Lal Claude Opus 4.6 commited on
Commit ·
06d49b4
1
Parent(s): 2506e10
Add row counts, fix loading code bug
Browse files- Add statistics table with row counts (peaks: 83K, fragments: 58M)
- Fix variable name bug in peaks loading (peak_file -> peak_path)
- Remove grelu.io.bed import (use pandas directly)
Co-Authored-By: Claude Opus 4.6 <noreply@anthropic.com>
README.md
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@@ -17,35 +17,40 @@ This dataset contains pseudobulk scATAC-seq data for human microglia, derived fr
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## Dataset Structure
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The dataset is divided into two
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###
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- `name`: Peak identifier.
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- `score`: Integer score for display.
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- `strand`: Orientation.
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- `signalValue`: Measurement of overall enrichment.
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- `pValue`: Statistical significance (-log10).
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- `qValue`: False discovery rate (-log10).
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- `peak`: Point-source (summit) relative to start.
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###
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Standard BED6 format representing individual ATAC-seq fragments.
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- `chrom`: Chromosome
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- `start`: Start position
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- `end`: End position
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- `source`: Sequencing run identifier (e.g., `SRR11442505`)
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- `score`: Placeholder (0)
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- `strand`: Orientation
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## Usage
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```python
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from huggingface_hub import hf_hub_download
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import grelu.io.bed
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import pandas as pd
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peak_path = hf_hub_download(
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repo_type="dataset",
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filename="peak_file.narrowPeak"
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peaks =
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frag_path = hf_hub_download(
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repo_id="Genentech/microglia-scatac-tutorial-data",
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repo_type="dataset",
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filename="fragment_file.bed"
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fragments = pd.read_csv(frag_path, sep='\t', header=None,
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names=['chrom', 'start', 'end', 'source', 'score', 'strand'])
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```
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## Dataset Structure
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The dataset is divided into two files: peaks and fragments.
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### Statistics
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| File | Rows | Description |
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|------|------|-------------|
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| `peak_file.narrowPeak` | 83,320 | Called ATAC-seq peaks |
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| `fragment_file.bed` | 57,919,016 | Individual ATAC-seq fragments |
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### 1. Peaks file (`peak_file.narrowPeak`)
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Standard ENCODE narrowPeak format (tab-separated, no header).
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- `chrom`: Chromosome / Contig name
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- `start`: 0-based start position
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- `end`: End position
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- `name`: Peak identifier
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- `score`: Integer score for display
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- `strand`: Orientation
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- `signalValue`: Measurement of overall enrichment
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- `pValue`: Statistical significance (-log10)
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- `qValue`: False discovery rate (-log10)
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- `peak`: Point-source (summit) relative to start
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### 2. Fragments file (`fragment_file.bed`)
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Standard BED6 format representing individual ATAC-seq fragments.
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- `chrom`: Chromosome
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- `start`: Start position
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- `end`: End position
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- `source`: Sequencing run identifier (e.g., `SRR11442505`)
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- `score`: Placeholder (0)
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- `strand`: Orientation
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## Usage
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```python
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from huggingface_hub import hf_hub_download
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import pandas as pd
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peak_path = hf_hub_download(
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repo_type="dataset",
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filename="peak_file.narrowPeak"
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)
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peaks = pd.read_csv(peak_path, sep='\t', header=None)
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frag_path = hf_hub_download(
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repo_id="Genentech/microglia-scatac-tutorial-data",
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repo_type="dataset",
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filename="fragment_file.bed"
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)
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fragments = pd.read_csv(frag_path, sep='\t', header=None,
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names=['chrom', 'start', 'end', 'source', 'score', 'strand'])
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```
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