which it was created? [Answer] | Yes, some mol'cs have sim. structures. This is called cross reacting. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] ELISA stands for [Answer] | Enzyme Linked Immuno Sorbent Assay | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What does an ELISA accomplish? [Answer] | It is used to detect antigen-antibody complex with the help of an enzyme that converts a substrate to a colored product. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What antigen is detected by ELISA? [Answer] | inositol monophosphate, which is the breakdown product of a critical second messenger that is produced immediately after sperm bind (before Ca influx) | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What are the three types of ELISA tests? [Answer] | antibody capture assay, two-site capture assay and competition assay. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What is the trigger for the cytoplasmic Ca influx in fertilized sea urchin eggs? [Answer] | The activation of PLC and the subsequent production of IP3. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What is PLC? [Answer] | It is a lipase that hydrolysis reactions on lipids to cut them into smaller pieces. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What does PLC do? [Answer] | It catalyzes the cleavage of the phosphorylated head group from a phospholipid called PIP2. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] Where is PIP2 located? [Answer] | In the inner leaflet of the PM. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] When PLC cleaves the IP3 head from PIP2, what is produced? [Answer] | Large quantities of IP3 and DAG. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
36a9ee01_Flashcards_about_MAPK__Answer | [Question] What happens to IP3 when it is cleaved? [Answer] | It diffuses throughout the cytoplasm where it interacts with specific receptors. Receptor binding on the ER trigger Ca channels to open producing the Ca influx. | [] | Flashcards about MAPK | Answer | http://www.studystack.com/flashcard-412769 | 29/1438042987628.47_20150728002307-00274-ip-10-236-191-2_734658562_3.json |
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