Upload 3 files

app.py

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+from fastapi import FastAPI
+from pydantic import BaseModel
+from mutation_analyzer import find_mutations
+
+app = FastAPI()
+
+class DNAInput(BaseModel):
+    reference: str
+    sample: str
+
+@app.post("/analyze")
+def analyze_dna(data: DNAInput):
+    reference = data.reference.upper()
+    sample = data.sample.upper()
+
+    mutations = find_mutations(reference, sample)
+
+    message = "No mutation found. Sequence matches reference."
+    if len(mutations) > 0:
+        message = f"Mutation found! Total mutations detected: {len(mutations)}"
+
+    return {
+        "message": message,
+        "total_mutations": len(mutations),
+        "mutations": mutations
+    }

mutation_analyzer.py

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+import sys
+import json
+
+def find_mutations(reference, sample):
+    mutations = []
+
+    i, j = 0, 0
+    pos = 1  # 1-based position for biology
+
+    while i < len(reference) and j < len(sample):
+        if reference[i] == sample[j]:
+            i += 1
+            j += 1
+            pos += 1
+        else:
+            # Substitution
+            if i + 1 < len(reference) and j + 1 < len(sample) and reference[i+1] == sample[j+1]:
+                mutations.append({
+                    "type": "substitution",
+                    "position": pos,
+                    "reference": reference[i],
+                    "observed": sample[j]
+                })
+                i += 1
+                j += 1
+                pos += 1
+            # Insertion
+            elif j + 1 < len(sample) and reference[i] == sample[j+1]:
+                mutations.append({
+                    "type": "insertion",
+                    "position": pos,
+                    "inserted": sample[j]
+                })
+                j += 1
+                pos += 1
+            # Deletion
+            elif i + 1 < len(reference) and reference[i+1] == sample[j]:
+                mutations.append({
+                    "type": "deletion",
+                    "position": pos,
+                    "deleted": reference[i]
+                })
+                i += 1
+                pos += 1
+            else:
+                mutations.append({
+                    "type": "substitution",
+                    "position": pos,
+                    "reference": reference[i],
+                    "observed": sample[j]
+                })
+                i += 1
+                j += 1
+                pos += 1
+
+    while j < len(sample):
+        mutations.append({
+            "type": "insertion",
+            "position": pos,
+            "inserted": sample[j]
+        })
+        j += 1
+        pos += 1
+
+    while i < len(reference):
+        mutations.append({
+            "type": "deletion",
+            "position": pos,
+            "deleted": reference[i]
+        })
+        i += 1
+        pos += 1
+
+    return mutations
+
+
+if __name__ == "__main__":
+    data = json.loads(sys.stdin.read())
+
+    reference = data["reference"].upper()
+    sample = data["sample"].upper()
+
+    result = find_mutations(reference, sample)
+
+    message = "No mutation found. Sequence matches reference."
+    if len(result) > 0:
+        message = f"Mutation found! Total mutations detected: {len(result)}"
+
+    print(json.dumps({
+        "message": message,
+        "total_mutations": len(result),
+        "mutations": result
+    }))

requirements.txt

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+fastapi
+uvicorn