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| """ |
| This dataset contains example data for running through the multiplexed imaging data pipeline in |
| Ark Analysis: https://github.com/angelolab/ark-analysis. |
| |
| |
| Dataset Fov renaming: |
| |
| TMA2_R8C3 -> fov0 |
| TMA6_R4C5 -> fov1 |
| TMA7_R5C4 -> fov2 |
| TMA10_R7C3 -> fov3 |
| TMA11_R9C6 -> fov4 |
| TMA13_R8C5 -> fov5 |
| TMA17_R9C2 -> fov6 |
| TMA18_R9C2 -> fov7 |
| TMA21_R2C5 -> fov8 |
| TMA21_R12C6 -> fov9 |
| TMA24_R9C1 -> fov10 |
| |
| """ |
|
|
| import datasets |
| import pathlib |
|
|
| |
| _CITATION = """\ |
| @InProceedings{huggingface:dataset, |
| title = {Ark Analysis Example Dataset}, |
| author={Angelo Lab}, |
| year={2022} |
| } |
| """ |
|
|
| |
| |
| _DESCRIPTION = """\ |
| This dataset contains 11 Field of Views (FOVs), each with 22 channels. |
| """ |
|
|
| _HOMEPAGE = "https://github.com/angelolab/ark-analysis" |
|
|
| _LICENSE = "https://github.com/angelolab/ark-analysis/blob/main/LICENSE" |
|
|
| |
| |
|
|
| _URL_DATA = { |
| "image_data": "./data/image_data.zip", |
| "cell_table": "./data/segmentation/cell_table.zip", |
| "deepcell_output": "./data/segmentation/deepcell_output.zip", |
| "example_pixel_output_dir": "./data/pixie/example_pixel_output_dir.zip", |
| "example_cell_output_dir": "./data/pixie/example_cell_output_dir.zip", |
| "spatial_lda": "./data/spatial_analysis/spatial_lda.zip", |
| "post_clustering": "./data/post_clustering.zip" |
| } |
|
|
| _URL_DATASET_CONFIGS = { |
| "segment_image_data": {"image_data": _URL_DATA["image_data"]}, |
| "cluster_pixels": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| "deepcell_output": _URL_DATA["deepcell_output"], |
| }, |
| "cluster_cells": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| "deepcell_output": _URL_DATA["deepcell_output"], |
| "example_pixel_output_dir": _URL_DATA["example_pixel_output_dir"], |
| }, |
| "post_clustering": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| "deepcell_output": _URL_DATA["deepcell_output"], |
| "example_cell_output_dir": _URL_DATA["example_cell_output_dir"], |
| }, |
| "fiber_segmentation": { |
| "image_data": _URL_DATA["image_data"], |
| }, |
| "LDA_preprocessing": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| }, |
| "LDA_training_inference": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| "spatial_lda": _URL_DATA["spatial_lda"], |
| }, |
| "neighborhood_analysis": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| "deepcell_output": _URL_DATA["deepcell_output"], |
| }, |
| "pairwise_spatial_enrichment": { |
| "image_data": _URL_DATA["image_data"], |
| "cell_table": _URL_DATA["cell_table"], |
| "deepcell_output": _URL_DATA["deepcell_output"], |
| "post_clustering": _URL_DATA["post_clustering"], |
| } |
| } |
|
|
|
|
| |
| class ArkExample(datasets.GeneratorBasedBuilder): |
| """The Dataset consists of 11 FOVs""" |
|
|
| VERSION = datasets.Version("0.0.4") |
|
|
| |
| BUILDER_CONFIGS = [ |
| datasets.BuilderConfig( |
| name="segment_image_data", |
| version=VERSION, |
| description="This configuration contains data used by notebook 1 - Segment Image Data.", |
| ), |
| datasets.BuilderConfig( |
| name="cluster_pixels", |
| version=VERSION, |
| description="This configuration contains data used by notebook 2 - Pixel Clustering (Pixie Pipeline #1).", |
| ), |
| datasets.BuilderConfig( |
| name="cluster_cells", |
| version=VERSION, |
| description="This configuration contains data used by notebook 3 - Cell Clustering (Pixie Pipeline #2).", |
| ), |
| datasets.BuilderConfig( |
| name="post_clustering", |
| version=VERSION, |
| description="This configuration contains data used by notebook 4 - Post Clustering.", |
| ), |
| datasets.BuilderConfig( |
| name="fiber_segmentation", |
| version=VERSION, |
| description="This configuration contains data used by the Fiber Segmentation Notebook.", |
| ), |
| datasets.BuilderConfig( |
| name="LDA_preprocessing", |
| version=VERSION, |
| description="This configuration contains data used by the Spatial LDA - Preprocessing Notebook." |
| ), |
| datasets.BuilderConfig( |
| name="LDA_training_inference", |
| version=VERSION, |
| description="This configuration contains data used by the Spatial LDA - Training and Inference Notebook." |
| ), |
| datasets.BuilderConfig( |
| name="neighborhood_analysis", |
| version=VERSION, |
| description="This configuration contains data used by the Neighborhood Analysis Notebook." |
| ), |
| datasets.BuilderConfig( |
| name="pairwise_spatial_enrichment", |
| version=VERSION, |
| description="This configuration contains data used by the Pairwise Spatial Enrichment Notebook." |
| ) |
| ] |
|
|
| def _info(self): |
| |
| if self.config.name in [ |
| "segment_image_data", |
| "cluster_pixels", |
| "cluster_cells", |
| "post_clustering", |
| "fiber_segmentation", |
| "LDA_preprocessing", |
| "LDA_training_inference", |
| "neighborhood_analysis", |
| "pairwise_spatial_enrichment", |
| ]: |
| features = datasets.Features( |
| {f: datasets.Value("string") for f in _URL_DATASET_CONFIGS[self.config.name].keys()} |
| ) |
| else: |
| ValueError(f"Dataset name is incorrect, options include {list(_URL_DATASET_CONFIGS.keys())}") |
| return datasets.DatasetInfo( |
| |
| description=_DESCRIPTION, |
| |
| features=features, |
| |
| |
| |
| |
| homepage=_HOMEPAGE, |
| |
| license=_LICENSE, |
| |
| citation=_CITATION, |
| ) |
|
|
| def _split_generators(self, dl_manager): |
| |
| urls = _URL_DATASET_CONFIGS[self.config.name] |
| data_dirs = {} |
| for data_name, url in urls.items(): |
| dl_path = pathlib.Path(dl_manager.download_and_extract(url)) |
| data_dirs[data_name] = dl_path |
|
|
| return [ |
| datasets.SplitGenerator( |
| name=self.config.name, |
| |
| gen_kwargs={"dataset_paths": data_dirs}, |
| ), |
| ] |
|
|
| |
| def _generate_examples(self, dataset_paths): |
| yield self.config.name, dataset_paths |
|
|