| {"qid": "ExpVid_4149", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Cover 2/3 of conductive side of FTO coated glass slide with semi-transparent acrylic adhesive tape\n2. Coat uncovered areas of slide with zinc powder\n3. Pour 2M hydrochloric acid solution in distilled water over slide to etch substrate\n4. Wipe residue from exposed slide sections using cotton swab\n5. Rinse etched FTO substrate with distilled water\n6. Remove tape from substrate\n7. Wash etched surface in 2% w/v alkaline liquid detergent concentrate solution\n8. Sonicate FTO substrate for 10 minutes in acetone bath\n9. Sonicate FTO substrate for 10 minutes in isopropanol bath\n10. Treat FTO substrate in oxygen plasma cleaner for 15 minutes\n11. Place etched and cleaned FTO substrate on hot plate at 450°C\n12. Cover contact area with precut glass slide\n13. Allow substrate to heat to 450°C\n14. Mix 0.6 mL TAA with 7 mL isopropanol\n15. Apply TAA solution to substrate via spray pyrolysis using air carrier gas at 450°C\n16. Maintain sample at 450°C for 30 minutes\n17. Cool sample to room temperature\n18. Remove glass cover after substrate cooling\n19. Dilute 30 nanomolar titanium oxide paste with ethanol to 2:7 weight ratio\n20. Sonicate suspension for 30 minutes\n21. Spin coat sample with suspension for 30 seconds at 5,000 RPM with 2,000 RPM/s ramp rate\n22. Anneal titanium film at 500°C for 30 minutes to form mesoporous titanium oxide film\n23. Immerse sample in 40 millimolar titanium chloride solution in distilled water\n24. Treat sample at 70°C for 20 minutes\n25. Anneal titanium chloride-treated sample at 450°C for 30 minutes\n26. Transfer sample to nitrogen-filled glove box with <1% humidity\n27. Add 1 milliliter of DMF to 553 milligrams of lead iodide\n28. Heat mixture to 80 degrees Celsius while stirring continuously\n29. Continue heating until lead iodide dissolves\n30. Prepare 0.02 molar solution of monovalent cation halide in lead iodide solution\n31. Apply 80 microliters of monovalent cation hali\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "40, 41, 42, 43, 44, 45, 46, 47", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/55307/clip_5.mp4"}} |
| {"qid": "ExpVid_4108", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Dissolve 2.6 grams of P nipam in 2 milliliters of 60% toluene and 40% hexane solution\n2. Heat mixture to 60 degrees Celsius for 10 minutes\n3. Stir mixture until P nipam is dissolved\n4. Cut filter paper into 60 millimeter diameter circle\n5. Place filter paper in Buchner funnel\n6. Filter solution through Buchner funnel into pre-weighed glass beaker\n7. Keep beaker and contents in bell vacuum overnight\n8. Weigh beaker with P nipam\n9. Add isopropyl alcohol to P nipam\n10. Create 50:50 weight/weight solution\n11. Place two milliliters of solution on tissue culture plate surface\n12. Coat plate for five minutes under UV light\n13. Wash cells with two milliliters of warm PBS\n14. Add three milliliters of trypsin or cleaving dissociating solution to cells\n15. Incubate for five minutes\n16. Add 3 mL of culture media or PBS containing 10% FBS to cells\n17. Collect cells in conical tube\n18. Count cells\n19. Centrifuge cells at 1000 RPM for 5 minutes\n20. Aspirate supernatant\n21. Resuspend cells in growth media\n22. Plate cell suspension on 35mm thermosensitive plate at concentration for 100% confluence\n23. Incubate plate at 37°C overnight\n24. Collect endothelial cells\n25. Disperse cells into single-cell solution\n26. Deactivate trypsin enzyme with equal amount of soybean trypsin inhibitor or 10% FBS in PBS\n27. Collect cells in 15 milliliter conical tube\n28. Count cells with hemocytometer and calculate volume needed for patch dimensions\n29. Extract 2 million endothelial cells\n30. Place extracted cells in new 15 milliliter conical tube\n31. Centrifuge cells for five minutes\n32. Aspirate supernatant, leaving cell pellet in conical tube\n33. Mix high aurin and liquid gelatin in 1:1 ratio\n34. Transfer 80% of final hydrogel-gelatin volume to conical tube containing endothelial cell pellet and resuspend cells\n35. Transfer suspended cells into fibrous matrix\n36. Add 20% of total final volume of crosslinker\n37\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "33, 34, 35, 36, 37", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/51044/clip_6.mp4"}} |
| {"qid": "ExpVid_7583", "question": "The main conclusion is that a standardized, repeatable ______ protocol enables quality assessment of Taiwanese green propolis by quantifying ______ and linking their yield to solvent concentration and antibacterial potency (measured by ______) against ______.", "options": null, "answer": "ethanol-extraction–HPLC | propolins (C, D, F, G) | MIC/MBC | Staphylococcus aureus", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/58743_.mp4"}} |
| {"qid": "ExpVid_7438", "question": "The study concludes that transforming potato with ____ rapidly produces transgenic ____ whose promoter-driven GUS pattern matches results from ____, showing blue staining in the ____ (and at lateral root emergence), establishing a fast platform for root-specific studies.", "options": null, "answer": "Agrobacterium rhizogenes | hairy roots | Agrobacterium tumefaciens | endodermis and exodermis", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/59119_.mp4"}} |
| {"qid": "ExpVid_6691", "question": "Given the following step list，which step was not performed in the video?\n1. Thaw plastic tube containing aliquot of frozen suspension of small lipid vesicles at room temperature\n2. Vortex tube four times for 1-2 seconds using vortex mixer at maximum speed\n3. Place 5 microliters of small lipid vesicle suspension onto surface of uncleaned glass coverslip to form small round droplet\n4. Place glass coverslip in vacuum desiccator for 20 minutes\n5. Store dried lipid film at room temperature for 4 minutes", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/57789/clip_4_removed_step_4.mp4"}} |
| {"qid": "ExpVid_4805", "question": "What is the correct sequence of steps for pellet aliquoting and weighing?", "options": ["A. 1. Set out three pre-weighed 50 milliliter conical tubes (cold)\n2. Aliquot 10 milliliters of pellet suspension into each tube using serological pipette filler with sterile pipette\n3. Reweigh tubes using analytical balance and report final pellet weight for each tube\n4. Centrifuge tubes at 5,000 g and 10 degrees Celsius for 10 minutes with appropriate balancing\n5. Pour out and dispose of supernatants\n6. Wipe inside of each tube and cap with clean tissue to remove excess buffer while avoiding pellet contact", "B. 1. Set out three pre-weighed 50 milliliter conical tubes (cold)\n2. Aliquot 10 milliliters of pellet suspension into each tube using serological pipette filler with sterile pipette\n3. Centrifuge tubes at 5,000 g and 10 degrees Celsius for 10 minutes with appropriate balancing\n4. Pour out and dispose of supernatants\n5. Wipe inside of each tube and cap with clean tissue to remove excess buffer while avoiding pellet contact\n6. Reweigh tubes using analytical balance and report final pellet weight for each tube", "C. 1. Aliquot 10 milliliters of pellet suspension into each tube using serological pipette filler with sterile pipette\n2. Set out three pre-weighed 50 milliliter conical tubes (cold)\n3. Centrifuge tubes at 5,000 g and 10 degrees Celsius for 10 minutes with appropriate balancing\n4. Pour out and dispose of supernatants\n5. Wipe inside of each tube and cap with clean tissue to remove excess buffer while avoiding pellet contact\n6. Reweigh tubes using analytical balance and report final pellet weight for each tube", "D. 1. Set out three pre-weighed 50 milliliter conical tubes (cold)\n2. Aliquot 10 milliliters of pellet suspension into each tube using serological pipette filler with sterile pipette\n3. Centrifuge tubes at 5,000 g and 10 degrees Celsius for 10 minutes with appropriate balancing\n4. Wipe inside of each tube and cap with clean tissue to remove excess buffer while avoiding pellet contact\n5. Pour out and dispose of supernatants\n6. Reweigh tubes using analytical balance and report final pellet weight for each tube"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/58882/clip_4.mp4"}} |
| {"qid": "ExpVid_5787", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Place anesthetized mouse in supine position on sterile-draped surgery table\n2. Apply ocular lubricant to protect eyes from drying\n3. Shave abdominal hair for operation and leg hair for postoperative perfusion measurements using pen trimmer\n4. Clear shaved hair off surgical field\n5. Fixate upper and lower extremities with rubber bands and tacks\n6. Check anesthesia depth via toe pinch reflex and titrate anesthesia as necessary\n7. Perform respiratory pattern evaluation every 3-5 minutes to calibrate anesthesia level\n8. Perform midline laparotomy from lower sternal margin to pubis symphysis\n9. Dissect pubis fat pad to widen operative field\n10. Open celiotomy to access peritoneal contents\n11. Cover bowels with saline-soaked non-woven sponge\n12. Dissect perivascular fascia and adipose tissue from 1 cm proximal to aortic bifurcation to left iliac bifurcation using straight Dumont forceps and double-ended sharp cotton swabs\n13. Pass tip of angled forceps under left common iliac vascular bundle\n14. Gently spread forceps multiple times to mobilize vessels from retroperitoneal musculature\n15. Place two 4-0 silk sutures around isolated left common iliac arteriovenous bundle as ligatures\n16. Create single knot with each 4-0 silk tie\n17. Apply knots sequentially (proximally then distally)\n18. Rotate left iliac arteriovenous bundle clockwise using 4-0 silk suture strings as handles\n19. Fine-tune position to temporarily locate vein anterior to artery\n20. Make 1mm longitudinal venotomy using straight Vannas spring scissors\n21. Flush residual blood from venous lumen with 0.9% saline\n22. Place 10-0 nylon suture through posterior wall of vein\n23. Grab suture ends and apply gentle tension to separate anterior and posterior vessel walls\n24. Make elliptical incision (1.0 × 0.3 mm) using curved Vannas spring scissors\n25. Remove \nAnswer with the step number only.", "options": null, "answer": "11", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/63892/clip_2_prediction.mp4"}} |
| {"qid": "ExpVid_7518", "question": "This study demonstrates that _____ self-polymerization at _____ yields a universal, thickness-tunable adhesive coating on nanodiamonds that enhances dispersion and, without any additional reducing agent, reduces and immobilizes _____ to form _____ on the surface.", "options": null, "answer": "polydopamine (PDA) | pH 8.5 | diamine silver hydroxide ([Ag(NH3)2]+) | silver nanoparticles", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/58458_.mp4"}} |
| {"qid": "ExpVid_4427", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Place adult male mouse (≥8 weeks old) in supine position\n2. Disinfect ventral abdomen with 70% ethanol\n3. Make v-shaped incision in abdominal pelvic region using sterile scissors\n4. Pull epididymal fat pad using sterile forceps to locate testes\n5. Dissect testes using scissors\n6. Place harvested testes in sterile 100-mm Petri dish containing PBS\n7. Cut slit in tunica albuginea using fine-tipped scissors\n8. Tear tunica albuginea open using forceps\n9. Force seminiferous tubules out of tunica albuginea\n10. Transfer decapsulated seminiferous tubules to new Petri dish\n11. Add sterile PBS to cover bottom of Petri dish\n12. Gently separate tubules using forceps without tissue damage\n13. Tape dish onto dissecting microscope stage\n14. Turn on transillumination\n15. Move tubule bundles with fine forceps to observe light absorption and scatter patterns\n16. Lift tubule of interest using hooked-tip forceps\n17. Cut tubule segment using microdissection scissors\n18. Collect tubule segment using pipette in 10 microliters of PBS\n19. Place tubule segment onto microscope slide\n20. Press 20x20 mm cover glass onto tubule while avoiding excessive cell squashing\n21. Place prepared slide onto phase-contrast microscope stage\n22. Place filter paper onto edge of cover glass\n23. Use 40x microscope objective to verify cell stages\n24. Dip slide into liquid nitrogen for 10 seconds\n25. Use scalpel to flip cover glass off cells\n26. Place slide in 90% ethanol for 2-5 minutes\n27. Air dry slide\n28. Store slide at room temperature for up to several days\n29. Transfer segments of interest into 15-mL conical tube containing ice-cold PBS using pipette\n30. Remove PBS after allowing segments to sediment\n31. Add 10 mL fresh ice-cold PBS\n32. Mix sample by inversion\n33. Allow tubules to settle for several minutes\n34. Remove supernatant after settlement\n35. Fix tubules in 5 milliliters of paraformaldehyde for 5 hours at 20-30\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "1, 2, 3, 4, 5, 6", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61800/clip_1.mp4"}} |
| {"qid": "ExpVid_5747", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Document weight of human brain tissue\n2. Remove meninges with forceps under microscope\n3. Cut off white matter with scalpel\n4. Mince brain tissue with scalpel for approximately five minutes\n5. Transfer minced brain tissue to tissue grinder\n6. Add 30 milliliters of isolation buffer to tissue grinder\n7. Homogenize each sample with 100 strokes at 50 rpm\n8. Avoid stirring in air to prevent bubble formation\n9. Document time every 25 strokes and total time for 100 strokes\n10. Transfer homogenate to Dounce homogenizer on ice\n11. Homogenize suspension for 20 strokes while avoiding bubbles\n12. Distribute brain homogenate equally into four 50 mL centrifugation tubes\n13. Document total volume of homogenate\n14. Rinse pestle and homogenizer with 10 milliliters of isolation buffer\n15. Distribute isolation buffer into four centrifugation tubes\n16. Distribute 50 milliliters of density gradient buffer into centrifugation tubes\n17. Tightly close centrifuge tubes with caps\n18. Vigorously shake tubes to mix homogenate, density gradient medium, and buffer\n19. Centrifuge at 5,800xg for 15 minutes at 4°C\n20. Discard supernatant\n21. Resuspend each pellet in 2 milliliters of 1% BSA\n22. Filter suspension through 300 micrometer mesh\n23. Wash mesh with up to 50 milliliters of 1% BSA\n24. Discard mesh\n25. Distribute capillary filtrate over five 30 micrometer cell strain filters\n26. Wash each filter with 25 milliliters of 1% BSA\n27. Pour all filtrates over sixth filter\n28. Wash each filter with 50 milliliters of 1% BSA\n29. Retain cell strain filters containing capillaries\n30. Discard filtrate\n31. Turn filters upside down\n32. Wash capillaries with 50 milliliters of 1% BSA into 50 milliliter tubes\n33. Gently apply pressure with pipette tip across filter surface\n34. Move pipette tip across filter to wash off brain capillaries\n35. Ensure c\nAnswer with the step number only.", "options": null, "answer": "35", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/57346/clip_6_prediction.mp4"}} |
| {"qid": "ExpVid_6910", "question": "Given the following step list，which step was not performed in the video?\n1. Shave 1 cm along ventral surface of tail starting 1 cm from base\n2. Wipe shaved tail region with Betadine three times\n3. Secure mouse in stereotaxic frame\n4. Maintain mouse temperature at 37°C using thermistor-controlled heating pad\n5. Monitor temperature using rectal thermometer", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/2618/clip_2_removed_step_3.mp4"}} |
| {"qid": "ExpVid_4809", "question": "What is the correct sequence of steps for the Intracellular Staining and Final Preparation experiment for flow cytometry analysis of cytokine production?", "options": ["A. 1. Wash cells in 1 ml wash buffer\n2. Resuspend pellets\n3. Resuspend in 100 μl cold fixation solution\n4. Incubate for 10 minutes at 4°C in dark\n5. Mix by vortexing\n6. Wash cells in 1 ml wash buffer\n7. Resuspend pellets in 90 μl permeabilization buffer\n8. Stain with 10 μl intracellular antibodies and mix by vortexing\n9. Incubate for 30 minutes in dark at 4°C\n10. Wash cells twice in 1 ml fresh permeabilization buffer\n11. Resuspend samples in 400 μl fresh permeabilization buffer\n12. Mix by vortexing and place cells on ice for flow cytometry analysis", "B. 1. Wash cells in 1 ml wash buffer\n2. Resuspend pellets\n3. Resuspend in 100 μl cold fixation solution\n4. Mix by vortexing\n5. Incubate for 10 minutes at 4°C in dark\n6. Resuspend pellets in 90 μl permeabilization buffer\n7. Wash cells in 1 ml wash buffer\n8. Stain with 10 μl intracellular antibodies and mix by vortexing\n9. Incubate for 30 minutes in dark at 4°C\n10. Wash cells twice in 1 ml fresh permeabilization buffer\n11. Resuspend samples in 400 μl fresh permeabilization buffer\n12. Mix by vortexing and place cells on ice for flow cytometry analysis", "C. 1. Wash cells in 1 ml wash buffer\n2. Resuspend pellets\n3. Resuspend in 100 μl cold fixation solution\n4. Mix by vortexing\n5. Incubate for 10 minutes at 4°C in dark\n6. Wash cells in 1 ml wash buffer\n7. Stain with 10 μl intracellular antibodies and mix by vortexing\n8. Incubate for 30 minutes in dark at 4°C\n9. Resuspend pellets in 90 μl permeabilization buffer\n10. Wash cells twice in 1 ml fresh permeabilization buffer\n11. Resuspend samples in 400 μl fresh permeabilization buffer\n12. Mix by vortexing and place cells on ice for flow cytometry analysis", "D. 1. Wash cells in 1 ml wash buffer\n2. Resuspend pellets\n3. Resuspend in 100 μl cold fixation solution\n4. Mix by vortexing\n5. Incubate for 10 minutes at 4°C in dark\n6. Wash cells in 1 ml wash buffer\n7. Resuspend pellets in 90 μl permeabilization buffer\n8. Stain with 10 μl intracellular antibodies and mix by vortexing\n9. Incubate for 30 minutes in dark at 4°C\n10. Wash cells twice in 1 ml fresh permeabilization buffer\n11. Resuspend samples in 400 μl fresh permeabilization buffer\n12. Mix by vortexing and place cells on ice for flow cytometry analysis"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/54615/clip_7.mp4"}} |
| {"qid": "ExpVid_6606", "question": "Given the following step list，which step was not performed in the video?\n1. Prepare 25 microliters of PCR mix on ice\n2. Add one microliter of DNA template to PCR mix\n3. Set PCR cycling conditions for gene amplification\n4. Mix five microliters of PCR sample with one microliter of 5X DNA loading dye\n5. Run sample on 1% agarose gel to verify amplification", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/63101/clip_8_removed_step_1.mp4"}} |
| {"qid": "ExpVid_7575", "question": "The study concludes that a _____ protocol rapidly enriches _____ from human postmortem brain, validated by phosphorylated tau partitioning into the _____ fraction while native EEA1 remains in the _____ fraction.", "options": null, "answer": "single-step sarkosyl-based ultracentrifugation | detergent-insoluble protein aggregates | P2 | S1", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/55835_.mp4"}} |
| {"qid": "ExpVid_6567", "question": "Given the following step list，which step was not performed in the video?\n1. Place demineralized bones in 50 ml centrifuge tube with 1:1 methanol-chloroform mixture\n2. Wrap tube with tin foil to prevent light exposure and chloroform decomposition\n3. Place tube on orbital shaker for 1 hour\n4. Transfer bones with tweezers to new tube containing methanol wrapped with tin foil\n5. Remove methanol completely from tube\n6. Wash bones with distilled water twice for 15 minutes each on orbital shaker\n7. Decant final wash water and proceed under sterile conditions", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/59271/clip_3_removed_step_3.mp4"}} |
| {"qid": "ExpVid_6381", "question": "Given the following step list，which step was not performed in the video?\n1. Apply rubber cement to affix 2-3 pieces of Velcro to each of two 6x12 inch gel packs\n2. Ensure Velcro pieces are equally spaced on gel packs\n3. Allow 24 hours for rubber cement to dry\n4. Fold 12x16 inch thick fabric in half lengthwise\n5. Place gel packs lengthwise along each inner half of folded fabric", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/50849/clip_2_removed_step_3.mp4"}} |
| {"qid": "ExpVid_7133", "question": "In PDA-assisted reduction on nanodiamonds, increasing the diamine silver hydroxide concentration from ____ to ____ led to silver nanoparticles whose TEM-measured diameters increased from approximately ____ to ____, with a higher surface density observed.", "options": null, "answer": "0.4 mg/mL | 0.6 mg/mL | 24 nm | 28 nm", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/58458_.mp4"}} |
| {"qid": "ExpVid_5190", "question": "What is the correct sequence of steps for completing the bilayer construct in the 'PEG fibrin addition and final assembly' experimental procedure?", "options": ["A. 1. Prepare PEG fibrin gel using 250 microliters of cell culture medium\n2. Layer pegylated fibrinogen thrombin solution over ASC-CSM-collagen layers\n3. Place 1 milliliter of medium in upper chamber over construct\n4. Incubate constructs for 30 minutes in 5% CO₂ humidified incubator to achieve gelation\n5. Place 3 milliliters of medium in lower chamber", "B. 1. Layer pegylated fibrinogen thrombin solution over ASC-CSM-collagen layers\n2. Prepare PEG fibrin gel using 250 microliters of cell culture medium\n3. Incubate constructs for 30 minutes in 5% CO₂ humidified incubator to achieve gelation\n4. Place 1 milliliter of medium in upper chamber over construct\n5. Place 3 milliliters of medium in lower chamber", "C. 1. Prepare PEG fibrin gel using 250 microliters of cell culture medium\n2. Layer pegylated fibrinogen thrombin solution over ASC-CSM-collagen layers\n3. Place 1 milliliter of medium in upper chamber over construct\n4. Place 3 milliliters of medium in lower chamber\n5. Incubate constructs for 30 minutes in 5% CO₂ humidified incubator to achieve gelation", "D. 1. Prepare PEG fibrin gel using 250 microliters of cell culture medium\n2. Layer pegylated fibrinogen thrombin solution over ASC-CSM-collagen layers\n3. Incubate constructs for 30 minutes in 5% CO₂ humidified incubator to achieve gelation\n4. Place 1 milliliter of medium in upper chamber over construct\n5. Place 3 milliliters of medium in lower chamber"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/3953/clip_5.mp4"}} |
| {"qid": "ExpVid_7509", "question": "The study’s key advance was a _____ three-dimensional culture that preserved native _____-rich architecture and drove osteosarcoma cells to exhibit _____ and _____, creating an in vitro platform to probe bone tumor development, progression, and drug sensitivities.", "options": null, "answer": "decellularized bone extracellular matrix (BEM) | Collagen I/IV | phenotypic heterogeneity | dedifferentiation", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/59271_.mp4"}} |
| {"qid": "ExpVid_7659", "question": "By decellularizing human ______ ECM to create a hydrogel that retains basal lamina components—Collagen-IV ______ and the glomerular ______ isoforms, plus laminins—the researchers produced a material that modulated HKMEC ______ and supports kidney microphysiological studies.", "options": null, "answer": "kidney cortex | A1/A2 | A3/A5 | CD31 distribution", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/58314_.mp4"}} |
| {"qid": "ExpVid_7727", "question": "According to the demonstration, the ____ system enables high-recovery, reproducible liquid-phase molecular-weight–based fractionation of intact proteins via ____ with automated time-based collection, yielding discrete fractions spanning ____ that directly support enhanced ____ analyses.", "options": null, "answer": "GELFREE 8100 | electrophoretic elution from stacking/resolving gels | 3.5–150 kDa | top-down and bottom-up proteomics", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/1842_.mp4"}} |
| {"qid": "ExpVid_5271", "question": "What is the correct sequence of steps for the experimental procedure titled 'Surface treatment and cell seeding'?", "options": ["A. 1. Treat MEA surface with 10 micrograms per milliliter fibronectin for 1-2 hours\n2. Wash MEA with DI water\n3. Air dry MEA in laminar flow hood\n4. Harvest adherent mammalian cells using trypsin ETTA\n5. Resuspend cells to concentration of 100-200 cells per microliter in 1 milliliter cell media\n6. Place 20-30 microliter drop of cell-containing media on MEA\n7. Transfer Petri dish with MEA to humidified incubator at 37°C and 5% CO₂", "B. 1. Harvest adherent mammalian cells using trypsin ETTA\n2. Resuspend cells to concentration of 100-200 cells per microliter in 1 milliliter cell media\n3. Treat MEA surface with 10 micrograms per milliliter fibronectin for 1-2 hours\n4. Wash MEA with DI water\n5. Air dry MEA in laminar flow hood\n6. Place 20-30 microliter drop of cell-containing media on MEA\n7. Transfer Petri dish with MEA to humidified incubator at 37°C and 5% CO₂", "C. 1. Treat MEA surface with 10 micrograms per milliliter fibronectin for 1-2 hours\n2. Harvest adherent mammalian cells using trypsin ETTA\n3. Wash MEA with DI water\n4. Air dry MEA in laminar flow hood\n5. Resuspend cells to concentration of 100-200 cells per microliter in 1 milliliter cell media\n6. Place 20-30 microliter drop of cell-containing media on MEA\n7. Transfer Petri dish with MEA to humidified incubator at 37°C and 5% CO₂", "D. 1. Treat MEA surface with 10 micrograms per milliliter fibronectin for 1-2 hours\n2. Wash MEA with DI water\n3. Harvest adherent mammalian cells using trypsin ETTA\n4. Resuspend cells to concentration of 100-200 cells per microliter in 1 milliliter cell media\n5. Air dry MEA in laminar flow hood\n6. Place 20-30 microliter drop of cell-containing media on MEA\n7. Transfer Petri dish with MEA to humidified incubator at 37°C and 5% CO₂"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/4415/clip_2.mp4"}} |
| {"qid": "ExpVid_5342", "question": "What is the correct sequence of steps for composite sample fabrication in the micro-annular gas flow experiment?", "options": ["A. 1. Construct sample from two grade B electric resistance welded steel pipes\n2. Drill four evenly spaced 3.2 mm MPT threaded holes at pipe top/bottom for fittings\n3. Drill holes in outer pipe for pressure release and rock porosity mimicry\n4. Fabricate custom steel coupling for pipe connection\n5. Thread inner pipe to connect with coupling\n6. Check pipe-coupling fit and remove pipe\n7. Weld anti-corrosion treated outer pipe to steel plate ring\n8. Screw lubricated inner pipe into coupling to complete assembly", "B. 1. Construct sample from two grade B electric resistance welded steel pipes\n2. Drill holes in outer pipe for pressure release and rock porosity mimicry\n3. Drill four evenly spaced 3.2 mm MPT threaded holes at pipe top/bottom for fittings\n4. Fabricate custom steel coupling for pipe connection\n5. Thread inner pipe to connect with coupling\n6. Check pipe-coupling fit and remove pipe\n7. Weld anti-corrosion treated outer pipe to steel plate ring\n8. Screw lubricated inner pipe into coupling to complete assembly", "C. 1. Construct sample from two grade B electric resistance welded steel pipes\n2. Drill holes in outer pipe for pressure release and rock porosity mimicry\n3. Drill four evenly spaced 3.2 mm MPT threaded holes at pipe top/bottom for fittings\n4. Weld anti-corrosion treated outer pipe to steel plate ring\n5. Fabricate custom steel coupling for pipe connection\n6. Thread inner pipe to connect with coupling\n7. Check pipe-coupling fit and remove pipe\n8. Screw lubricated inner pipe into coupling to complete assembly", "D. 1. Construct sample from two grade B electric resistance welded steel pipes\n2. Fabricate custom steel coupling for pipe connection\n3. Drill holes in outer pipe for pressure release and rock porosity mimicry\n4. Drill four evenly spaced 3.2 mm MPT threaded holes at pipe top/bottom for fittings\n5. Thread inner pipe to connect with coupling\n6. Check pipe-coupling fit and remove pipe\n7. Weld anti-corrosion treated outer pipe to steel plate ring\n8. Screw lubricated inner pipe into coupling to complete assembly"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/52098/clip_1.mp4"}} |
| {"qid": "ExpVid_4691", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Weigh and mince hind limb skeletal muscle from 20-week-old C57BL/6 male mice in ice-cold PBS\n2. Place minced skeletal muscle tissue in 50 ml conical tube containing 10 ml PBS on ice\n3. Centrifuge sample at 300 × g at 4°C for 5 minutes\n4. Remove PBS supernatant by aspiration\n5. Estimate pellet volume using reference tubes with 1-4 ml water\n6. Add 7 volumes ice-cold 1% formaldehyde in PBS to pellet\n7. Homogenize samples on ice using probe tissue homogenizer\n8. Cross-link sample by incubating at room temperature for 10 minutes\n9. Quench cross-linking reaction by adding 1M glycine to 0.125M final concentration\n10. Incubate quenched sample for 5 minutes at room temperature\n11. Centrifuge sample at 3,000 × g for 5 minutes at 4°C\n12. Remove supernatant via aspiration\n13. Rinse pellet by resuspending in 10 ml ice-cold base buffer with freshly added protease inhibitors\n14. Centrifuge the sample\n15. Remove supernatant by aspiration\n16. Resuspend pellet in 6 ml lysis buffer with freshly added protease inhibitors\n17. Transfer sample to pre-chilled 15 ml Dounce homogenizer\n18. Incubate sample for 10 minutes on ice\n19. Homogenize sample on ice with 15 slow strokes using loose pestle\n20. Homogenize sample with 15 strokes using tight pestle to release nuclei\n21. Filter homogenate through cell strainer\n22. Rinse with 4 ml lysis buffer\n23. Centrifuge samples at 1,000 × g for 10 minutes at 4°C\n24. Resuspend pellet in 5 ml ice-cold base buffer\n25. Dounce homogenize 10 times with tight pestle to release nuclei\n26. Filter suspension through cell strainer\n27. Rinse tube with 2 ml base buffer\n28. Filter suspension again\n29. Repeat filtration with gradually reduced pore size cell strainers\n30. Centrifuge at 1,000 × g at 4°C for 10 minutes\n31. Remove supernatant\n32. Resuspend pellet in 500 μl base buffer\n33. Centrifuge again (implied same parameters)\n34. Discard supernatant\n35. Store pellet at -80°C\n36\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "14, 15, 16, 17, 18, 19, 20", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/56013/clip_3.mp4"}} |
| {"qid": "ExpVid_4055", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Measure 150 milliliters of acetone using graduated cylinder\n2. Transfer measured acetone to 250 milliliter Erlenmeyer flask\n3. Add 30 grams of commercial gliadin powder to flask while stirring\n4. Seal flask opening with aluminum foil\n5. Stir solution continuously at room temperature overnight\n6. Filter solution through filter paper\n7. Wash retentate with approximately 50 milliliters of fresh acetone\n8. Transfer retentate and filter paper to square cell culture dish\n9. Cover dish with large filter paper to slow acetone evaporation\n10. Transfer dried defatted gliadin to airtight container\n11. Store container on lab bench\n12. Measure 150 milliliters of deionized water using a graduated cylinder\n13. Transfer measured deionized water to a 1,000 milliliter Erlenmeyer flask\n14. Measure 350 milliliters of absolute ethanol using a mess cylinder\n15. Add measured absolute ethanol to the Erlenmeyer flask containing deionized water\n16. Stir solution vigorously until air bubbles are no longer observed\n17. Add 20 grams of defatted gliadin powder to the stirring solution\n18. Transfer entire solution to a 1,000 milliliter mess cylinder\n19. Allow solution to sit for two days\n20. Transfer supernatant to round-bottomed flask using flexible tubing\n21. Remove ethanol from supernatant via rotary evaporation\n22. Freeze solution containing gliadin aggregates by immersing flask in methanol dry ice mixture\n23. Freeze-dry solution at -70 degrees Celsius under vacuum\n24. Crush freeze-dried gliadin with mortar and pestle\n25. Grind crushed gliadin with coffee grinder to obtain fine powder\n26. Transfer powder into airtight container\n27. Store powder at room temperature\n28. Add 3.2 grams of distilled water and 6.8 grams of absolute ethanol to a tared scintillation vial\n29. Stir solution vigorously until air bubbles are no longer observed\n30. Add 40 microliters of four normal hydrochloric acid to vial while st\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "20, 21, 22, 23, 24, 25, 26, 27", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/54147/clip_4.mp4"}} |
| {"qid": "ExpVid_6447", "question": "Given the following step list，which step was not performed in the video?\n1. Select 'Load Array List' in software interface\n2. Upload GAL array mapping file", "options": ["A. 1", "B. 2"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/56094/clip_15_removed_step_1.mp4"}} |
| {"qid": "ExpVid_6374", "question": "Given the following step list，which step was not performed in the video?\n1. Pin arms and legs of euthanized mouse to dissection board\n2. Make midline incision on ventral side with scissors\n3. Gently pull skin to expose peritoneum\n4. Make incisions on peritoneum to expose abdominal organs\n5. Hold tip of sternum with forceps\n6. Puncture diaphragm with scissors\n7. Cut diaphragm along rib cage sides with scissors parallel to dissection board\n8. Cut through thoracic cavity along dorsoventral line", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7", "H. 8"], "answer": "G", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/2702/clip_4_removed_step_7.mp4"}} |
| {"qid": "ExpVid_4558", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Boil 0.4-0.6 mm thick viscose non-woven fibrous material\n2. Dry boiled viscose material\n3. Iron dried viscose material\n4. Cut material into 11 mm circles using metal hole punch\n5. Place cut fibrous material between two slides\n6. Wrap slides in foil\n7. Autoclave foil-wrapped slides\n8. Place inserts into wells of sterile 12-well plate\n9. Place each sterilized scaffold into corresponding insert\n10. Mix 2.7 × 10⁵ fibroblasts in 100 microliters fetal calf serum with 100 microliters freshly diluted thrombin per scaffold\n11. Dispense thrombin-fibroblast mixture onto each scaffold\n12. Add 200 microliters freshly diluted fibrinogen to each scaffold\n13. Mix cell mixture within each well using gentle pipetting to distribute solution evenly over scaffold\n14. Prepare test gel in control well without scaffold to track clot formation time\n15. Assess clot formation using pipette tip or by inverting plate\n16. Submerge organ cultures in medium supplemented with ascorbic acid and TGF-beta 1\n17. Replace medium with RFAD medium containing 500 units/mL aprotinin\n18. Seed 2.5 × 10⁵ mTEC suspended in 100 μL RFAD medium onto each scaffold\n19. Incubate co-cultures for 24 hours\n20. Replace medium with fresh medium containing 250 units per milliliter of aprotinin\n21. Use forceps to separate scaffold dermal equivalent from filter\n22. Embed OTCs in OCT compound\n23. Freeze OTCs in gaseous phase over liquid nitrogen\n24. Separate scaffold tissue from filters\n25. Cut OTCs into pieces using scalpel\n26. Transfer OTC pieces into 2 ml RNA-free tube containing 1 ml denaturing solution\n27. Mechanically shred OTCs using fast prep instrument at 6.0 speed for 30 seconds\n28. Perform second shredding cycle at 6.0 speed for 30 seconds after 2-minute rest on ice\n29. Isolate RNA using acid guanidinium thiocyanate-phenol-chloroform extraction per manufacturer protocol\n30. Remove membrane and separate scaffold from fibrin fib\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "10, 11, 12, 13, 14, 15", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/52614/clip_2.mp4"}} |
| {"qid": "ExpVid_6933", "question": "Given the following step list，which step was not performed in the video?\n1. Treat cells with 0.5 mM and 0.15 mM glyoxal solution for 1 hour\n2. Perform immunoblotting with anti-AGE antibodies to detect glycation\n3. Count bacterial colonies and normalize counts to untreated control", "options": ["A. 1", "B. 2", "C. 3"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/60692/clip_8_removed_step_1.mp4"}} |
| {"qid": "ExpVid_6070", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Seed HEK293T cells in poly-L-lysine coated culture dish\n2. Culture seeded HEK293T cells at 37°C in tissue culture incubator for one day\n3. Transfect HEK293T cells with mt-Keima lentiviral DNA and packaging DNase using transfection reagent according to manufacturer's instructions\n4. Remove transfection media\n5. Add 8 milliliters of fresh media\n6. Collect media containing viral particles\n7. Remove cellular debris by centrifugation\n8. Filter virus-containing media using 0.45 micrometer syringe filter\n9. Plate HeLa-Parkin cells\n10. Plate HeLa-Parkin cells in 60 millimeter culture dish\n11. Culture cells with 4 milliliters of growth media at 37°C in incubator for one day\n12. Remove growth medium\n13. Add 1 milliliter of mt-Keima virus media\n14. Add 2 milliliters of growth medium containing 3 microliters polybrene stock solution\n15. Incubate cells for one day in CO₂ incubator\n16. Remove virus-containing media\n17. Wash cells twice with PBS\n18. Add four milliliters of growth media\n19. Treat cells with two micrograms per microliter puromycin for two days\n20. Prepare fresh growth media containing 10 micromolar CCCP\n21. Remove cultured media from cells\n22. Add 4 milliliters of fresh CCCP-containing growth media to cells\n23. Incubate cells at 37°C in CO₂ incubator for 6 hours\n24. Remove growth medium\n25. Wash cells once with PBS\n26. Detach cells\n27. Treat cells with trypsin EDTA solution\n28. Collect cells by centrifugation\n29. Re-suspend cells in 1 milliliter of PBS\n30. Transfer cells to FACS tube\n31. Place FACS tube on ice\n32. Turn on flow cytometer\n33. Start flow cytometry analysis software\n34. Generate new experiment in software\n35. Configure parameter window: select BV605 for violet laser and PE-CF594 for yellow-green laser\n36. Run control sample of uninfected mt-Keima lentivirus cells\n37. Draw polygon gate around \nAnswer with the step number only.", "options": null, "answer": "37", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/58099/clip_7_prediction.mp4"}} |
| {"qid": "ExpVid_7722", "question": "The study demonstrates a methodological innovation in which a freely floating ____ is formed by a ____ that co-confines ____ and ____ waves, providing a platform for multi-wave detection.", "options": null, "answer": "water fiber | high-voltage water bridge | capillary | electromagnetic", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/58174_.mp4"}} |
| {"qid": "ExpVid_7294", "question": "According to the results, compared with rough aggregate surfaces, the ______ around ______ exhibited ______ at ______ from the aggregate.", "options": null, "answer": "ITZ | smooth aggregate surfaces | lower porosity | nearly every distance", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/60245_.mp4"}} |
| {"qid": "ExpVid_4761", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Weigh 2 grams of PEG in a 40 milliliter glass vial\n2. Add approximately 308 microliters of methacrylic anhydride to the vial\n3. Loosely close vial with hard plastic cap\n4. Microwave vial on high for 2 minutes in standard domestic microwave\n5. Vortex vial thoroughly while wearing heat-resistant gloves\n6. Microwave solution on high for additional 5 minutes\n7. Allow vial to cool briefly until handleable\n8. Uncap vial while swirling\n9. Slowly mix in methylene chloride while vortexing until solution is clear and homogeneous\n10. Add solution dropwise to 200 milliliters of stirred cold diethyl ether\n11. Collect precipitated PEG DM by vacuum filtration\n12. Allow collected PEG DM to dry\n13. Dissolve dried PEG DM in 100-150 milliliters of deionized water\n14. Dialyze solution against deionized water for 5 days using dialysis tubing with 1000 Dalton MWCO\n15. Transfer dialyzed solution into containers\n16. Freeze solution at -80°C overnight\n17. Lyophilize solution for 4 days to obtain purified PEG DM powder\n18. Aspirate medium from culture flask\n19. Rinse cells with 37°C calcium/magnesium-free HBSS\n20. Aspirate HBSS from culture flask\n21. Add 2 ml of 37°C trypsin/EDTA solution to flask\n22. Incubate cells at 37°C for 3 minutes\n23. Firmly jar side of flask to detach cells\n24. Add 8 ml of 37°C medium to flask\n25. Vigorously pipette cell suspension up/down without introducing bubbles\n26. Transfer cell solution to sterile 15 ml centrifuge tube\n27. Count cells using hemocytometer to determine total cells for infection\n28. Add cell suspension to achieve 400,000 cells per well in six-well suspension culture plate\n29. Aliquot 50 microliters of HBSS per well into 1.8 milliliter microcentrifuge tube\n30. Pipette virus suspension into prepared HBSS\n31. Add diluted virus to each well to achieve target MOI\n32. Add medium to each well to achieve 1.7 milliliters total volume\n33. Place plate on orbital shake\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "28, 29, 30, 31, 32, 33, 34, 35", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/3521/clip_4.mp4"}} |
| {"qid": "ExpVid_4434", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Make Y incision to peritoneal cavity using dissecting scissors and forceps\n2. Excise uterus containing E9.5 embryo\n3. Dissect embryos in complete DMEM growth medium\n4. Remove yolk sac, placenta, and surrounding membranes using forceps\n5. Rinse isolated embryos in HBSS to remove residual amniotic tissue and blood\n6. Prepare fixative by adding paraformaldehyde to HBSS\n7. Adjust solution to 4% paraformaldehyde working concentration\n8. Add 1 milliliter of fixative solution to each well of 24-well plate\n9. Place embryos into individual wells\n10. Incubate embryos for 45 minutes with gentle agitation on rocker\n11. Remove fixative after incubation\n12. Remove fixative from embryos\n13. Wash embryos thrice for 30 minutes in PBS with calcium, magnesium, and 0.1% Triton\n14. Incubate embryos in blocking solution with gentle agitation for one hour\n15. Incubate embryos in blocking solution with gentle agitation for one hour (second incubation)\n16. Rinse embryos using fresh blocking solution\n17. Prepare primary antibody solution by diluting antibodies in supplemented PBS\n18. Remove blocking solution from wells\n19. Add 1 mL of primary antibody solution to each well\n20. Incubate plate at 4°C with gentle rotation for three days\n21. Wash embryos with supplemented PBS five times for one hour on rocker at 20 RPM\n22. Add 1 milliliter of secondary antibody solution to each well\n23. Incubate plate with gentle rocking at 4°C in the dark for three days\n24. Remove secondary antibody solution\n25. Wash embryos three times for 30 minutes each in supplemented PBS\n26. Prepare 4% (w/v) low melting point agarose solution in PBS with calcium and magnesium\n27. Place 12-well plate in 55°C bead bath\n28. Add 3 ml of agarose solution to each well\n29. Transfer plate to bench top\n30. Transfer embryos into individual wells using perforated spoon\n31. Use pipette tips to gently embed and orient embryo centered in solution\n\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "38, 39, 40, 41, 42, 43", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/64100/clip_8.mp4"}} |
| {"qid": "ExpVid_4180", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Take suspension of HepG2/C3A and THLE-3 cells\n2. Count number of cells and dilute suspension in complete growth media to obtain 1 million cells in maximum 1.5 milliliters volume\n3. Wash wells of ultra-low attachment 24-well round bottom plate with 0.5 milliliters of growth media\n4. Centrifuge plate at 3,000g for five minutes\n5. Transfer cell suspension to plate\n6. Centrifuge plate at 120g for three minutes\n7. Incubate plate to initiate spheroid formation\n8. Fill humidity chamber with 25 milliliters of sterile water using 10-milliliter syringe with long needle\n9. Fill cell chamber with 9 milliliters of growth media using 10-milliliter syringe with long needle\n10. Place bioreactor in 3D incubator at 37°C with 5% CO₂ for 24 hours\n11. Pipette spheroids up and down gently using 1-mL wide-bore tips to detach them from ultra-low attachment plate\n12. Transfer detached spheroids to tissue culture dish\n13. Wash plate with 0.5 mL prewarmed growth media to capture remaining spheroids\n14. Transfer washed spheroids to culture dish\n15. Evaluate spheroid size, compactness, and roundness using light microscope\n16. Select adequately formed spheroids\n17. Transfer selected spheroid to equilibrating bioreactor containing 5 mL fresh growth media\n18. Fill entire bioreactor with fresh growth media\n19. Position bioreactor in 3D incubator\n20. Adjust rotation speed using control unit\n21. Replace 10 milliliters of old growth media with 10 milliliters of fresh media\n22. Culture spheroids for 15 days\n23. Divide cultured spheroids between two fresh bioreactors\n24. Open 3D app installed on tablet\n25. Select bioreactor in app\n26. Capture image of bioreactor\n27. Place black background behind bioreactor (alternative method)\n28. Ensure no light reflects on cell chamber\n29. Capture image close to bioreactor\n30. Remove 5 mL media from bioreactor using syringe with long needle via top port\n31. Ensure spheroids desc\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/65086/clip_5.mp4"}} |
| {"qid": "ExpVid_7420", "question": "The study showed that a single intraperitoneal dose of ____ at ____ selectively injures ____ in mice, producing ____ and enabling a fast, reproducible model of pure small fiber neuropathy.", "options": null, "answer": "resiniferatoxin (RTX) | 50 μg/kg | peripherin-positive small-diameter neurons | IENF degeneration and ATF-3 induction", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/56651_.mp4"}} |
| {"qid": "ExpVid_7184", "question": "According to the viability analysis, spheroid cell death, inferred from the release of _____, rose to about _____ by _____ and then decreased to _____ thereafter.", "options": null, "answer": "adenylate kinase | 7% | day 17 | below 5%", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/65086_.mp4"}} |
| {"qid": "ExpVid_4241", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Generate extrachromosomal array with translational construct of target genes\n2. Obtain transgenic lines from Caenorhabditis Genetic Center or published research laboratory\n3. Expose worms to gamma radiation to integrate extrachromosomal lines into genome\n4. Select stably expressed lines showing marker protein expression (e.g., GFP or roller)\n5. Outcross lines at least two times to remove radiation-induced mutations\n6. Streak OP50 clone on agar plate\n7. Culture streaked bacteria in liquid LB broth overnight\n8. Seed NGM plates with overnight liquid OP50 culture\n9. Incubate seeded plates at 37°C overnight to grow bacterial lawn\n10. Cool down incubated plates at room temperature for at least 30 minutes\n11. Grow transgenic worm line at 20°C without starvation for ≥2 generations\n12. Pick 8-10 young gravid adults to new worm plate\n13. Allow adults to lay eggs for 3-5 hours\n14. Remove all adults from plates\n15. Synchronize worms by letting eggs hatch and grow larvae for ≈48 hours to L4 stage\n16. Heat-stress worms by incubating plates with L4 larvae at 35°C for 2-5 hours\n17. Place thermometer in incubator to monitor temperature\n18. Pipette 10 microliters of M9 buffer onto center of glass slide\n19. Pick heat-shocked worms\n20. Transfer worms into M9 buffer drop on slide\n21. Wash worms off plate using M9 buffer (alternative method)\n22. Centrifuge sample at 1000 × g at room temperature for 1 minute\n23. Discard supernatant\n24. Add worms to glass slide\n25. Add worms to glass slide\n26. Drain excess liquid using soft tissue under dissecting microscope\n27. Add 10 microliters of 95% alcohol onto worms under microscope while observing\n28. Repeat ethanol addition twice after previous ethanol dries\n29. Add 10 microliters of DAPI to worms\n30. Add 10 microliters of DAPI to the worms\n31. Apply cover slip to slide\n32. Seal slide with transparent nail polish gel\n33. Allow nail polish to set for 10 minut\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "18, 19, 20, 21, 22, 23, 24", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57914/clip_4.mp4"}} |
| {"qid": "ExpVid_7295", "question": "_____ showed that adding _____ or _____ as additives led to full conversion of _____ into perovskite, with no residual peak.", "options": null, "answer": "X-ray diffraction | NaI | CuBr | PbI2", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/55307_.mp4"}} |
| {"qid": "ExpVid_6735", "question": "Given the following step list，which step was not performed in the video?\n1. Incise aorta proximal to SMA using scissors\n2. Incise aorta distal to SMA to release cannula\n3. Place 5-0 silk suture knot on proximal ileocecal junction\n4. Place 5-0 silk suture knot on distal sigmoid colon\n5. Cut terminal ileum and colon between suture knots\n6. Remove large intestine\n7. Cut small intestine at duodenojejunal junction", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/50658/clip_4_removed_step_1.mp4"}} |
| {"qid": "ExpVid_6693", "question": "Given the following step list，which step was not performed in the video?\n1. Wash cells with PBST three times for 10 minutes each using WellMate and multi-channel aspirator\n2. Add 15 microliters of fluorescently labeled secondary antibody with nuclear stain to each well using multi-channel pipetter\n3. Cover plate with foil to protect from light\n4. Incubate plates at room temperature for one hour\n5. Wash cells with PBST three times for 10 minutes each as previously performed\n6. Add 30 microliters of PBST to cells\n7. Seal plate with clear sealing film\n8. Cover sealed plate with foil\n9. Store sealed plates at 4°C for up to three weeks before imaging", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7", "H. 8", "I. 9"], "answer": "G", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/2137/clip_9_removed_step_7.mp4"}} |
| {"qid": "ExpVid_7592", "question": "The study’s key advance was a culture system that enables real-time monitoring of bacterial growth with minimal volumes by cultivating cells at the ______ level inside ______ prepared by the ______, then immobilizing them on a supported bilayer via ______.", "options": null, "answer": "single-cell | giant vesicles | droplet transfer method | biotin-PEG-DSPE/neutravidin", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/59555_.mp4"}} |
| {"qid": "ExpVid_6326", "question": "Given the following step list，which step was not performed in the video?\n1. Return the nerve to its original position\n2. Cover the nerve with overlying muscles\n3. Treat the mice with proper analgesia\n4. Close the skin with 5-0 nylon sutures\n5. Place the mouse alone in a clean cage for observation\n6. Provide heat support during recovery until the mouse regains sufficient consciousness to maintain sternal recumbency", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6"], "answer": "E", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/56857/clip_6_removed_step_5.mp4"}} |
| {"qid": "ExpVid_5188", "question": "What is the correct sequence of steps for the plate assembly and membrane coating procedure?", "options": ["A. 1. Unpack plate and inserts using sterilized forceps\n2. Coat porous membrane of each insert with 90 microliters of 10 μg/μL collagen IV/fibronectin mixture\n3. Assemble 12-well plate in biological safety cabinet\n4. Grab inserts at base with forceps and place into wells\n5. Incubate plate at 37°C for 24 hours in cell culture incubator", "B. 1. Assemble 12-well plate in biological safety cabinet\n2. Unpack plate and inserts using sterilized forceps\n3. Grab inserts at base with forceps and place into wells\n4. Coat porous membrane of each insert with 90 microliters of 10 μg/μL collagen IV/fibronectin mixture\n5. Incubate plate at 37°C for 24 hours in cell culture incubator", "C. 1. Assemble 12-well plate in biological safety cabinet\n2. Unpack plate and inserts using sterilized forceps\n3. Coat porous membrane of each insert with 90 microliters of 10 μg/μL collagen IV/fibronectin mixture\n4. Grab inserts at base with forceps and place into wells\n5. Incubate plate at 37°C for 24 hours in cell culture incubator", "D. 1. Unpack plate and inserts using sterilized forceps\n2. Assemble 12-well plate in biological safety cabinet\n3. Grab inserts at base with forceps and place into wells\n4. Coat porous membrane of each insert with 90 microliters of 10 μg/μL collagen IV/fibronectin mixture\n5. Incubate plate at 37°C for 24 hours in cell culture incubator"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/60692/clip_1.mp4"}} |
| {"qid": "ExpVid_5446", "question": "What is the correct sequence of steps for the cell culture and harvesting experimental procedure?", "options": ["A. 1. Observe unstained Aa23 and Aa23-T cells at 100x magnification using light microscopy\n2. Culture cells for five to seven days to reach 70 to 80% confluence per flask\n3. Culture cells for five to seven days to reach 90 to 100% confluence\n4. Transfer 1 mL of culture medium to microcentrifuge tube using pipette\n5. Centrifuge at 100 RCF for five minutes to harvest cells\n6. Remove supernatant and store cell pellet at -20°C", "B. 1. Culture cells for five to seven days to reach 70 to 80% confluence per flask\n2. Observe unstained Aa23 and Aa23-T cells at 100x magnification using light microscopy\n3. Transfer 1 mL of culture medium to microcentrifuge tube using pipette\n4. Centrifuge at 100 RCF for five minutes to harvest cells\n5. Remove supernatant and store cell pellet at -20°C\n6. Culture cells for five to seven days to reach 90 to 100% confluence", "C. 1. Observe unstained Aa23 and Aa23-T cells at 100x magnification using light microscopy\n2. Culture cells for five to seven days to reach 70 to 80% confluence per flask\n3. Transfer 1 mL of culture medium to microcentrifuge tube using pipette\n4. Centrifuge at 100 RCF for five minutes to harvest cells\n5. Remove supernatant and store cell pellet at -20°C\n6. Culture cells for five to seven days to reach 90 to 100% confluence", "D. 1. Culture cells for five to seven days to reach 70 to 80% confluence per flask\n2. Transfer 1 mL of culture medium to microcentrifuge tube using pipette\n3. Observe unstained Aa23 and Aa23-T cells at 100x magnification using light microscopy\n4. Centrifuge at 100 RCF for five minutes to harvest cells\n5. Remove supernatant and store cell pellet at -20°C\n6. Culture cells for five to seven days to reach 90 to 100% confluence"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/63662/clip_2.mp4"}} |
| {"qid": "ExpVid_6908", "question": "Given the following step list，which step was not performed in the video?\n1. Air-dry RNA pellet for 5 minutes\n2. Add 50 microliters of nuclease-free water to RNA pellet\n3. Resuspend RNA pellet in nuclease-free water\n4. Treat samples with 1 microliter DNase to remove DNA\n5. Inactivate DNase and cations\n6. Store RNA samples in 2.0-milliliter microcentrifuge tubes at -80°C", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/56423/clip_7_removed_step_3.mp4"}} |
| {"qid": "ExpVid_5414", "question": "What is the correct sequence of steps for the Photoactivation and time-lapse acquisition experimental procedure?", "options": ["A. 1. Establish micron-scale photoactivation regions beneath individual cells using software control\n2. Start time-lapse acquisition with 80 time points at 5-second intervals\n3. Select new field of cells and repeat process after time-lapse completion\n4. Photoactivate selected regions by opening digital diaphragm shutter for 1-1.5 seconds after acquiring 10 time points", "B. 1. Establish micron-scale photoactivation regions beneath individual cells using software control\n2. Start time-lapse acquisition with 80 time points at 5-second intervals\n3. Photoactivate selected regions by opening digital diaphragm shutter for 1-1.5 seconds after acquiring 10 time points\n4. Select new field of cells and repeat process after time-lapse completion", "C. 1. Start time-lapse acquisition with 80 time points at 5-second intervals\n2. Establish micron-scale photoactivation regions beneath individual cells using software control\n3. Photoactivate selected regions by opening digital diaphragm shutter for 1-1.5 seconds after acquiring 10 time points\n4. Select new field of cells and repeat process after time-lapse completion", "D. 1. Establish micron-scale photoactivation regions beneath individual cells using software control\n2. Photoactivate selected regions by opening digital diaphragm shutter for 1-1.5 seconds after acquiring 10 time points\n3. Start time-lapse acquisition with 80 time points at 5-second intervals\n4. Select new field of cells and repeat process after time-lapse completion"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/56655/clip_5.mp4"}} |
| {"qid": "ExpVid_6376", "question": "Given the following step list，which step was not performed in the video?\n1. Remove supernatant after centrifugation\n2. Add serum-free medium with collagenase A\n3. Shake mixture for 12 hours\n4. Centrifuge resulting solution\n5. Resuspend cell pellet in 5 mL complete medium\n6. Seed cells onto 25 cm² tissue culture flasks in 5 mL complete medium\n7. Grow cells in humidified incubator at 37°C with 5% CO₂", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7"], "answer": "E", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/4422/clip_4_removed_step_5.mp4"}} |
| {"qid": "ExpVid_5730", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Culture HCT116 cell line in DMEM cell culture medium\n2. Incubate cultures until six to twelve confluent plates are obtained\n3. Wash cells gently with 10 milliliters of PBS to remove cell culture medium\n4. Add three milliliters of ice-cold PBS supplemented with five-millimolar EDTA\n5. Incubate plates for five minutes at room temperature\n6. Detach cells from plates using a cell scraper\n7. Collect cells in 50-milliliter tube placed on ice\n8. Centrifuge at 250 G for 10 minutes\n9. Measure volume of harvested cells using tube's volumetric scale\n10. Resuspend harvested cell pellet in equal volume of lysis buffer\n11. Lyse cells by sonication\n12. Spin sample at 100,000 G for one hour at 4°C in ultracentrifuge rotor\n13. Remove cleared lysate using syringe without disturbing pellet or floating layer\n14. Add 2 microliters of cleared lysate into tube\n15. Add 18 microliters of water to tube\n16. Add 5 microliters of 5X SDS sample buffer to tube\n17. Add 1 mM GTP and 20 µM paclitaxel to collected supernatant\n18. Incubate mixture at 37°C for 30 minutes\n19. Prepare cushion buffer by adding 600 µL glycerol to 400 µL lysis buffer\n20. Supplement cushion buffer with 20 µM paclitaxel\n21. Prewarm cushion buffer to 37°C\n22. Add 800 microliters of cushion buffer to an ultracentrifuge tube\n23. Pipette GTP-paclitaxel-supplemented lysate carefully on top of cushion buffer\n24. Place tube in ultracentrifuge rotor\n25. Centrifuge at 100,000 G at 30°C for 30 minutes\n26. Mark outward-facing edge of tube to identify microtubule pellet location\n27. Add lysis buffer next to pellet\n28. Rotate tube several times to remove glycerol from pellet and tube walls\n29. Aspirate and repeat glycerol washing step three times\n30. Resuspend washed pellet gently using cut pipette tip in 50 microliters prewarmed resuspension buffer\n31. Place tube at 37 degrees\nAnswer with the step number only.", "options": null, "answer": "11", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/65126/clip_3_prediction.mp4"}} |
| {"qid": "ExpVid_7540", "question": "The study concludes that a low-cytotoxicity _____-based, two-dimensional FACS gating (_____ vs _____) with _____ DNA-content discrimination enables high-purity live isolation of stage-resolved spermatocytes and spermatids from adult mouse testes.", "options": null, "answer": "DyeCycle Violet (DCV) | DCV blue | DCV red | 1C/2C/4C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/61666_.mp4"}} |
| {"qid": "ExpVid_5101", "question": "What is the correct sequence of steps for the experimental procedure of heating and initial separation of hdcat-HKUST?", "options": ["A. 1. Ensure vial is tightly sealed\n2. Place sealed vial in oven at 70°C overnight\n3. Remove vial from oven\n4. Transfer vial to glove box with 15-mL centrifuge tube\n5. Remove centrifuge tube from glove box\n6. Transfer vial contents to centrifuge tube using anhydrous chloroform\n7. Centrifuge at 3,354×g for 1 minute to separate coated material\n8. Return centrifuge tube to glove box\n9. Extract supernatant with dropper and store in clean 40-mL glass vial", "B. 1. Ensure vial is tightly sealed\n2. Place sealed vial in oven at 70°C overnight\n3. Remove vial from oven\n4. Transfer vial to glove box with 15-mL centrifuge tube\n5. Transfer vial contents to centrifuge tube using anhydrous chloroform\n6. Remove centrifuge tube from glove box\n7. Centrifuge at 3,354×g for 1 minute to separate coated material\n8. Return centrifuge tube to glove box\n9. Extract supernatant with dropper and store in clean 40-mL glass vial", "C. 1. Ensure vial is tightly sealed\n2. Place sealed vial in oven at 70°C overnight\n3. Remove vial from oven\n4. Transfer vial to glove box with 15-mL centrifuge tube\n5. Transfer vial contents to centrifuge tube using anhydrous chloroform\n6. Remove centrifuge tube from glove box\n7. Centrifuge at 3,354×g for 1 minute to separate coated material\n8. Extract supernatant with dropper and store in clean 40-mL glass vial\n9. Return centrifuge tube to glove box", "D. 1. Ensure vial is tightly sealed\n2. Place sealed vial in oven at 70°C overnight\n3. Remove vial from oven\n4. Transfer vial contents to centrifuge tube using anhydrous chloroform\n5. Transfer vial to glove box with 15-mL centrifuge tube\n6. Remove centrifuge tube from glove box\n7. Centrifuge at 3,354×g for 1 minute to separate coated material\n8. Return centrifuge tube to glove box\n9. Extract supernatant with dropper and store in clean 40-mL glass vial"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/58052/clip_2.mp4"}} |
| {"qid": "ExpVid_6789", "question": "Given the following step list，which step was not performed in the video?\n1. Transfer L3 larvae using brush to borosilicate glass dish containing PBS\n2. Stir larvae in PBS to detach fly food\n3. Transfer larvae to well containing culture medium", "options": ["A. 1", "B. 2", "C. 3"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/61954/clip_1_removed_step_1.mp4"}} |
| {"qid": "ExpVid_7709", "question": "Based on the experiment, the main methodological advance is a low-cost ______ that enables ______ in-soil visualization and quantification of root plasticity, validated by higher ______ in ______ across multiple maize genotypes, without specialized equipment.", "options": null, "answer": "rhizobox protocol | repeated, non-invasive | root length density | nitrogen 15 labeled nutrient patches", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/58674_.mp4"}} |
| {"qid": "ExpVid_7408", "question": "In this study, CNT vias grown at ____ with a ____ catalyst exhibited ____ in ____ measurements, indicating ohmic contact between the CNT bundles and the Ti/TiN metal electrodes.", "options": null, "answer": "350°C | Co | linear I-V characteristics | 4-point-probe tests", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/53260_.mp4"}} |
| {"qid": "ExpVid_6179", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Generate extrachromosomal array with translational construct of target genes\n2. Obtain transgenic lines from Caenorhabditis Genetic Center or published research laboratory\n3. Expose worms to gamma radiation to integrate extrachromosomal lines into genome\n4. Select stably expressed lines showing marker protein expression (e.g., GFP or roller)\n5. Outcross lines at least two times to remove radiation-induced mutations\n6. Streak OP50 clone on agar plate\n7. Culture streaked bacteria in liquid LB broth overnight\n8. Seed NGM plates with overnight liquid OP50 culture\n9. Incubate seeded plates at 37°C overnight to grow bacterial lawn\n10. Cool down incubated plates at room temperature for at least 30 minutes\n11. Grow transgenic worm line at 20°C without starvation for ≥2 generations\n12. Pick 8-10 young gravid adults to new worm plate\n13. Allow adults to lay eggs for 3-5 hours\n14. Remove all adults from plates\n15. Synchronize worms by letting eggs hatch and grow larvae for ≈48 hours to L4 stage\n16. Heat-stress worms by incubating plates with L4 larvae at 35°C for 2-5 hours\n17. Place thermometer in incubator to monitor temperature\n18. Pipette 10 microliters of M9 buffer onto center of glass slide\n19. Pick heat-shocked worms\n20. Transfer worms into M9 buffer drop on slide\n21. Wash worms off plate using M9 buffer (alternative method)\n22. Centrifuge sample at 1000 × g at room temperature for 1 minute\n23. Discard supernatant\n24. Add worms to glass slide\n25. Add worms to glass slide\n26. Drain excess liquid using soft tissue under dissecting microscope\n27. Add 10 microliters of 95% alcohol onto worms under microscope while observing\n28. Repeat ethanol addition twice after previous ethanol dries\n29. Add 10 microliters of DAPI to worms\n30. Add 10 microliters of DAPI to the worms\n31. Apply cover slip to slide\n32. Seal slide w\nAnswer with the step number only.", "options": null, "answer": "75", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/57914/clip_11_prediction.mp4"}} |
| {"qid": "ExpVid_4632", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Cut hole in bottom of one-ounce cup\n2. Glue screen over hole for air exchange\n3. Aspirate insect colony into 15 milliliter conical tube\n4. Chill insects on ice briefly\n5. Identify female insects by presence of ovipositor on ventral abdomen\n6. Transfer identified females into prepared cup\n7. Seal cup with 5x5 cm paraffin wax film after collecting 15 females\n8. Apply 400 microliters of 10% sucrose solution to top of film\n9. Place second 5x5 cm paraffin wax film over sucrose solution\n10. Place adult chamber on egg collection dish with plastic paraffin wax film contacting oviposition medium\n11. Wrap entire egg-laying chamber with plastic wrap while avoiding air holes\n12. Place chamber at 25°C with 70% humidity and 14-hour light/10-hour dark cycle\n13. Apply 1x15 mm double-sided tape strip onto 22x30 mm cover slip\n14. Place cover slip onto oviposition medium of egg-laying chamber\n15. Transfer individual semi-transparent embryos from agar surface to double-sided tape using fine brush under dissecting microscope\n16. Arrange approximately 25 banana-shaped embryos laterally on tape with larger ends adhered\n17. Place cover slip onto 100 × 15 ml Petri dish containing 1% agar\n18. Position setup under dissecting microscope inside humidified hood\n19. Place tip of quartz injection needle in water drop\n20. Initiate injection cycle for pressure calibration\n21. Insert needle into larger end of embryo approaching from left side of cover slip\n22. Deliver injection solution into egg\n23. Withdraw needle from embryo\n24. Transfer cover slip to new 1% agar dish surface\n25. Place dish into humidity chamber\n26. Transfer surviving embryos using fine brush to 35x10mm Petri dish containing water-moistened filter paper\n27. Seal Petri dish with plastic paraffin wax film\n28. Place sealed Petri dish in 25°C incubator\n29. Transfer emergent first instar nymphs using fine brush to Petri dish containing leaf clippi\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "1, 2, 3, 4, 5, 6, 7, 8, 9", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/62417/clip_1.mp4"}} |
| {"qid": "ExpVid_4887", "question": "What is the correct sequence of steps for the enzymatic dissociation and incubation of optic lobes?", "options": ["A. 1. Remove 1x RS from each sample\n2. Add 300 microliters of dissociation solution to optic lobes\n3. Incubate samples at 25°C and 1,000 RPM for 15 minutes in microtube ThermoMixer\n4. Stop ThermoMixer at 5-minute incubation time point\n5. Pipette up 200 microliters of solution and mix\n6. Allow lobes to sink to bottom of microtube\n7. Wait for lobes to settle after 15-minute incubation\n8. Remove dissociation solution from lobes without disturbing tissue", "B. 1. Remove 1x RS from each sample\n2. Add 300 microliters of dissociation solution to optic lobes\n3. Stop ThermoMixer at 5-minute incubation time point\n4. Allow lobes to sink to bottom of microtube\n5. Pipette up 200 microliters of solution and mix\n6. Incubate samples at 25°C and 1,000 RPM for 15 minutes in microtube ThermoMixer\n7. Wait for lobes to settle after 15-minute incubation\n8. Remove dissociation solution from lobes without disturbing tissue", "C. 1. Remove 1x RS from each sample\n2. Add 300 microliters of dissociation solution to optic lobes\n3. Incubate samples at 25°C and 1,000 RPM for 15 minutes in microtube ThermoMixer\n4. Stop ThermoMixer at 5-minute incubation time point\n5. Allow lobes to sink to bottom of microtube\n6. Pipette up 200 microliters of solution and mix\n7. Wait for lobes to settle after 15-minute incubation\n8. Remove dissociation solution from lobes without disturbing tissue", "D. 1. Remove 1x RS from each sample\n2. Add 300 microliters of dissociation solution to optic lobes\n3. Incubate samples at 25°C and 1,000 RPM for 15 minutes in microtube ThermoMixer\n4. Wait for lobes to settle after 15-minute incubation\n5. Remove dissociation solution from lobes without disturbing tissue\n6. Stop ThermoMixer at 5-minute incubation time point\n7. Allow lobes to sink to bottom of microtube\n8. Pipette up 200 microliters of solution and mix"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/58474/clip_6.mp4"}} |
| {"qid": "ExpVid_4422", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Place eight-day-old specific pathogen-free embryonated chicken eggs in rotating egg tray with stamped ends facing upward\n2. Set rotating tray inside egg incubator set to 36°C with 50% humidity\n3. Incubate eggs for 48 hours\n4. Place incubated eggs in egg rack with stamped ends up\n5. Transfer egg rack to laminar flow hood\n6. Turn off room and hood lights\n7. Hold stamped end of egg lightly to egg candler to expose vasculature of chorioallantoic membrane (CAM) and air sack\n8. Make a mark between two major blood vessels with a pencil\n9. Make a hole at egg tip above air sack at stamped end using sterile push pin (3mm deep)\n10. Make a second hole at pencil mark using sterile push pin (3mm deep)\n11. Squeeze air from safety bulb\n12. Press safety bulb firmly against hole above air sack\n13. Slowly release bulb to apply suction at pencil mark hole\n14. Confirm air sack displacement to pencil mark using candler\n15. Make both holes deeper if air sack displacement unsuccessful\n16. Deepen both holes slightly\n17. Reapply suction using safety bulb\n18. Hold egg with one hand and position rotary tool with 1.5-1.6 inch cutoff wheel attachment\n19. Make two transverse cuts (2 cm length, 1 cm apart) through shell without touching CAM\n20. Make longitudinal cuts at ends of transverse cuts by lightly touching cutoff wheel to shell\n21. Slide sterile forceps under and parallel to shell piece\n22. Grab shell piece with forceps and cleanly remove it\n23. Place basement membrane protein mixture (e.g., matrigel) on ice to prevent polymerization\n24. Use 4-5 ml of 2 mM EDTA solution per flask to detach tumor cells\n25. Place flask in tissue culture incubator for 5 minutes\n26. Resuspend detached cells in medium\n27. Count cell number\n28. Transfer volume required for 500,000-2 million cells to new tube\n29. Centrifuge tube at 500 × g for 5 minutes\n30. Discard supernatant\n31. Resuspend cell pellet in 40 μl PBS containin\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "8, 9, 10, 11, 12, 13, 14, 15", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/52411/clip_2.mp4"}} |
| {"qid": "ExpVid_4533", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Collect green coffee fruits (120-150 days after flowering) from plantations\n2. Select approximately 300 uniformly-sized healthy fruits\n3. Withdraw peduncles from selected fruits\n4. Dip fruits in 2% soap solution and clean thoroughly by rubbing\n5. Rinse fruits three times with clean water\n6. Immerse fruits in 0.5% sodium hypochlorite solution\n7. Place immersed fruits on shaker at 110 rpm for 10 minutes\n8. Rinse fruits three times with clean water\n9. Dry fruits using sterile paper towels\n10. Place fruits 55 cm from UV source in UV-enabled horizontal laminar flow station for 15-minute irradiation\n11. Move fruits every five minutes during irradiation to ensure whole-surface exposure\n12. Immerse newly emerged coffee berry borers in 0.5% sodium hypochlorite solution\n13. Agitate insects slowly with brush for 10 minutes\n14. Filter insects through muslin cloth\n15. Wash insects three times with sterile distilled water\n16. Remove excess water using sterile paper towels\n17. Prepare group of 30 green coffee fruits\n18. Prepare 30 green coffee fruits per experimental unit\n19. Place prepared fruits in plastic box\n20. Apply test product concentrations using portable sprayer unit\n21. Spray control group of green coffee fruits with water\n22. Spray experimental units sequentially with at least three units per treatment\n23. Release two coffee berry borer adults in sterile hood\n24. Release two disinfected coffee berry borer adults per green coffee fruit in sterile hood\n25. Cover boxes after 30 minutes\n26. Transfer plastic boxes with infested fruits to dark incubator/room with controlled conditions\n27. Count number of borer-infested fruits and living/dead insects outside fruits in each box after specified days\n28. Dissect each green coffee fruit under stereo microscope at 10x magnification\n29. Count number of healthy seeds and insect-damaged seeds per fruit\n30. Count different coffee berry borer bio\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "8, 9, 10, 11", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/63694/clip_4.mp4"}} |
| {"qid": "ExpVid_5114", "question": "What is the correct sequence of steps for the positive photoresist application experimental procedure?", "options": ["A. 1. Execute spin coater program: 500 rpm with 3,000 rpm/s acceleration for 10 seconds, then 4,000 rpm with 3,000 rpm/s acceleration for 30 seconds\n2. Deposit positive photoresist onto wafer using dropper to cover 75% of surface area\n3. Place wafer on spin coater", "B. 1. Deposit positive photoresist onto wafer using dropper to cover 75% of surface area\n2. Place wafer on spin coater\n3. Execute spin coater program: 500 rpm with 3,000 rpm/s acceleration for 10 seconds, then 4,000 rpm with 3,000 rpm/s acceleration for 30 seconds", "C. 1. Place wafer on spin coater\n2. Deposit positive photoresist onto wafer using dropper to cover 75% of surface area\n3. Execute spin coater program: 500 rpm with 3,000 rpm/s acceleration for 10 seconds, then 4,000 rpm with 3,000 rpm/s acceleration for 30 seconds", "D. 1. Place wafer on spin coater\n2. Execute spin coater program: 500 rpm with 3,000 rpm/s acceleration for 10 seconds, then 4,000 rpm with 3,000 rpm/s acceleration for 30 seconds\n3. Deposit positive photoresist onto wafer using dropper to cover 75% of surface area"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61013/clip_6.mp4"}} |
| {"qid": "ExpVid_5110", "question": "What is the correct sequence of steps for configuring multidimensional imaging software for DAPI/GFP/DIC acquisition?", "options": ["A. 1. Open microscope-provided software\n2. Click 'Camera' option\n3. Click 'Acquisition' menu\n4. Select microscope-connected camera\n5. Click 'Multidimensional Acquisition' option\n6. Click multi-channel button\n7. Select blue, green, and DIC channels\n8. Enable blue DAPI, green GFP, and gray DIC channels\n9. Right-click to close red Rhodamine and white Brightfield channels", "B. 1. Open microscope-provided software\n2. Click 'Acquisition' menu\n3. Click 'Camera' option\n4. Select microscope-connected camera\n5. Click 'Multidimensional Acquisition' option\n6. Click multi-channel button\n7. Right-click to close red Rhodamine and white Brightfield channels\n8. Select blue, green, and DIC channels\n9. Enable blue DAPI, green GFP, and gray DIC channels", "C. 1. Open microscope-provided software\n2. Click 'Acquisition' menu\n3. Click 'Camera' option\n4. Select microscope-connected camera\n5. Click 'Multidimensional Acquisition' option\n6. Click multi-channel button\n7. Select blue, green, and DIC channels\n8. Enable blue DAPI, green GFP, and gray DIC channels\n9. Right-click to close red Rhodamine and white Brightfield channels", "D. 1. Open microscope-provided software\n2. Click 'Acquisition' menu\n3. Click 'Multidimensional Acquisition' option\n4. Click 'Camera' option\n5. Select microscope-connected camera\n6. Click multi-channel button\n7. Enable blue DAPI, green GFP, and gray DIC channels\n8. Select blue, green, and DIC channels\n9. Right-click to close red Rhodamine and white Brightfield channels"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57914/clip_10.mp4"}} |
| {"qid": "ExpVid_4294", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Place female rat on dissection table\n2. Hold ankle firmly between thumb, index finger, and middle finger\n3. Sever calcaneal tendon using 12-centimeter straight blunt scissors\n4. Make incisions through muscle layers near middle of back of leg using fine forceps and scissors until sciatic nerve is exposed\n5. Moisturize cavity using ice-cold modified Krebs-Henseleit buffer (MKHB) to prevent nerve drying\n6. Pull flaps of skin on each side using hemostats\n7. Maintain incision open for finer dissection\n8. Interrupt muscle on medial side of leg using fine scissors\n9. Free nerve from surrounding tissues\n10. Maintain moisture in area with ice-cold MKHB\n11. Dissect overlying muscle tissue while exposing nerve moving up leg\n12. Free nerve from connective tissues nearer to spine\n13. Gently pull end of nerve near ankle using forceps\n14. Severe nerve close to spine using fine scissors\n15. Place dissected nerve in 15-mL centrifuge tube filled with MKHB\n16. Place tube on ice until cleaning procedure\n17. Fill coated Petri dish halfway with chilled oxygenated MKHB\n18. Place dissected sciatic nerve in Petri dish\n19. Pin both ends of nerve to dish ensuring straight alignment without kinks/twists\n20. Tie double knot with 6-0 silk sutures around each nerve end to prevent cytosol leakage\n21. Position knots adjacent to insect pins near nerve center to prevent leakage\n22. Under microscope, remove fat/blood vessels/muscle tissue from nerve using 2mm angled spring scissors and fine forceps\n23. Prune unused nerve branches\n24. Replace buffer with fresh chilled oxygenated MKHB every 5 minutes during cleaning\n25. Transfer cleaned nerve to transport tube filled with buffer\n26. Place transport tube on ice\n27. Prepare clean dual-chamber nerve bath\n28. Position nerve bath below level of two-liter bottle on heating stirrer\n29. Connect bath drain to peristaltic pump inlet\n30. Connect peristaltic pump outlet to tu\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "1, 2, 3, 4, 5", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/63838/clip_1.mp4"}} |
| {"qid": "ExpVid_5664", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Isolate RNA using a method that preserves small RNAs\n2. Resuspend isolated RNA at concentration ≥2 mg/ml\n3. Mix 25 μg total RNA with 0.5 μg 5'P-Cy3-dye in 20 μl reaction volume\n4. Add mixture to 1X HEPs buffer\n5. Supplement reaction with T4 RNA Ligase 1D TT and ATP\n6. Scale down RNA amount and reaction volume if less RNA is available\n7. Add carrier (e.g., yeast tRNA) if RNA is dilute to aid precipitation\n8. Incubate reaction on ice in refrigerator for 2-24 hours\n9. Add sodium acetate to 0.3 M final concentration\n10. Add three volumes of ethanol\n11. Precipitate RNA overnight at -20°C\n12. Pre-hybridize slides in filtered solution (3X SSC, 0.1% SDS, 0.2% BSA) at 37°C for 30-60 minutes\n13. Submerge slides in water several times\n14. Submerge slides in isopropanol\n15. Dry slides using centrifuge with slide adapter (100xg, 5 minutes, 22°C)\n16. Rinse lifter slips in water\n17. Rinse lifter slips in ethanol\n18. Air-dry lifter slips\n19. Place slide in microarray hybridization chamber base\n20. Position lifter slip on slide to cover probe area and white strips\n21. Contact slide in dark\n22. Spin down precipitated labeled RNA\n23. Wash RNA pellet once with 70% ethanol\n24. Air dry RNA pellet\n25. Prepare hybridization solution using 1/15 to 1/100 volume purified labeled reference DNA per RNA sample\n26. Dissolve RNA pellet completely with approximately 60 microliters hybridization solution\n27. Add 20 microliters water to each sample during incubation\n28. Add 20 microliters of water to each humidifying well in microarray hybridization chamber base\n29. Add mixture of labeled RNA and reference DNA to slide\n30. Use thin pipette tip to touch edge of lifter slip and apply suction to draw solution into space between slip and slide\n31. Place hybridization chamber cover over base\n32. Seal chamber with metal clips\n33. Place entire ch\nAnswer with the step number only.", "options": null, "answer": "50", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/3250/clip_8_prediction.mp4"}} |
| {"qid": "ExpVid_5968", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Drill one 4 mm diameter hole 20 mm from each end of 1200 × 30 × 4.5 mm acrylic diffuser bar\n2. Drill and tap threaded holes 20 mm from each end of aluminum u-bar section\n3. Drill and tap threaded holes into base of aluminum u-bar to suit mounting hardware\n4. Straighten one 1.25m length of 1.25mm bare copper wire by securing one end to vise\n5. Secure opposite end of wire within grips of hand drill\n6. Turn on hand drill at low speed\n7. Repeat wire straightening process for second wire\n8. Use fine tip permanent marker to mark photodiode positions along diffuser edge\n9. Mark first photodiode position at 13.5cm from one diffuser end\n10. Mark subsequent photodiode positions every 2cm toward far diffuser end\n11. Center photodiode on diffuser bar with electrical tabs pointing sideways\n12. Place wire under one tab to mark first copper wire position\n13. Mark wire position at center and opposite end of diffuser bar\n14. Secure first straightened copper wire to diffuser along marks using cyanoacrylate glue\n15. Glue 50 photodiodes facedown at 20mm intervals ensuring center alignment and uniform orientation (large tab on wire, small tab opposite)\n16. Place second copper wire under smaller tabs of photodiodes\n17. Secure wire to diffuser with cyanoacrylate glue\n18. Apply solder flux to both tabs of one photodiode, adjacent wires, and underlying wires\n19. Solder each diode tab to underlying copper wires using fine-tipped soldering iron at 350-400°C\n20. Shine light onto each photodiode to test solder connections\n21. Check voltage signal for each diode across wires using multimeter\n22. Solder 1.5 ohm low-temperature coefficient precision resistor in parallel across copper wires (optional)\n23. Solder male end of waterproof DC connector to ends of copper wires\n24. Seal connections using glue-lined heat shrink tubing\n25. Apply \nAnswer with the step number only.", "options": null, "answer": "31", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/59447/clip_6_prediction.mp4"}} |
| {"qid": "ExpVid_6093", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Dissolve 0.3 grams of polystyrene-block-polyethylene oxide copolymer (SEO) in anhydrous N-methyl-2-pyrrolidone (NMP) with dry lithium bis(trifluoromethanesulfonyl)imide (LITFSI) salt\n2. Use LITFSI salt to SEO mass ratio of 0.275 and NMP to SEO mass ratio of 13.13 during dissolution\n3. Heat solution to 90 degrees Celsius on hot plate\n4. Heat solution to 90°C on hot plate\n5. Stir solution until polymer dissolves\n6. Cast polymer and salt mixture onto 15x15 cm nickel foil\n7. Spread mixture using doctor blade loosely\n8. Cover film with aluminum foil\n9. Dry film on casting plate at 60°C overnight\n10. Peel film from nickel foil\n11. Dry film under vacuum at 90°C\n12. Wrap freestanding film in nickel foil\n13. Store film in airtight box inside glove box\n14. Cut two lithium metal electrodes using 7/16-inch diameter metal punch from 99.9% pure lithium metal foil roll\n15. Cut polymer electrolyte film using half-inch diameter metal punch\n16. Sandwich polymer electrolyte film between two lithium metal electrodes\n17. Press nickel tabs onto lithium electrodes\n18. Vacuum seal assembled cell in polypropylene/nylon-lined aluminum pouch\n19. Place vacuum-sealed sample into 90°C oven\n20. Place vacuum-sealed sample into oven held at 90°C\n21. Apply current density of 0.175 mA/cm² through sample for 4 hours\n22. Allow sample to rest for 45 minutes\n23. Apply current density of -0.175 mA/cm² through sample for 4 hours\n24. Allow sample to rest for 45 minutes\n25. Bring cycled symmetric cell into glove box\n26. Remove cell from its pouch\n27. Use 1/8 inch metal punch to cut center portion of cell\n28. Seal center portion in pouch material\n29. Remove sealed sample from glove box for transport\n30. Affix sample to stage using polyamide tape\n31. Place metal marker at sample center for rotation reference\n32. Mount sample onto rotating stage\n33. \nAnswer with the step number only.", "options": null, "answer": "13", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/53021/clip_2_prediction.mp4"}} |
| {"qid": "ExpVid_6628", "question": "Given the following step list，which step was not performed in the video?\n1. Clamp the common hepatic duct\n2. Pierce microinjector into distal biliopancreatic duct retrogradely\n3. Fix needle tip near duodenal papilla with clamp\n4. Infuse 10 microliters of 5% sodium taurocholate into biliopancreatic duct at 5 microliters/minute\n5. Maintain biliopancreatic duct closure for 5 minutes\n6. Remove clamps", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/63129/clip_6_removed_step_4.mp4"}} |
| {"qid": "ExpVid_4339", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Add 1 mL of ice-cold 0.9% sodium chloride to 1.5-mL tube containing larvae\n2. Close tube lid\n3. Vertically flip tube to wash larvae\n4. Place tube on ice for 30 seconds\n5. Remove sodium chloride solution using 1-mL pipette\n6. Centrifuge samples at 2,000 g for 1 minute at 4°C\n7. Remove residual solution using 200-μL pipette and immediately freeze sample in liquid nitrogen\n8. Label side of 2-mL screw-cap bead tube with ethanol-proof marker\n9. Tare mass of labeled bead tube using analytical balance (0.01 mg accuracy)\n10. Remove 1.5-mL sample tube from liquid nitrogen dewar using long forceps\n11. Grab frozen tube while wearing nitrile gloves and invert it\n12. Sharply pound inverted tube lid against benchtop to dislodge frozen pellet\n13. Pour dislodged pellet into pre-tared 2-mL screw-cap bead tube\n14. Measure combined mass of larval pellet and bead tube\n15. Place sample tube into liquid nitrogen\n16. Place sample tubes in -20°C benchtop cooler\n17. Add 0.8 mL pre-chilled 90% methanol containing 2 μg/mL succinic-D4 acid to each sample tube\n18. Return samples to -20°C benchtop cooler\n19. Add 0.8 mL pre-chilled 90% methanol containing 2 μg/mL succinic-D4 acid to empty bead tube for negative control\n20. Homogenize samples for 30 seconds at 6.45 meters per second using bead mill homogenizer in 4°C temperature control room\n21. Return homogenized samples to -20°C benchtop cooler\n22. Transfer cooler to -20°C freezer for at least one hour\n23. Centrifuge tubes at 20,000 g for 5 minutes at 4°C to remove precipitate\n24. Transfer 600 microliters of supernatant to new 1.5-milliliter microcentrifuge tube without disturbing precipitate\n25. Open all sample tubes and place them in vacuum centrifuge\n26. Dry samples at room temperature until all solvent is removed\n27. Prepare solution of 40 mg/mL methoxylamine hydrochloride (MOX) in anhydrous pyridine\n28. Store MOX and anhydrous pyridine in desiccator\n2\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "23, 24, 25, 26", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57847/clip_5.mp4"}} |
| {"qid": "ExpVid_4867", "question": "What is the correct sequence of steps for the experimental procedure 'Irrigation and cavity formation'?", "options": ["A. 1. Arrange slow irrigation of substrate with 150 milliliters of water using drip emitters or manual method\n2. Remove spacers to create empty cavities for patches\n3. Let substrate rest several hours or overnight for water diffusion and thorough wetting around spacers", "B. 1. Let substrate rest several hours or overnight for water diffusion and thorough wetting around spacers\n2. Arrange slow irrigation of substrate with 150 milliliters of water using drip emitters or manual method\n3. Remove spacers to create empty cavities for patches", "C. 1. Arrange slow irrigation of substrate with 150 milliliters of water using drip emitters or manual method\n2. Let substrate rest several hours or overnight for water diffusion and thorough wetting around spacers\n3. Remove spacers to create empty cavities for patches", "D. 1. Remove spacers to create empty cavities for patches\n2. Arrange slow irrigation of substrate with 150 milliliters of water using drip emitters or manual method\n3. Let substrate rest several hours or overnight for water diffusion and thorough wetting around spacers"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/58674/clip_6.mp4"}} |
| {"qid": "ExpVid_7280", "question": "In the operant task measuring social motivation with a progressively increasing response requirement, ________ mice that were ________ exhibited a significantly ________ compared with similarly housed ________ mice.", "options": null, "answer": "BTBR | group-housed | lower breakpoint | C57BL/6 (B6)", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/53009_.mp4"}} |
| {"qid": "ExpVid_4869", "question": "What is the correct sequence of steps for bead drying and quality check?", "options": ["A. 1. Decant ethanol from container\n2. Dry beads and sprinkle to form thin layer in sterile container\n3. Gently tap or shake container to check for sandy texture without clumping/flaking\n4. Place open container in biosafety cabinet for overnight air-drying", "B. 1. Decant ethanol from container\n2. Place open container in biosafety cabinet for overnight air-drying\n3. Dry beads and sprinkle to form thin layer in sterile container\n4. Gently tap or shake container to check for sandy texture without clumping/flaking", "C. 1. Decant ethanol from container\n2. Dry beads and sprinkle to form thin layer in sterile container\n3. Place open container in biosafety cabinet for overnight air-drying\n4. Gently tap or shake container to check for sandy texture without clumping/flaking", "D. 1. Decant ethanol from container\n2. Gently tap or shake container to check for sandy texture without clumping/flaking\n3. Dry beads and sprinkle to form thin layer in sterile container\n4. Place open container in biosafety cabinet for overnight air-drying"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/62559/clip_3.mp4"}} |
| {"qid": "ExpVid_7156", "question": "Using this live-tissue imaging protocol, dynamic reorganization of the ____ and ____ can be directly observed in dividing ____ within intact ____.", "options": null, "answer": "endoplasmic reticulum | microtubules | spermatocytes | Drosophila testes", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/60961_.mp4"}} |
| {"qid": "ExpVid_5834", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Set up and label up to eight 500 microliter tubes\n2. Add up to 1 mg protein sample in up to 112 microliters volume to each tube\n3. Add 30 microliters of 5x sample buffer to each protein sample\n4. Add 8 microliters of 1M DTT to each protein sample\n5. Adjust each sample with water to final volume of 150 microliters\n6. Heat vials at 95°C for 5 minutes to denature samples\n7. Allow heated samples to cool to room temperature\n8. Remove gel free 8,100 cartridge from foil pouch\n9. Remove and discard plate sealer\n10. Remove storage buffer from cartridge compartments using pipette\n11. Invert cartridge to drain storage buffer if all eight chambers are used\n12. Add 8 milliliters of gel-free running buffer to each anode buffer reservoir\n13. Add 6 milliliters of gel-free running buffer to each cathode buffer reservoir\n14. Pipette 100 microliters of gel-free running buffer into each collection chamber\n15. Using an 8-channel pipetter (100-200 μL), remove and discard buffer overflow from sample loading chambers\n16. Load denatured samples into loading chambers using 8-channel pipetter\n17. Place cartridge into GelFree 8,100 fractionation station\n18. Lower electrode arrays and close station lid\n19. Navigate to list of pre-programmed methods using touchscreen GUI\n20. Press method button to select appropriate pre-programmed method\n21. Press retrieve button and enter method number using onscreen keypad\n22. Press apply button to load selected method\n23. Press channel button and select channels for run\n24. Press done button to confirm channel selection\n25. Ensure safety lid is closed with green indicator light\n26. Press start button to begin experiment\n27. Open lid of instrument\n28. Use innate channel pipette to transfer 150 microliters of liquid from collection chambers to collection tubes or multi-well plate\n29. Add 100 microlit\nAnswer with the step number only.", "options": null, "answer": "35", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/1842/clip_7_prediction.mp4"}} |
| {"qid": "ExpVid_6124", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Place autoclaved surgical instruments on surgical table\n2. Shave abdomen of anesthetized rat\n3. Position rat in supine position on surgical board\n4. Clean abdomen with povidone iodine\n5. Clean abdomen with 70% ethanol\n6. Apply ophthalmic ointment under rat's eyes to prevent dryness\n7. Secure rat in place on surgical board\n8. Start data acquisition software and begin recording\n9. Turn on ventilator at 4 milliliters per kilogram\n10. Ensure positive end expiratory pressure is 2 centimeters of water\n11. Perform midline laparotomy using scissors from xiphoid process to pubic symphysis\n12. Perform medial-lateral visceral rotation using blunt instrument and visualize infrahepatic inferior vena cava\n13. Inject heparin into inferior vena cava using 20-gauge needle\n14. Cut skin from sternal notch to below mandible angle using scissors\n15. Dissect toward trachea\n16. Bluntly dissect away necessary strap muscles\n17. Expose trachea\n18. Make transverse incision on anterior trachea between cartilaginous rings\n19. Place 5-0 silk suture around trachea\n20. Insert endotracheal tube into cartilaginous rings\n21. Secure endotracheal tube with 5-0 silk suture\n22. Connect endotracheal tube to ventilator\n23. Ensure proper chest rising\n24. Perform median sternotomy using scissors to enter thoracic cavity\n25. Place chest wall retractors to expose heart and lungs while avoiding lung manipulation\n26. Remove thymus from anterior mediastinum using sharp and blunt dissection\n27. Identify pulmonary artery and place 5-0 silk suture around it for cannulation preparation\n28. Make 2-3 mm incision in right ventricular outflow tract using scissors\n29. Place arterial cannula within pulmonary artery and secure with 5-0 suture\n30. Euthanize the rat\n31. Connect de-aired lung preservation fluid to arterial cannula\n32. Gravity flush lungs via arteria\nAnswer with the step number only.", "options": null, "answer": "29", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/201866/clip_4_prediction.mp4"}} |
| {"qid": "ExpVid_5984", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Flush fixative line with buffer using 50 milliliter syringe repeatedly\n2. Clear fixative line until all air bubbles are removed\n3. Place outlet valve into beaker filled with buffer\n4. Turn exit valve off\n5. Remove tube from syringe while squeezing it\n6. Ensure drop of buffer hangs from tube end\n7. Place filled fixative inlet tubing into bottle containing 200 milliliters of paraldehyde fixative per animal\n8. Prevent introduction of air bubbles during tubing placement\n9. Tighten cap while ensuring seal is properly seated\n10. Place bottle into perfusion rig\n11. Close outlet port\n12. Turn buffer valve to same position as fixative valve\n13. Open outlet port and clear buffer line\n14. Place tube into buffer bottle\n15. Pump rubber manometer bulb to test pressure resistance\n16. Fill perfusion needle with buffer to prevent air introduction\n17. Place fully anesthetized rat on shallow tray filled with crushed ice\n18. Make 5-6 cm lateral incision through integument and abdominal wall beneath rib cage\n19. Separate liver from diaphragm\n20. Make small incision in diaphragm using curved blunt scissors\n21. Continue diaphragm incision along entire length of rib cage to expose pleural cavity\n22. Place curved blunt scissors along one side of ribs while carefully displacing lungs\n23. Cut through rib cage up to collarbone\n24. Make cut on contralateral side\n25. Lift sternum away\n26. Trim tissue connecting sternum to heart\n27. Clamp tip of sternum with hemostat\n28. Place hemostat over head\n29. Make small incision to posterior end of left ventricle using iris scissors\n30. Pass 15 gauge blunt or olive-tipped perfusion needle through incised ventricle into ascending aorta\n31. Clamp heart with hemostat to secure needle and prevent leakage\n32. Attach perfusion needle in right atrium to outlook port without introducing air bubbles\n33. \nAnswer with the step number only.", "options": null, "answer": "56", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/3564/clip_9_prediction.mp4"}} |
| {"qid": "ExpVid_4154", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Flame worm pick over Bunsen burner\n2. Scoop fresh E. coli from edge of bacterial lawn using pick\n3. Transfer scooped E. coli onto large-scale culture plate\n4. Pick single gravid adult from fourth chunk plate\n5. Add 5 microliters of freshly prepared alkaline hypochlorite solution to corner of plate away from E. coli lawn\n6. Place gravid adult into alkaline hypochlorite solution\n7. Tap nematode to disrupt cuticle and release eggs\n8. Place total of five adults evenly around E. coli lawn using same method\n9. Place lid back onto plate\n10. Pour 50 milliliters of M9 solution onto large-scale culture plate surface\n11. Swirl plate to ensure M9 covers entire nematode growth medium agarose surface\n12. Prime sterile serological pipette with M9\n13. Tilt plate to gather M9 and worm population in one corner\n14. Aspirate worm suspension using primed pipette and add to 50 milliliter conical tube\n15. Place tube on rocker to disrupt bacterial clumps and debris\n16. Transfer 15 milliliters of worms from each collection tube into three 15 milliliter conical tubes\n17. Sediment worms in tubes by centrifugation\n18. Carefully aspirate supernatants without disturbing worm pellets\n19. Add 13 milliliters of worm suspension to each tube\n20. Invert tubes to resuspend pellets and wash off bacteria/debris\n21. Centrifuge tubes again\n22. Wash worm pellets three times with 10 milliliters of fresh M9 solution per wash\n23. Dilute three 100 microliter aliquots of worm sample in 900 microliters of M9 solution per sample\n24. Make serial dilutions using the aliquots\n25. Place stock worm sample suspensions on a rocker for continuous movement\n26. Mix worm dilutions until homogeneous\n27. Add five microliters of solution from first dilution to glass microscope slide\n28. Count worms by light microscopy\n29. Average dilution counts after counting each aliquot replicate three times\n30. Split worm samples into experimental ali\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "23, 24, 25, 26, 27, 28, 29, 30, 31", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61453/clip_4.mp4"}} |
| {"qid": "ExpVid_5044", "question": "What is the correct sequence of steps for the brain exposure surgery procedure involving micro-dissection to remove cuticle and muscle 16, followed by extraction of air sacs?", "options": ["A. 1. Use sharpened very fine forceps to make two cuts at base of central dark cuticle triangle on each side of neck\n2. Select highest magnification for dissection\n3. Cut around dark triangle\n4. Remove part of cuticle from dark triangle region\n5. Use very fine forceps to carefully pinch top of area to sever muscle 16\n6. Verify cessation of rhythmic movement to confirm muscle 16 removal\n7. Starting from medial edge of dark triangle region, use forceps to cut remaining cuticle in small pieces\n8. Remove remaining cuticle pieces\n9. Grasp air sacs with forceps\n10. Slowly and steadily pull away one air sac at a time", "B. 1. Select highest magnification for dissection\n2. Use sharpened very fine forceps to make two cuts at base of central dark cuticle triangle on each side of neck\n3. Cut around dark triangle\n4. Use very fine forceps to carefully pinch top of area to sever muscle 16\n5. Verify cessation of rhythmic movement to confirm muscle 16 removal\n6. Remove part of cuticle from dark triangle region\n7. Starting from medial edge of dark triangle region, use forceps to cut remaining cuticle in small pieces\n8. Remove remaining cuticle pieces\n9. Grasp air sacs with forceps\n10. Slowly and steadily pull away one air sac at a time", "C. 1. Select highest magnification for dissection\n2. Use sharpened very fine forceps to make two cuts at base of central dark cuticle triangle on each side of neck\n3. Cut around dark triangle\n4. Remove part of cuticle from dark triangle region\n5. Use very fine forceps to carefully pinch top of area to sever muscle 16\n6. Starting from medial edge of dark triangle region, use forceps to cut remaining cuticle in small pieces\n7. Remove remaining cuticle pieces\n8. Verify cessation of rhythmic movement to confirm muscle 16 removal\n9. Grasp air sacs with forceps\n10. Slowly and steadily pull away one air sac at a time", "D. 1. Select highest magnification for dissection\n2. Use sharpened very fine forceps to make two cuts at base of central dark cuticle triangle on each side of neck\n3. Cut around dark triangle\n4. Remove part of cuticle from dark triangle region\n5. Use very fine forceps to carefully pinch top of area to sever muscle 16\n6. Verify cessation of rhythmic movement to confirm muscle 16 removal\n7. Starting from medial edge of dark triangle region, use forceps to cut remaining cuticle in small pieces\n8. Remove remaining cuticle pieces\n9. Grasp air sacs with forceps\n10. Slowly and steadily pull away one air sac at a time"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61876/clip_7.mp4"}} |
| {"qid": "ExpVid_7302", "question": "In coronal mouse brain sections at Bregma ____ mm, immunofluorescence showed WFA signals in the ____ and the ____, whereas AVP signals were observed in the ____.", "options": null, "answer": "-0.82 | perifornical area of the anterior hypothalamus | reticular nucleus | paraventricular nucleus", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/62876_.mp4"}} |
| {"qid": "ExpVid_6582", "question": "Given the following step list，which step was not performed in the video?\n1. Open lid of instrument\n2. Use innate channel pipette to transfer 150 microliters of liquid from collection chambers to collection tubes or multi-well plate\n3. Add 100 microliters of gel free running buffer per channel to collection chambers\n4. Pipette buffer up and down twice in collection chambers\n5. Add 100 microliters of gel free running buffer to collection chambers", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/1842/clip_6_removed_step_4.mp4"}} |
| {"qid": "ExpVid_6730", "question": "Given the following step list，which step was not performed in the video?\n1. Position one peristaltic feeder tube per tissue bath around rollers of peristaltic pump head\n2. Secure tubes with retaining stops by tightening compression cams\n3. Lock keys around tubes\n4. Place free ends of peristaltic feeder tubes into one liter container of physiological saline (PSS)\n5. Start pump to allow PSS to continually perfuse into tissue baths", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/56639/clip_1_removed_step_4.mp4"}} |
| {"qid": "ExpVid_7777", "question": "The study’s key advance is a standardized _____ localization of the _____ (M1) paired with precise _____ electrode montages (unilateral or bilateral), ensuring current reaches the target to reliably increase _____ and enhance motor skill learning.", "options": null, "answer": "TMS–MEP–guided | primary motor cortex | tDCS/tRNS | cortical excitability", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/53367_.mp4"}} |
| {"qid": "ExpVid_4555", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Crush 10 grams of commercial di-iodine pentoxide crystals to consistent powder using mortar and pestle\n2. Spread crushed powder in ceramic crucible\n3. Heat crucible at 10°C per minute to 250°C and hold for 5 minutes to remove iodic acid\n4. Place 3 grams of dried di-iodine pentoxide powder in glass beaker containing magnetic stirrer\n5. Add 3 grams of distilled water to beaker\n6. Stir mixture for 20 minutes to form aqueous iodate solution\n7. Heat solution in oven to 250°C at 20°C/minute rate\n8. Maintain solution at 250°C for 10 minutes\n9. Prepare aqueous iodate solution\n10. Stir solution in low humidity environment until water evaporates and iodic acid dehydrate precipitates\n11. Place solid iodic acid sample in differential scanning calorimeter\n12. Heat sample to 250°C at 10°C per minute\n13. Mix oxidizer with 80 nanometer aluminum in beaker to achieve 2 grams total weight\n14. Add 60 milliliters of isopropanol to beaker\n15. Sonicate mixture for 2 minutes\n16. Pour sonicated mixture into glass dish in fume hood with 20% relative humidity atmosphere\n17. Allow solvent to evaporate for 24 hours\n18. Ground razor blade with conductive wire\n19. Remove dry mixture from glass dish using grounded razor blade\n20. Sieve mixture into airtight container\n21. Sieving and mixing 80 nanometer aluminum and oxidizer to prepare a two-gram sample with fuel oxygen equivalence ratio of one\n22. Sealing the mixture in an airtight container\n23. Placing sealed container on vibration table for three minutes\n24. Heat alumina crucibles to 1500 degrees Celsius for 30 minutes to remove residues\n25. Weigh crucibles and record weights\n26. Place 10 mg of each mixture into a crucible\n27. Place sample and reference crucibles on DSC-TGA thermocouple\n28. Heat sample at 10°C/min to 600°C in argon atmosphere for thermal equilibrium analysis\n29. Cover one end of each quartz tube with electrical tape\n30. Weigh each tube\n31.\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "1, 2, 3", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/54661/clip_1.mp4"}} |
| {"qid": "ExpVid_4540", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Dissociate submerged BME2 organoid culture\n2. Aspirate media from culture plates while avoiding contact with culture\n3. Trypsinize submerged BME2 organoid culture with recombinant enzyme\n4. Add two milliliters of recombinant enzyme per well in six-well plate\n5. Mechanically break BME2 by repeated pipetting\n6. Incubate plates at 37°C in cell culture incubator for five minutes\n7. Transfer suspension to 15 milliliter centrifuge tube\n8. Centrifuge at 600 × g for 5 minutes at room temperature\n9. Aspirate recombinant enzyme supernatant carefully without disturbing organoid pellet\n10. Resuspend organoid pellet in growth medium\n11. Add DNase 1 to suspension\n12. Incubate suspension for 5 minutes at room temperature\n13. Centrifuge at 600 × g for 3 minutes at room temperature\n14. Discard media carefully without disturbing organoid pellet\n15. Resuspend pellet in fresh growth medium\n16. Preheat new black clear bottom 96-well plate at 37°C in cell culture incubator for 10 minutes\n17. Prepare cell suspension in PBS\n18. Count cells using cell analyzer\n19. Aliquot cells from single-cell suspension\n20. Pellet cells by centrifugation\n21. Discard media\n22. Keep cells on ice for approximately one minute\n23. Resuspend cells in BME2\n24. Tilt pre-warmed black clear bottom 96-well plate towards the body\n25. Seed five microliters of cell suspension per well\n26. Seed cell domes at six o'clock position of well\n27. Fill outer wells at rim of 96-well plate with PBS\n28. Seed cell domes in remaining inner wells\n29. Incubate freshly seeded cell domes in laminar flow hood for five minutes without moving plate\n30. Transfer plate to cell culture incubator\n31. Incubate plate at 37 degrees Celsius for 10 minutes\n32. Add 100 microliters of growth medium per well to wells containing organoids\n33. Add 100 microliters of PBS to outer wells at rim of plate\n34. Prepare serial drug dilution of osimertinib in low growth f\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "1, 2, 3, 4, 5, 6", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/63039/clip_1.mp4"}} |
| {"qid": "ExpVid_5223", "question": "What is the correct sequence of steps for macroscopic and histological validation of a decellularized intestinal scaffold?", "options": ["A. 1. Inject dye through SMA cannula\n2. Assess scaffold using DNA quantification kit\n3. Perform hematoxylin and eosin staining", "B. 1. Perform hematoxylin and eosin staining\n2. Inject dye through SMA cannula\n3. Assess scaffold using DNA quantification kit", "C. 1. Inject dye through SMA cannula\n2. Perform hematoxylin and eosin staining\n3. Assess scaffold using DNA quantification kit", "D. 1. Assess scaffold using DNA quantification kit\n2. Inject dye through SMA cannula\n3. Perform hematoxylin and eosin staining"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/50658/clip_11.mp4"}} |
| {"qid": "ExpVid_4100", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Crush dry ice block into fine flakes using hammer and hard flat object in 5L plastic container\n2. Sieve fine dry ice flakes from non-crushed material using large strainer into another 5L plastic container\n3. Pour 200 cubic centimeters of dry ice flakes into upright 1L glass beaker\n4. Add 10g frozen cannabis sativa inflorescences onto dry ice layer and cover with additional 200cc finely crushed dry ice\n5. Cover beaker opening with 2-3 layers of 1mm screen door mosquito net secured with rubber bands\n6. Pour liquid nitrogen into large round-bottom stainless steel container\n7. Insert 350 micrometer mesh in flour sifter to cover mesh from below\n8. Place flour sifter above stainless steel container filled with liquid nitrogen\n9. Shake one liter glass beaker with opening downward toward flour sifter to separate trichomes\n10. Set aside beaker every 2-3 minutes to allow sifting of accumulated dry ice and plant material on flour sieve\n11. Sift flour sieve horizontally to pass plant material into liquid nitrogen in stainless steel container below\n12. Add liquid nitrogen to stainless steel container when level runs low\n13. Add crushed dry ice to one liter glass beaker when level runs low\n14. Refill glass beaker containing used plant material with 10 grams of fresh plant material\n15. Repeat sifting process until sufficient enriched trichomes accumulate in container\n16. Verify presence of white powder-like substance at bottom of stainless steel container submerged in liquid nitrogen\n17. Add small amount of liquid nitrogen\n18. Add liquid nitrogen to clean small round-bottom stainless steel container\n19. Fold 40x40 cm microsieve (150 μm mesh) twice to obtain 20x20 cm fold\n20. Open folded microsieve into cone-like shape\n21. Secure mesh cone to container edge with clothespins, positioning cone upright with pointed end submerged in liquid nitrogen\n22. Pour liquid nitrogen containing white powder\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "24, 25, 26, 27, 28, 29", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/64798/clip_6.mp4"}} |
| {"qid": "ExpVid_6212", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Autoclave 15 grams of agar in 900 to 1000 milliliters of water\n2. Mix autoclaved agar with 166 grams of wheat germ diet in laboratory blender\n3. Pour blended insect diet into dish\n4. Allow diet to cool in dish\n5. Transfer cooled diet to aluminum foil, wrap tightly, and store at 4°C\n6. Place Manduca sexta eggs in glass filter holder with 90mm filter paper in vacuum flask apparatus\n7. Pour 250 milliliters of 0.6% bleach solution onto eggs for 2-3 minutes while stirring occasionally\n8. Turn on vacuum to drain bleach solution\n9. Wash sterilized eggs 3-4 times with 250 ml sterile distilled water per wash\n10. Place fresh 90 mm filter paper into Petri plate lid\n11. Transfer eggs to filter paper for drying in flow hood until no longer adhesive\n12. Place recently prepared insect diet on secured rubber stoppers in plastic containers\n13. Transfer approximately 40 eggs into each container\n14. Separate eggs from diet to prevent moisture-induced fungal contamination\n15. Maintain eggs at 26°C with 16-hour light/8-hour dark photoperiod\n16. Pick up each larva by the long black horn using forceps\n17. Transfer approximately 25 larvae to new containers with diet at the bottom\n18. Incubate larvae for two days under same conditions as eggs\n19. Transfer larvae to individual containers with small pieces of food\n20. Incubate larvae again\n21. Replace food and clean feces from containers every other day\n22. Centrifuge 500 microliters of bacterial strains at 17,000 G for 2 minutes at room temperature\n23. Wash pellets in 1 milliliter of sterile PBS under same centrifugation conditions\n24. Resuspend cells in 0.5 milliliter of sterile PBS\n25. Measure optical density of suspensions\n26. Prepare six serial tenfold dilutions of bacterial strain in sterile PBS using fresh pipette tips in 96-well microtiter plate\n27. Spot 10 microliters of d\nAnswer with the step number only.", "options": null, "answer": "21", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/4295/clip_4_prediction.mp4"}} |
| {"qid": "ExpVid_5942", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Split 2 milliliters of human plasma into two 1-milliliter aliquots: one for metabolite/protein corona and one for plasma metabolome characterization\n2. Incubate 1 milligram per milliliter of nanomaterials in human plasma for 1 hour at 37°C with gentle mixing at 500 rpm in thermo mixer\n3. Pellet nanomaterials by centrifugation at 4,000 × g for 15 minutes at 4°C\n4. Collect plasma supernatant\n5. Resuspend pellet in 1 mL of 10X PBS buffer\n6. Vortex vigorously for 2 minutes to remove unbound proteins\n7. Centrifuge solution at 4,000 × g for 15 minutes at 4°C\n8. Remove supernatant carefully without disturbing pellet\n9. Resuspend pellet in 1 mL of ABC buffer\n10. Resuspend pellet in 1 mL of ABC buffer\n11. Vortex mixture vigorously for 2 minutes\n12. Perform centrifugation\n13. Discard supernatant\n14. Dissolve pellet in 20 μL ABC buffer containing 10 mM dithiothreitol\n15. Incubate solution at 56°C for 30 minutes\n16. Add 2 micrograms of sequencing grade trypsin to 20 microliters of ABC buffer containing 0.1% surfactant\n17. Incubate digest solution at 37 degrees Celsius for 16 hours\n18. Add 20 microliters of 55 millimolar iodoacetamide in 100 millimolar ABC to alkylate sample\n19. Incubate at room temperature for 20 minutes\n20. Add 20 microliters of 0.1 molar hydrochloric acid to cleave surfactant\n21. Leave sample at room temperature for 10 minutes\n22. Take control plasma and supernatant from nanomaterial plasma incubation\n23. Place samples on ice\n24. Aliquot 50 microliters from each sample into separate vials\n25. Dilute samples tenfold with distilled water\n26. Transfer 50 microliters of diluted sample to new vials after vortexing\n27. Add 200 microliters of chloroform\n28. Add 250 microliters of methanol\n29. Add 350 microliters of distilled water\n30. Vortex mixture vigorously for two minutes\n31. Centrifuge at 20,800 × g \nAnswer with the step number only.", "options": null, "answer": "53", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/61760/clip_8_prediction.mp4"}} |
| {"qid": "ExpVid_4596", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Add 100 microliters of Proteinase K, 800 microliters of lysis buffer, and 1 microgram of carrier RNA to 1 milliliter of patient plasma\n2. Pulse vortex solution thoroughly for 30 seconds\n3. Incubate solution for 30 minutes at 60 degrees Celsius\n4. Add 1.8 milliliters of binding buffer to the tube\n5. Mix sample with 15 to 30 seconds of pulse vortexing\n6. Insert silica membrane column into vacuum apparatus\n7. Insert 20 milliliter tube extender into open column\n8. Pour mixture into tube extender\n9. Switch on vacuum pump\n10. Monitor lysate filtration until complete\n11. Switch off vacuum pump\n12. Release pressure to zero millibars\n13. Discard tube extender without contaminating columns\n14. Transfer column into collection tube\n15. Centrifuge column to remove residual lysate\n16. Discard flow-through and add 600 microliters of wash buffer 1 to column\n17. Centrifuge column with wash buffer 1\n18. Discard flow-through and add 750 microliters of wash buffer 2 to column\n19. Centrifuge column with wash buffer 2\n20. Discard flow-through and add 750 microliters of 96-100% ethanol to column\n21. Centrifuge column with ethanol\n22. Transfer column to new 2 ml collection tube\n23. Centrifuge column in collection tube\n24. Place membrane column assembly into new 2 ml collection tube\n25. Dry column at 56°C for 10 minutes\n26. Transfer column to new 1.5 ml elution tube\n27. Add 50 microliters of elution buffer to column\n28. Incubate at room temperature for 3 minutes\n29. Centrifuge solution at 20,000 × g for 1 minute to elute nucleic acids\n30. Secure base plate to chip priming station\n31. Adjust clip to lowest position\n32. Place high sensitivity DNA chip onto priming station\n33. Add 9 microliters of gel dye mix to G chip well\n34. Position plunger at 1 milliliter mark\n35. Close chip priming station\n36. Close lock of latch until it clicks\n37. Set timer to 60 seconds\n38. Press down plunger until held by clip\n\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "4, 5, 6, 7", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61449/clip_2.mp4"}} |
| {"qid": "ExpVid_5308", "question": "What is the correct sequence of steps for the methanol wash and debris processing experiment?", "options": ["A. 1. Add 20 milliliters of ice-cold methanol to two tubes containing debris\n2. Add 20 milliliters of ice-cold methanol to one tube containing protein interlayer\n3. Briefly vortex all tubes\n4. Centrifuge tubes at 4,000 × g and 4°C for 5 minutes\n5. Decant methanol supernatant into hazardous waste container inside fume hood", "B. 1. Add 20 milliliters of ice-cold methanol to two tubes containing debris\n2. Briefly vortex all tubes\n3. Add 20 milliliters of ice-cold methanol to one tube containing protein interlayer\n4. Centrifuge tubes at 4,000 × g and 4°C for 5 minutes\n5. Decant methanol supernatant into hazardous waste container inside fume hood", "C. 1. Add 20 milliliters of ice-cold methanol to two tubes containing debris\n2. Add 20 milliliters of ice-cold methanol to one tube containing protein interlayer\n3. Centrifuge tubes at 4,000 × g and 4°C for 5 minutes\n4. Briefly vortex all tubes\n5. Decant methanol supernatant into hazardous waste container inside fume hood", "D. 1. Add 20 milliliters of ice-cold methanol to one tube containing protein interlayer\n2. Add 20 milliliters of ice-cold methanol to two tubes containing debris\n3. Briefly vortex all tubes\n4. Centrifuge tubes at 4,000 × g and 4°C for 5 minutes\n5. Decant methanol supernatant into hazardous waste container inside fume hood"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57343/clip_7.mp4"}} |
| {"qid": "ExpVid_6904", "question": "Given the following step list，which step was not performed in the video?\n1. Excise one lung lobe from euthanized mouse\n2. Place excised lung lobe in 10×10×5 mm cryo mold\n3. Cover lung lobe with approximately 500 microliters of OCT compound\n4. Freeze sample on dry ice\n5. Maintain sample at freezing temperature\n6. Excise one decellularized lung lobe from processed mouse\n7. Place decellularized lobe in cryo mold with largest surface area down\n8. Cover decellularized lobe with OCT compound\n9. Freeze sample on dry ice\n10. Maintain sample at freezing temperature until required", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7", "H. 8", "I. 9", "J. 10"], "answer": "H", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/61854/clip_8_removed_step_8.mp4"}} |
| {"qid": "ExpVid_5808", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Use fertilized quail eggs (fresh or stored at 10-15°C for ≤5 days)\n2. Select only clean and undamaged eggs\n3. Incubate eggs in forced-draught incubator at 50-60% humidity, 37.5°C for ~54 hours (horizontal position, rotation off)\n4. Disinfect egg surface with 70% ethanol without rotating egg\n5. Open eggshell using sterile surgical scissors in laminar flow cabinet while wearing gloves\n6. Transfer egg content into 6-well culture plate\n7. Disinfect scissors with 70% ethanol after processing each egg\n8. Add approximately 5 milliliters of sterile water to gaps in 6-well plate to maintain humidity\n9. Aspirate improperly-tipped embryos or unfertilized eggs using vacuum aspirator\n10. Place embryos in incubator at 37°C with 80-90% humidity\n11. Place sterilized silicone ring on CAM surface along small capillaries while avoiding major blood vessels\n12. Apply 30 microliters of hypericin solution to silicone ring under sterile conditions\n13. Keep embryos in incubator at 37 degrees Celsius in 80-90% humidity\n14. Illuminate CAM with violet excitation light\n15. Record hypericin and CAM tissue fluorescence with digital camera at timed intervals\n16. Record CAM in white light before hypericin administration (if required)\n17. Record CAM in white light before tissue fixation (if required)\n18. Place CAM under optical fiber to cover entire silicone ring area with laser beam\n19. Perform in vivo irradiation with 405nm laser at 285 mW/cm² fluence rate\n20. Record CAM images using white light and/or fluorescent light before and after treatment\n21. Fix CAM tissue in cultivation plate with 4% paraformaldehyde in PBS\n22. Remove paraformaldehyde solution\n23. Cut out target tissue from CAM within silicone ring\n24. Wash excised CAM tissue in water for 10 minutes\n25. Dehydrate tissue in 70% ethanol for 3 minutes\n26. Stain tissue in eosin so\nAnswer with the step number only.", "options": null, "answer": "52", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/63422/clip_10_prediction.mp4"}} |
| {"qid": "ExpVid_5318", "question": "What is the correct sequence of steps for the negative control setup experimental procedure?", "options": ["A. 1. Remove plate from incubator\n2. Collect bacterial culture using sterile 1 microliter loop until loop is half full\n3. Invert negative control latex reagent several times\n4. Pipette 15 microliters of reagent onto labeled microscope slide\n5. Smear inoculum from loop onto slide near negative control reagent drop", "B. 1. Remove plate from incubator\n2. Invert negative control latex reagent several times\n3. Pipette 15 microliters of reagent onto labeled microscope slide\n4. Collect bacterial culture using sterile 1 microliter loop until loop is half full\n5. Smear inoculum from loop onto slide near negative control reagent drop", "C. 1. Remove plate from incubator\n2. Invert negative control latex reagent several times\n3. Collect bacterial culture using sterile 1 microliter loop until loop is half full\n4. Pipette 15 microliters of reagent onto labeled microscope slide\n5. Smear inoculum from loop onto slide near negative control reagent drop", "D. 1. Invert negative control latex reagent several times\n2. Remove plate from incubator\n3. Pipette 15 microliters of reagent onto labeled microscope slide\n4. Collect bacterial culture using sterile 1 microliter loop until loop is half full\n5. Smear inoculum from loop onto slide near negative control reagent drop"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/51747/clip_8.mp4"}} |
| {"qid": "ExpVid_6272", "question": "Given the following step list，which step was not performed in the video?\n1. Scan membrane and reference scale using stereomicroscope\n2. Calculate exposed membrane area from scanned image using graphical software", "options": ["A. 1", "B. 2"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/58301/clip_7_removed_step_1.mp4"}} |
| {"qid": "ExpVid_5802", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Prepare a 22-gauge syringe needle\n2. Blunt needle tip using honing stone\n3. Plicate needle at right angle using pliers\n4. Prepare required surgical materials including recovery cage\n5. Autoclave surgical instruments\n6. Place rat in supine position on surgery platform with heating pad\n7. Completely shave surgical area\n8. Scrub cleanly shaven abdomen with betadine or cleansing reagent\n9. Make 2 cm midline abdominal incision using scalpel\n10. Moisten abdominal organs with normal saline and maintain moisture during surgery\n11. Displace digestive organs to the side using cotton balls\n12. Expose inferior vena cava and posterior peritoneal region\n13. Identify abdominal aorta juxtaposed to left of inferior vena cava\n14. Pierce peritoneum with forceps to uncover vessels\n15. Gently isolate abdominal aorta adjacent to renal arteries\n16. Pass 8cm 4/0 silk suture underneath abdominal aorta between renal artery origins\n17. Make loose double knot with suture and leave 3 mm diameter loop\n18. Place blunted/bent 22-gauge needle inside suture loop\n19. Tighten suture knot around aorta and needle\n20. Remove needle to create 0.7 mm diameter aortic constriction\n21. Close abdominal cavity with 6/0 sutures\n22. Close abdominal cavity with 6/0 sutures\n23. Close muscle and skin incisions using simple interrupted sutures\n24. Scrub surgical site with iodine tincture\n25. Treat animal with acetaminophen for postsurgical pain\n26. Observe animal until regaining consciousness indicated by free movement, food intake, and sternal recumbency\n27. Weigh rat at 10 weeks postsurgery\n28. Anesthetize rat and confirm anesthesia depth\n29. Place anesthetized rat on metal tray\n30. Make 5 cm thoracic incision at midline of xiphoid process\n31. Pierce diaphragm using sharp forceps\n32. Cut and remove rib cage along midclavicular lines on both sides to expo\nAnswer with the step number only.", "options": null, "answer": "44", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/54818/clip_8_prediction.mp4"}} |
| {"qid": "ExpVid_7701", "question": "This study concludes that a ____ microglia culture protocol using ____ magnetic cell sorting yields highly pure cells within ____ in vitro and retains in vivo-like, stimulus-dependent phagocytosis, showing earlier bead uptake after ____ than after LPS.", "options": null, "answer": "serum-free | CD11b | 48 h | IL-1β + IFN-γ", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/62964_.mp4"}} |
| {"qid": "ExpVid_5658", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Add 5% sulfinitated polyetheretherketone fibers to 250 milliliter round bottom flask\n2. Dissolve fibers in dimethylacetamide solvent\n3. Shake flask for 10 minutes to settle ionomer polymers\n4. Place mixture into silicon oil bath with magnetic stir bar\n5. Vigorously stir solution at 500 rpm for 24 hours at 80°C\n6. Filter 30 milliliters of solution through 0.45 micrometer PTFE filter into circular 18 centimeter diameter glass dish\n7. Remove bubbles using air blower\n8. Place dish in oven at 90 degrees Celsius for 24 hours to generate approximately 50 micrometer thick freestanding membrane\n9. Fill dish with warm distilled water\n10. Wait 10 minutes for membrane detachment\n11. Immerse membrane in 1M sulfuric acid at 80°C for 2 hours\n12. Wash membrane three times with 1L distilled water (10 minutes per wash at room temperature)\n13. Dissolve 10% by weight FAA-3 ionomer in NMP solvent at room temperature with 500 RPM stirring for 2 hours\n14. Filter approximately 30 ml of solution through 100 micron pore strainer into 18 cm glass Petri dish\n15. Place dish in 100°C oven for 24 hours after air bubble removal\n16. Extract dried membrane using hot distilled water\n17. Activate membrane in 1 L sodium hydroxide solution for 2 hours\n18. Wash activated membrane three times with 1 L distilled water per wash\n19. Position 3 cm thick PMMA plate with electrode facing up\n20. Place rubber gasket and spacer onto electrode\n21. Place cation exchange membrane\n22. Place anion exchange membrane on opposite side of gasket\n23. Place silicon gasket and spacer onto each membrane\n24. Position second PMMA plate onto spacers and gaskets\n25. Secure stack using digital wrench drive at 25 newton meter force with nuts, bolts, and washers\n26. Place iridium/ruthenium-coated titanium mesh electrode at end of each plate\n27. Connect electrodes to source\nAnswer with the step number only.", "options": null, "answer": "18", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/62309/clip_5_prediction.mp4"}} |
| {"qid": "ExpVid_6611", "question": "Given the following step list，which step was not performed in the video?\n1. Position animal into light/dark place preference test box with inter-chamber passageway\n2. Configure apparatus with one brightly lit chamber and opposite dark chamber\n3. Monitor animal's light/dark occupancy times and transitions via computer during 10-minute test\n4. Place rodents on zero maze apparatus\n5. Assess time spent in closed/open maze areas using stopwatch", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/63997/clip_4_removed_step_3.mp4"}} |
| {"qid": "ExpVid_6815", "question": "Given the following step list，which step was not performed in the video?\n1. Aliquot 40 microliters of solution C to each well containing HEK293 cells\n2. Swirl plate gently\n3. Incubate plate at 37 degrees Celsius in humidified incubator with 5% carbon dioxide overnight\n4. Check NR1-GFP recombinant protein expression in host cells under fluorescent microscope at 40x to 200x magnification", "options": ["A. 1", "B. 2", "C. 3", "D. 4"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/56676/clip_3_removed_step_1.mp4"}} |
| {"qid": "ExpVid_5143", "question": "What is the correct sequence of steps for preparing the Gas Sampling Setup?", "options": ["A. 1. Measure distance from top of simulated bedded pack to top of plastic container\n2. Push metal stake approximately 5 cm into middle of bedded pack\n3. Thread 0.64 cm outer diameter tubing through hole at top of simulated bedded pack container\n4. Attach 12.5 cm metal stake to tubing inside simulator, positioning tubing 1.3 cm above bedded pack surface\n5. Place rubber skirts with 22.9 cm diameter holes onto static flux chambers\n6. Place static flux chambers with attached skirts onto bedded pack simulators", "B. 1. Measure distance from top of simulated bedded pack to top of plastic container\n2. Push metal stake approximately 5 cm into middle of bedded pack\n3. Place rubber skirts with 22.9 cm diameter holes onto static flux chambers\n4. Thread 0.64 cm outer diameter tubing through hole at top of simulated bedded pack container\n5. Attach 12.5 cm metal stake to tubing inside simulator, positioning tubing 1.3 cm above bedded pack surface\n6. Place static flux chambers with attached skirts onto bedded pack simulators", "C. 1. Measure distance from top of simulated bedded pack to top of plastic container\n2. Push metal stake approximately 5 cm into middle of bedded pack\n3. Attach 12.5 cm metal stake to tubing inside simulator, positioning tubing 1.3 cm above bedded pack surface\n4. Thread 0.64 cm outer diameter tubing through hole at top of simulated bedded pack container\n5. Place rubber skirts with 22.9 cm diameter holes onto static flux chambers\n6. Place static flux chambers with attached skirts onto bedded pack simulators", "D. 1. Push metal stake approximately 5 cm into middle of bedded pack\n2. Measure distance from top of simulated bedded pack to top of plastic container\n3. Thread 0.64 cm outer diameter tubing through hole at top of simulated bedded pack container\n4. Attach 12.5 cm metal stake to tubing inside simulator, positioning tubing 1.3 cm above bedded pack surface\n5. Place rubber skirts with 22.9 cm diameter holes onto static flux chambers\n6. Place static flux chambers with attached skirts onto bedded pack simulators"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57332/clip_3.mp4"}} |
| {"qid": "ExpVid_7311", "question": "In polysome profiling of E12 mouse cortex lysate separated into 12 fractions from eight embryos, _____ and _____ mRNAs were highly enriched in heavy polysome fractions, whereas _____ and _____ mRNAs were enriched in monosome fractions, reflecting efficient versus repressed translation.", "options": null, "answer": "Gapdh | sox2 | rpl7 | rpl35", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/62088_.mp4"}} |
| {"qid": "ExpVid_4465", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Dissect E14.5 mouse embryos from uterus\n2. Store embryos in L15 medium on ice\n3. Transfer embryo to microscope\n4. Isolate brain from embryo\n5. Remove cephalon by cutting along medial part of cephalic vesicles\n6. Remove meningeal sheath\n7. Make dorso-ventral cut rostral to mesencephalic flexure\n8. Make dorso-ventral cut caudal to mesencephalic flexure\n9. Make rostral-caudal cut along dorsal midline using microdissection knife\n10. Expose underlying ventral midbrain tissue\n11. Make first rostral-caudal cut lateral and parallel to ventral midline\n12. Make second rostral-caudal cut lateral and parallel to ventral midline\n13. Remove dorsal midbrain tissue\n14. Divide ventral midbrain tissue into explants using microdissection knife\n15. Store explants in L-15 medium containing 5% FBS on ice\n16. Transfer telencephalic vesicles to microscope\n17. Remove thalamus by cutting between thalamus and striatum using forceps\n18. Make medial-lateral cut rostral to striatum\n19. Remove rostral structures including olfactory bulb\n20. Repeat cutting procedure for tissue caudal to striatum\n21. Position remaining tissue slice for coronal view of striatum\n22. Identify striatum by its slightly more transparent tissue characteristic\n23. Isolate striatum tissue\n24. Cut striatum into explants using microdissection knife while avoiding darker tissue near midline containing ganglionic eminence neurons\n25. Add a drop of prepared collagen to a cover slip in a four-well dish\n26. Incubate at 37°C and 5% CO₂ for 30 minutes to gelatinize collagen\n27. Transfer dopaminergic or sal explants to gelatinized collagen using pipette with 200 microliter tip\n28. Position explants in close proximity using needle, maintaining distance of approximately one explant diameter\n29. Remove excess medium\n30. Add 20 microliters of prepared collagen on top of explant\n31. Reposition displaced explants using needle\n32. Solidify collagen at\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "29, 30, 31, 32, 33, 34", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/3691/clip_5.mp4"}} |
| {"qid": "ExpVid_6779", "question": "Given the following step list，which step was not performed in the video?\n1. Place liver in 100 mm Petri dish containing 5 ml fresh warm HBSS supplemented with Collagenase\n2. Use stray-tipped forceps to gently remove liver capsule\n3. Use forceps to carefully disperse parenchymal tissue\n4. Add 15 ml cold DMEM to Petri dish\n5. Shake torn liver gently to release residual parenchymal cells into medium\n6. Add another 15 ml cold DMEM to dish to acquire remaining cells", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6"], "answer": "E", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/58323/clip_3_removed_step_5.mp4"}} |
| {"qid": "ExpVid_6601", "question": "Given the following step list，which step was not performed in the video?\n1. Manually capture the boundary of the ceramic particle\n2. Cut the ceramic particle boundary region from the original image\n3. Set different threshold values to roughly determine the upper threshold of pore phases\n4. Segment the image and compare with the original image for threshold evaluation\n5. Obtain the gray-scale distribution of the remaining image region\n6. Choose two approximately linear parts of the curve above the predetermined upper threshold\n7. Fit the two linear curve parts with linear regression\n8. Set the intersection point of fitted curves as the exact upper threshold value\n9. Use the determined threshold value to perform image segmentation\n10. Compare the binary segmented image with the original gray-scale image for final threshold validation\n11. Convert the gray-scale image to a binary image with white representing pore phase and black representing solid phase", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7", "H. 8", "I. 9", "J. 10", "K. 11"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/60245/clip_8_removed_step_3.mp4"}} |
| {"qid": "ExpVid_6911", "question": "Given the following step list，which step was not performed in the video?\n1. Attach sterile 27-gauge needle to 1cc syringe on stereotaxic arm\n2. Position needle at 2.5mm lateral to BMA on skull surface\n3. Rotate syringe to initiate bur hole on skull surface\n4. Apply gentle downward force using frame\n5. Remove needle from stereotaxic arm after hole initiation\n6. Complete bur hole manually until inner table perforation sensation", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/2618/clip_3_removed_step_1.mp4"}} |
| {"qid": "ExpVid_5251", "question": "What is the correct sequence of steps for Subject Preparation and Electrode Placement?", "options": ["A. 1. Position subject in hospital gown sitting comfortably on wooden chair\n2. Place two ECG electrodes on right and left side of chest below collarbones respectively\n3. Measure subject's head circumference to select EEG net size\n4. Soak correct size EEG net for five minutes in solution of warm tap water, electrolytes, and baby shampoo\n5. Place five magnet coils (HPI) at known locations on scalp using micropore paper tape", "B. 1. Position subject in hospital gown sitting comfortably on wooden chair\n2. Measure subject's head circumference to select EEG net size\n3. Place two ECG electrodes on right and left side of chest below collarbones respectively\n4. Soak correct size EEG net for five minutes in solution of warm tap water, electrolytes, and baby shampoo\n5. Place five magnet coils (HPI) at known locations on scalp using micropore paper tape", "C. 1. Position subject in hospital gown sitting comfortably on wooden chair\n2. Measure subject's head circumference to select EEG net size\n3. Soak correct size EEG net for five minutes in solution of warm tap water, electrolytes, and baby shampoo\n4. Place five magnet coils (HPI) at known locations on scalp using micropore paper tape\n5. Place two ECG electrodes on right and left side of chest below collarbones respectively", "D. 1. Position subject in hospital gown sitting comfortably on wooden chair\n2. Measure subject's head circumference to select EEG net size\n3. Place five magnet coils (HPI) at known locations on scalp using micropore paper tape\n4. Soak correct size EEG net for five minutes in solution of warm tap water, electrolytes, and baby shampoo\n5. Place two ECG electrodes on right and left side of chest below collarbones respectively"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/201832/clip_1.mp4"}} |
| {"qid": "ExpVid_6563", "question": "Given the following step list，which step was not performed in the video?\n1. Dissolve 5 mg of conjugated polymers in 1 ml chloroform\n2. Dissolve 30 mg of SDS in 2 ml deionized water\n3. Add 100 μl of polymer mixture to SDS solution\n4. Homogenize mixture at 30,000 RPM for 2 minutes\n5. Allow emulsion to rest uncapped for 1 day to evaporate chloroform\n6. Centrifuge dispersion in 1.5 ml tube at 2,200 × g for 5 minutes\n7. Remove supernatant\n8. Add 1 ml deionized water and shake vigorously\n9. Repeat centrifugation, supernatant removal, and water wash three times", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7", "H. 8", "I. 9"], "answer": "E", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/55934/clip_2_removed_step_5.mp4"}} |
| {"qid": "ExpVid_4513", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Adjust CV-1 cell concentration to 100,000 cells per milliliter\n2. Seed 2 milliliters of cell suspension into each well of six-well plate\n3. Transfect cells with 0.5 ng gRNA-N2 plasmid and 0.5 ng pSTCas9 plasmid\n4. Incubate transfected cells for 24 hours\n5. Replace cell culture medium\n6. Dilute backbone VVL15 virus with DMEM to 200,000 PFU per milliliter\n7. Add 100 microliters of diluted VVL15 virus into each well of transfected CV-1 cells\n8. Transfect cells with 1 microgram of shuttle donor vector for HR expression\n9. Incubate transfected cells for 24 hours\n10. Detach transfected CV-1 cells using cell scraper\n11. Collect cell suspension into cryovial\n12. Store cryovial at -80°C for future use\n13. Seed 15 six-well plates with 300,000 unmodified CV-1 cells per well\n14. Thaw frozen suspension of transfected CV-1 cells at 37 degrees Celsius for 3 minutes\n15. Vigorously vortex cell suspension for 30 seconds to lyse cells\n16. Dilute 1 microliter of lysate with 3 milliliters of DMEM\n17. Add 0.5 milliliter of dilution to each plate well containing unmodified CV-1 cells\n18. Return plates to incubator for 2 days\n19. Identify RFP-positive plaques using fluorescence microscope with 10X objective\n20. Label identified plaques underneath plate using marker pen\n21. Prepare cryovial containing 200 microliters of serum-free DMEM for each positive plaque\n22. Aspirate all medium from wells with labeled plaques\n23. Set 200 microliter pipette to aspirate 30 microliters\n24. Partially load pipette with 10 microliters of medium from cryovial\n25. Scrape RFP-positive plaque and draw up cells with remaining aspiration\n26. Eject harvested material into cryovial\n27. Repeat scraping and ejection procedure twice more targeting same plaque\n28. Prepare six-well plate with 500,000 CV-1 cells per well\n29. Thaw frozen purified plaque at 37°C for 3 minutes\n30. Vortex plaque vigorously for 30 seconds to make lysate\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "28, 29, 30", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/54171/clip_7.mp4"}} |
| {"qid": "ExpVid_6171", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Prepare two clean 200-milliliter glass bottles\n2. Add 0.6 grams of sodium chloride to one bottle\n3. Add 0.5 grams of peptone to the bottle\n4. Add 3.4 grams of agar to the bottle\n5. Add 195 milliliters of distilled water to the bottle\n6. Add magnetic stirrer to the bottle containing ingredients\n7. Add magnetic stirrer to the other empty bottle\n8. Autoclave bottles for 15 minutes at 121 degrees Celsius\n9. Cool bottles in water bath for 30 minutes at 55 degrees Celsius\n10. Add 0.2 milliliters of 1M calcium chloride to filled bottle\n11. Add 0.2 milliliters of 5 mg/mL cholesterol to bottle\n12. Add 0.2 milliliters of 1M magnesium sulfate to bottle\n13. Add 5 milliliters of 1M potassium phosphate to bottle\n14. Mix solution by magnetic stirring on hot plate at 55°C\n15. Pour half of mixed NGM medium into empty 200-milliliter bottle\n16. Add one milliliter of DMSO to one bottle\n17. Mix DMSO-containing medium using magnetic stirring\n18. Store other bottle in water bath at 55 degrees Celsius\n19. Aliquot three milliliters of DMSO-containing medium to 35-by-10-millimeter Petri dishes\n20. Remove bottle from water bath\n21. Mix bottle contents using magnetic stirring\n22. Add 1 milliliter of 75-millimolar etoposide\n23. Repeat aliquoting procedure after mixing\n24. Cool chemical-containing NGM plates at room temperature for approximately three hours and store at 4°C\n25. Spread 100 microliters of heat-inactivated E. coli OP50 on each NGM plate\n26. Allow plates to dry overnight in C. elegans incubator at 20 degrees Celsius\n27. Transfer age-synchronized C. elegans eggs to NGM plates supplemented with 1% DMSO or 750-micromolar etoposide\n28. Place C. elegans plates in incubator at 20 degrees Celsius for four days\n29. Observe and take daily microscopic images of C. elegans under stereo microscope\n30. Take microscopic image of micros\nAnswer with the step number only.", "options": null, "answer": "39", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/56437/clip_7_prediction.mp4"}} |
| {"qid": "ExpVid_7731", "question": "According to the study, the key advance is a ____ implantation protocol that establishes ____ renal tumors in mice that metastasize to the lungs; tumor burden and metastasis were validated by ____ imaging of luciferase-expressing Renca cells, Cytokeratin 8/18 immunohistochemistry, and lung nodule quantification after India Ink inflation with fixation in ____.", "options": null, "answer": "intrarenal | orthotopic | bioluminescent | Fekete's solution", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/55080_.mp4"}} |
| {"qid": "ExpVid_4401", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Add 25 mm magnetic stir bar and 113 ml deionized water to 500 ml beaker\n2. Stir deionized water at highest rate without vortex formation\n3. Slowly add 13.05 g anhydrous Zirconium Chloride to water in small increments\n4. Add 53.29 g glucose to solution\n5. Add 3.73 g Yttrium nitrate hexahydrate to solution\n6. Increase stirring rate to 700 RPM until all solids dissolve\n7. Add 42 ml propylene oxide to solution\n8. Decrease stirring to 150 RPM after sufficient mixing\n9. Continue stirring until magnetic stir bar stops due to gel formation\n10. Remove parafilm from beaker\n11. Decant liquid on top of gel\n12. Add 300 milliliters of absolute ethanol to beaker containing gel\n13. Tightly cover beaker with parafilm\n14. Leave covered beaker at room temperature for 24 hours\n15. Remove gel from beaker\n16. Transfer gel to two-liter porcelain evaporating dish using laboratory spatula\n17. Break gel into pieces with spatula\n18. Spread gel pieces over surface of evaporating dish\n19. Allow gel pieces to dry under ambient conditions\n20. Grind dried xerogel into fine powder using agate mortar and pestle\n21. Place one gram of xerogel powder into 13mm cylindrical pellet press die\n22. Apply 22 kilo Newtons of force using hydraulic press for 90 seconds to form pellet\n23. Slowly release force from hydraulic press\n24. Carefully remove pellet from pellet die\n25. Place xerogel pellet onto YSZ plate and load into center of tube furnace\n26. Blow Argon at 1/3 working tube volume per minute for ≥15 minutes while venting outlet to fume hood\n27. Program tube furnace temperature controller to heating schedule while maintaining constant Argon flow\n28. Start heating program and verify furnace follows schedule\n29. Remove pellet from tube furnace after program completion\n30. Cut 50 milligram piece from centered xerogel pellet using utility knife\n31. Grind xerogel into fine powder using agate mortar and pestle\n32. Place a\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/55500/clip_2.mp4"}} |
| {"qid": "ExpVid_5266", "question": "What is the correct sequence of steps for lipid film preparation and emulsification?", "options": ["A. 1. Add 20 microliters of freshly prepared POPC solution and 4 microliters of freshly prepared biotin-PEG-DSPE solution to a glass vial\n2. Evaporate organic solvent by air flow until lipid film forms\n3. Place lipid film in desiccator for one hour to completely evaporate organic solvent\n4. Add 200 microliters of mineral oil to the vial\n5. Cover vial opening with plastic paraffin film\n6. Sonicate vial contents in ultrasonic bath at 120 watts for at least one hour", "B. 1. Add 20 microliters of freshly prepared POPC solution and 4 microliters of freshly prepared biotin-PEG-DSPE solution to a glass vial\n2. Evaporate organic solvent by air flow until lipid film forms\n3. Place lipid film in desiccator for one hour to completely evaporate organic solvent\n4. Cover vial opening with plastic paraffin film\n5. Add 200 microliters of mineral oil to the vial\n6. Sonicate vial contents in ultrasonic bath at 120 watts for at least one hour", "C. 1. Sonicate vial contents in ultrasonic bath at 120 watts for at least one hour\n2. Add 20 microliters of freshly prepared POPC solution and 4 microliters of freshly prepared biotin-PEG-DSPE solution to a glass vial\n3. Evaporate organic solvent by air flow until lipid film forms\n4. Place lipid film in desiccator for one hour to completely evaporate organic solvent\n5. Add 200 microliters of mineral oil to the vial\n6. Cover vial opening with plastic paraffin film", "D. 1. Add 20 microliters of freshly prepared POPC solution and 4 microliters of freshly prepared biotin-PEG-DSPE solution to a glass vial\n2. Evaporate organic solvent by air flow until lipid film forms\n3. Add 200 microliters of mineral oil to the vial\n4. Place lipid film in desiccator for one hour to completely evaporate organic solvent\n5. Cover vial opening with plastic paraffin film\n6. Sonicate vial contents in ultrasonic bath at 120 watts for at least one hour"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/59555/clip_2.mp4"}} |
| {"qid": "ExpVid_5497", "question": "What is the correct sequence of steps for setting up mouse embryo dissection and head fixation?", "options": ["A. 1. Transfer one embryo under dissection scope to six cm plate containing ice-cold HBSS\n2. Fix embryo head position using 21-23 gauge needles by penetrating through eyes at 45° angle and applying force to secure needles to plate\n3. Collect CD1 mouse embryos at embryonic day 12 and place them in 10 cm plate containing ice-cold HBSS on ice", "B. 1. Collect CD1 mouse embryos at embryonic day 12 and place them in 10 cm plate containing ice-cold HBSS on ice\n2. Fix embryo head position using 21-23 gauge needles by penetrating through eyes at 45° angle and applying force to secure needles to plate\n3. Transfer one embryo under dissection scope to six cm plate containing ice-cold HBSS", "C. 1. Collect CD1 mouse embryos at embryonic day 12 and place them in 10 cm plate containing ice-cold HBSS on ice\n2. Transfer one embryo under dissection scope to six cm plate containing ice-cold HBSS\n3. Fix embryo head position using 21-23 gauge needles by penetrating through eyes at 45° angle and applying force to secure needles to plate", "D. 1. Transfer one embryo under dissection scope to six cm plate containing ice-cold HBSS\n2. Collect CD1 mouse embryos at embryonic day 12 and place them in 10 cm plate containing ice-cold HBSS on ice\n3. Fix embryo head position using 21-23 gauge needles by penetrating through eyes at 45° angle and applying force to secure needles to plate"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/62088/clip_2.mp4"}} |
| {"qid": "ExpVid_7416", "question": "The methodological innovation shown is that maintaining ________ perfusion pressures (about ________) during transcardial perfusion fixation exploits the ________ to distribute fixative uniformly, enabling rapid, high-quality ________ preservation for immunohistochemistry.", "options": null, "answer": "physiological | 80-130 mmHg | vascular system | rat brain", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/3564_.mp4"}} |
| {"qid": "ExpVid_6640", "question": "Given the following step list，which step was not performed in the video?\n1. Load tissue lysate samples onto sucrose gradients by slowly dispensing to ultracentrifuge tube walls\n2. Gently place sucrose gradients in ultracentrifuge buckets\n3. Ultracentrifuge samples at 190,000 x g and 4°C for 90 minutes\n4. Place empty ultracentrifuge tube on tube piercer and gently penetrate tube", "options": ["A. 1", "B. 2", "C. 3", "D. 4"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/62088/clip_6_removed_step_1.mp4"}} |
| {"qid": "ExpVid_6192", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Prepare NMP binder mixture of 8% PVDF in NMP\n2. Mix active material and carbon black using vortex mixer for 1 minute\n3. Add NMP binder mixture to ingredients and transfer to small glass vial\n4. Vortex sample at maximum RPM for 30 minutes\n5. Add two 5mm diameter zirconia balls if needed for better mixing\n6. Add NMP as needed to achieve required slurry consistency\n7. Spread metal foil of current collector onto glass plate\n8. Use acetone to remove air bubbles between foil and glass plate\n9. Position two layers of masking tape to define coating region\n10. Apply slurry onto metal foil with stainless steel spatula\n11. Spread slurry uniformly using razor blade\n12. Dry coating in air or vacuum at 90-120°C for 2-8 hours\n13. Place dried coated metal foil between two steel plates lined with weighing papers\n14. Press assembly under approximately 3000-pound load\n15. Punch 8mm discs from dried coated metal foil\n16. Weigh punched cathode discs\n17. Wrap cathodes and transfer to glove box\n18. Punch 8mm discs from uncoated metal foil\n19. Weigh uncoated foil discs\n20. Store electrolyte in Nalgene bottle wrapped in aluminum foil\n21. Place stored electrolyte bottle inside Argon glove box\n22. Clean lithium foil surface using nylon brush or stainless steel scalpel until shiny silvery surface appears\n23. Punch 12.7 millimeter discs from lithium foil\n24. Prepare 19 millimeter discs of Cellgard C480 membrane for separators\n25. Flush exchanger five times with argon\n26. Transfer working electrode, coin cell cases, springs, spacers, and separators onto glove box working surface\n27. Add two drops of electrolyte onto cell cup\n28. Position working electrode in cell cup\n29. Add three drops of electrolyte onto assembly\n30. Place two separators with two drops of electrolyte between them\n31. Add two drops of electrolyte onto separators\n32. \nAnswer with the step number only.", "options": null, "answer": "19", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/4104/clip_3_prediction.mp4"}} |
| {"qid": "ExpVid_4232", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Label a 15 milliliter conical tube for tissue sample\n2. Add 2.5 milliliters of type four collagenase mixture and 30 units of elastase to the tube\n3. Move lung to clean 1X HBSS well\n4. Swirl lung in HBSS with forceps to remove remaining blood\n5. Transfer lung to empty 3.5 centimeter tissue culture plate\n6. Mince lung with scissors\n7. Rinse plate with 2.5 milliliters of HBSS\n8. Transfer HBSS with lung pieces to prepared 15 milliliter conical tube containing collagenase/elastase cocktail\n9. Incubate tube for 75 minutes at 4°C on rocker/rotating wheel\n10. Label one 50 ml centrifuge tube and one 10 cm tissue culture plate per mouse (including additional plates for dilutions if required)\n11. Bring tube volume to 10 ml with 1X HBSS, pour contents over 70 μm cell strainer into 50 ml conical tube\n12. Place 70 micrometer cell strainer into 50 milliliter conical tube\n13. Use plunger of 1 milliliter syringe to gently grind sample through strainer\n14. Centrifuge tube for 5 minutes at 350 × g\n15. Discard supernatant after centrifugation\n16. Wash pellet twice with 10 milliliters of 1X HBSS\n17. Resuspend pellet in 10 milliliters of 60 micromolar 6-thioguanine complete culture media\n18. Plate samples in 10 centimeter cell culture plates\n19. Incubate plates at 37°C and 5% CO₂ for 5 days\n20. Pour culture media off plates into appropriate waste container\n21. Add 5 ml undiluted methanol to each plate\n22. Incubate plates with methanol for 5 minutes at room temperature\n23. Swirl methanol to ensure full plate coverage during incubation\n24. Pour methanol off plates into appropriate waste container\n25. Rinse each plate with 5 ml distilled water\n26. Add 5 ml of 0.03% methylene blue per plate\n27. Incubate plates with methylene blue for 5 minutes at room temperature\n28. Swirl methylene blue solution to ensure full plate coverage during incubation\n29. Pour methylene blue into appropriate waste container\n30\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "29, 30, 31, 32, 33", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61805/clip_5.mp4"}} |
| {"qid": "ExpVid_6225", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Dissociate submerged BME2 organoid culture\n2. Aspirate media from culture plates while avoiding contact with culture\n3. Trypsinize submerged BME2 organoid culture with recombinant enzyme\n4. Add two milliliters of recombinant enzyme per well in six-well plate\n5. Mechanically break BME2 by repeated pipetting\n6. Incubate plates at 37°C in cell culture incubator for five minutes\n7. Transfer suspension to 15 milliliter centrifuge tube\n8. Centrifuge at 600 × g for 5 minutes at room temperature\n9. Aspirate recombinant enzyme supernatant carefully without disturbing organoid pellet\n10. Resuspend organoid pellet in growth medium\n11. Add DNase 1 to suspension\n12. Incubate suspension for 5 minutes at room temperature\n13. Centrifuge at 600 × g for 3 minutes at room temperature\n14. Discard media carefully without disturbing organoid pellet\n15. Resuspend pellet in fresh growth medium\n16. Preheat new black clear bottom 96-well plate at 37°C in cell culture incubator for 10 minutes\n17. Prepare cell suspension in PBS\n18. Count cells using cell analyzer\n19. Aliquot cells from single-cell suspension\n20. Pellet cells by centrifugation\n21. Discard media\n22. Keep cells on ice for approximately one minute\n23. Resuspend cells in BME2\n24. Tilt pre-warmed black clear bottom 96-well plate towards the body\n25. Seed five microliters of cell suspension per well\n26. Seed cell domes at six o'clock position of well\n27. Fill outer wells at rim of 96-well plate with PBS\n28. Seed cell domes in remaining inner wells\n29. Incubate freshly seeded cell domes in laminar flow hood for five minutes without moving plate\n30. Transfer plate to cell culture incubator\n31. Incubate plate at 37 degrees Celsius for 10 minutes\n32. Add 100 microliters of growth medium per well to wells containing organoids\n33. Add 100 microliters of PBS to outer wells at rim\nAnswer with the step number only.", "options": null, "answer": "6", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/63039/clip_1_prediction.mp4"}} |
| {"qid": "ExpVid_7002", "question": "Given the following step list，which step was not performed in the video?\n1. Mix sample by pipetting\n2. Lyse cells by heating to 95 degrees Celsius for 2 minutes using benchtop thermocycler or boiling waterbath\n3. Perform PCR using 1 microliter of lysed cells as DNA template under specified conditions\n4. Inoculate 5 ml culture with verified sRNA clone\n5. Grow inoculated culture overnight\n6. Combine 750 microliters of overnight culture with 250 microliters of 60% glycerol in screwcap cryotube", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/53618/clip_4_removed_step_3.mp4"}} |
| {"qid": "ExpVid_6622", "question": "Given the following step list，which step was not performed in the video?\n1. Transfer the unfolded hippocampus to the recording chamber using a custom made glass pipette dropper\n2. Position the unfolded hippocampus under microscope with alveus side facing down, CA3 area pointing away, and CA1 field pointing towards researcher\n3. Aspirate solution from recording chamber edge using vacuum pipette until chamber is dried and tissue lies on array\n4. Place custom tissue anchor on top of tissue to secure unfolded hippocampus onto array", "options": ["A. 1", "B. 2", "C. 3", "D. 4"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/52601/clip_8_removed_step_3.mp4"}} |
| {"qid": "ExpVid_7667", "question": "The study’s central advance was a prospective, ______ that incorporated a ______, operationalizing metacognitive responding in rats by requiring a ______ and predicting higher accuracy on chosen than ______ trials.", "options": null, "answer": "odor-based delayed match-to-sample (DMTS) paradigm | decline-test option | metamemory choice | forced", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/57489_.mp4"}} |
| {"qid": "ExpVid_6134", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Mount clean 2 cm × 2 cm C-plane sapphire substrate on RF sputtering system sample stage with polished side facing molybdenum target\n2. Pump down sample chamber to 3 × 10⁻⁶ Torr\n3. Inject argon gas into system at 40 mL/min\n4. Stabilize chamber pressure at 5 × 10⁻² Torr\n5. Ignite plasma and set power output to 40 W\n6. Reduce chamber pressure to 5 × 10⁻³ Torr\n7. Manually open molybdenum target shutter\n8. Deposit metal for 30 seconds while maintaining constant power output\n9. Place substrate in quartz sample holder with metal film facing up\n10. Position substrate at center of heating zone in calibrated tube furnace\n11. Measure and place 1.5 grams of sulfur powder into alumina heating boat\n12. Position sulfur boat 2 cm upstream of heating zone to achieve 120°C at 800°C substrate temperature\n13. Close furnace and pump down to 5 × 10⁻³ torr\n14. Flow argon gas through furnace at 130 mL/min\n15. Stabilize furnace pressure at 0.7 torr\n16. Ramp furnace temperature from room temperature to 800°C at 20°C/min\n17. Hold furnace at 800°C until sulfur fully evaporates\n18. Turn off furnace heat and cool substrate to room temperature under argon flow\n19. Mount substrate in RF sputtering system with molybdenum disulfide film facing tungsten target\n20. Sputter tungsten on substrate for 30 seconds using same settings as for molybdenum\n21. Place tungsten-coated substrate in center of furnace heating zone\n22. Place one gram of sulfur powder two centimeters upstream of heating zone\n23. Sulfurize tungsten film using same parameters as for molybdenum film\n24. Spin coat three drops of polymethyl methacrylate on prepared transition metal dichalcogenide film\n25. Perform spin coating at 500 and 800 rotations per minute for 10 seconds each\n26. Cure PMMA at 120 degrees Celsius for five minutes\n27. Place PMMA-coated substrate in Petri dish \nAnswer with the step number only.", "options": null, "answer": "23", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/56494/clip_4_prediction.mp4"}} |
| {"qid": "ExpVid_4650", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Sterilize scalpel with 70% ethanol-moistened paper towels\n2. Place cover slip in 35 millimeter cell culture dish using scalpel\n3. Add one milliliter of culture medium to dish with cover slip\n4. Hold cover slip center with sterile forceps\n5. Aspirate culture medium completely from dish\n6. Prepare three milliliter cell suspension in culture medium in 15 milliliter tube\n7. Add cell suspension to culture dish\n8. Incubate sample overnight at 37°C and 5% CO₂\n9. Verify cell attachment and viability using inverted phase-contrast microscope\n10. Irradiate dish with 6 Gy X-ray radiation\n11. Incubate cells for 72 hours at 37°C and 5% CO₂\n12. Take cells from incubator\n13. Aspirate culture medium from culture dish\n14. Cut 5 mm from end of 1,000 microliter micropipette tip using scissors\n15. Add 1 milliliter fixation solution to culture dish along walls to minimize cell damage\n16. Transfer culture dish into square culture dish and shake gently to distribute fixation solution evenly over cover slip\n17. Incubate dish for 10 minutes at room temperature\n18. Aspirate fixation solution from dish\n19. Add 2 milliliters of PBS along dish walls\n20. Aspirate PBS from dish\n21. Add 5 microliters of DAPI staining reagent onto glass slide\n22. Remove cover slip from dish using scalpel\n23. Drain excess PBS by touching edge of cover slip with paper towel\n24. Mount cover slip upside down on DAPI staining reagent drop on glass slide\n25. Examine stained cells under fluorescence microscope with DAPI filter\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "8, 9, 10, 11", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/56338/clip_2.mp4"}} |
| {"qid": "ExpVid_5534", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Seed BT-474 cancer cells at density of 2 × 10⁶ cells into T-75 flask using 10 ml complete growth media\n2. Culture cells until reaching 70-80% confluency\n3. Discard growth media\n4. Wash cell monolayer with PBS\n5. Wash cell monolayer with PBS (second wash)\n6. Add 5 ml pre-warmed cell culture dissociation reagent\n7. Incubate at 37°C for 5 minutes or until cell detachment\n8. Remove flask from incubator\n9. Gently tap flask to aid cell detachment\n10. Add 5 ml complete growth media with 10% FBS to quench dissociation reagent\n11. Gently resuspend cells by pipetting to reduce clumps\n12. Transfer cell suspension to 50-milliliter tube\n13. Centrifuge at 180 G for 3 minutes at room temperature\n14. Decant supernatant\n15. Resuspend cell pellet in 10 ml HBSS without calcium and magnesium\n16. Centrifuge cell suspension at 180 G for 3 minutes at room temperature\n17. Decant supernatant to remove residual serum or dissociation reagent\n18. Resuspend cell pellet in 3 ml HBSS\n19. Place 100-micrometer cell strainer on fresh 50-milliliter conical tube\n20. Pass cell suspension through strainer to remove cell clumps\n21. Calculate number of viable cells using trypan blue\n22. Dilute cell suspension with HBSS to concentration of 2.5 × 10⁶ cells/mL\n23. Place tube horizontally on ice\n24. Gently rock tube periodically to minimize clumping\n25. Store cell suspension on ice\n26. Ear-punch mouse for identification\n27. Shave fur from neck region using electric clippers\n28. Clean excess hair from exposed skin using tape\n29. Apply ocular lubricant to eyes to prevent drying\n30. Secure mouse by hooking upper incisor teeth using thread taped to surgical board\n31. Tape front and hind legs to surgical board\n32. Wipe neck with povidone iodine antiseptic (center to outward)\n33. Perform three alternating rounds of povidone iodine and 70% ethanol disinfe\nAnswer with the step number only.", "options": null, "answer": "61", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/64216/clip_8_prediction.mp4"}} |
| {"qid": "ExpVid_6174", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Culture transduced human breast cancer MDA-MB-231 cells, breast epithelial MCF10A cells, and breast epithelial MCF10A-Ras cells in T75 flask\n2. Monitor cells until reaching 80% confluence\n3. Remove medium from T75 flask\n4. Wash cells with phosphate-buffered saline\n5. Detach cells using 0.5% Trypsin-EDTA solution\n6. Neutralize Trypsin with medium\n7. Centrifuge cells at 1,000 RPM for 5 minutes to remove medium\n8. Wash cells with PBS\n9. Centrifuge sample again\n10. Resuspend cells in approximately 200 microliters of PBS to achieve concentration ≤1×10⁸ cells/mL\n11. Store cell suspension at 4°C for ≤5 hours\n12. Incubate approximately 60 zebrafish embryos in a petri dish filled with egg water supplemented with 60 micrograms per milliliter of sea salts at 28 degrees Celsius\n13. Remove the coriands from embryos at 48 hours post-fertilization using fine tweezers\n14. Transfer embryos to a solution of 40 micrograms per milliliter tricaine in egg water for anesthesia\n15. Load borosilicate microcapillary tube into micro-pipette puller\n16. Pull needle using micro-pipette puller\n17. Store prepared needles in needle-holder plate\n18. Aspirate 15 microliters of prepared cell suspension into injection needle\n19. Attach needle to micromanipulator\n20. Break needle tip to 5-10 microns using fine tweezers\n21. Adjust pneumatic PICO pump\n22. Inject cells onto petri dish pre-coated with sterile 1% agarose\n23. Transfer approximately 10 anesthetized embryos at two to three days post-fertilization onto flat injecting plate coated with 1% agarose\n24. Arrange embryo positions with hair loop tool to orient embryos in same direction\n25. Manually adjust injection plate position to place embryos in diagonal orientation for precise needle insertion\n26. Direct needle tip at injection site and gently insert into perivitelline space between yol\nAnswer with the step number only.", "options": null, "answer": "27", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/55459/clip_5_prediction.mp4"}} |
| {"qid": "ExpVid_4836", "question": "What is the correct sequence of steps for the lignin drying and ash correction experiment?", "options": ["A. 1. Transfer solid to Petri dish\n2. Dry lignin and ash in oven at 60°C for 16 hours\n3. Further dry sample in oven at 105°C for 1 hour\n4. Place dried sample in desiccator to cool\n5. Weigh cooled sample\n6. Heat sample at 650°C for 5 hours in air for ash correction", "B. 1. Dry lignin and ash in oven at 60°C for 16 hours\n2. Transfer solid to Petri dish\n3. Place dried sample in desiccator to cool\n4. Weigh cooled sample\n5. Further dry sample in oven at 105°C for 1 hour\n6. Heat sample at 650°C for 5 hours in air for ash correction", "C. 1. Dry lignin and ash in oven at 60°C for 16 hours\n2. Further dry sample in oven at 105°C for 1 hour\n3. Transfer solid to Petri dish\n4. Place dried sample in desiccator to cool\n5. Weigh cooled sample\n6. Heat sample at 650°C for 5 hours in air for ash correction", "D. 1. Dry lignin and ash in oven at 60°C for 16 hours\n2. Transfer solid to Petri dish\n3. Further dry sample in oven at 105°C for 1 hour\n4. Place dried sample in desiccator to cool\n5. Weigh cooled sample\n6. Heat sample at 650°C for 5 hours in air for ash correction"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57613/clip_5.mp4"}} |
| {"qid": "ExpVid_5867", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Pour molten agarose into reagent trough\n2. Dispense 80 microliters of molten agarose into each well of 96-well microplate using multi-channel pipette\n3. Refrigerate leftover agarose in sealed bag for up to one week\n4. Coil barrier strips around finger to add curvature for secure channel placement\n5. Insert barrier strips into barrier strip channels\n6. Affix coupler to starvation plate without using coupler to manipulate plate\n7. Align angled corner of coupler with angled corner of microplate for correct orientation\n8. Sort three- to five-day-old flies under CO₂ anesthesia\n9. Load individual flies by column into starvation plate\n10. Close barrier strip per column as filled\n11. Record sample layout within microplate\n12. Remove CO₂ from flies\n13. Allow flies to recover spontaneously\n14. Initiate six-hour starvation period from anesthesia time\n15. Dissolve 0.4 grams of sucrose and 0.1 gram of yeast extract in 10 milliliters of distilled water in a 15 milliliter conical tube\n16. Vortex the tube until solids fully dissolve\n17. Add 40 microliters of dye stock solution to the liquid food\n18. Transfer liquid food into a 10 milliliter syringe tipped with 0.45 micrometer filter\n19. Filter approximately 1.5 milliliters of solution into a 1.7 milliliter microcentrifuge tube\n20. Set syringe containing solution aside for later filtration during feeder plate preparation\n21. Seal bottom of 1536-well microplate with sealing film\n22. Adhere film thoroughly using sealing paddle\n23. Trim excess film from left and right edges with razor blade\n24. Dispense 10 microliters of filtered liquid food column-wise into upper left-hand well of each 4-well cluster\n25. Apply sealing film to top of plate\n26. Adhere top film using same sealing steps as bottom\n27. Centrifuge plates at 200×g for 10 seconds to settle fluid\n28. Perforate wells \nAnswer with the step number only.", "options": null, "answer": "20", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/62771/clip_4_prediction.mp4"}} |
| {"qid": "ExpVid_5225", "question": "What is the correct sequence of steps for cryosectioning and H&E staining?", "options": ["A. 1. Section frozen tissue blocks into 5 micrometer sections using cryostat at -20°C\n2. Place tissue sections on adhesive glass slides\n3. Transfer slides to room temperature until air-dried\n4. Immerse slides in 4% paraformaldehyde in PBS for 15 minutes\n5. Briefly immerse slides in PBS\n6. Wash slides twice in distilled water (5 minutes per wash)\n7. Wash slides once in PBS\n8. Immerse slides in Myer's hematoxylin solution for 10 minutes\n9. Wash slides under running distilled water in Copeland jar for 10 minutes\n10. Immerse slides in eosin solution for 7 minutes\n11. Dip slides in xylene several times\n12. Apply few drops of DPX mounting medium to slides\n13. Place glass cover slip on slides\n14. Leave slides to dry overnight under chemical hood\n15. Scan dried slides in slide scanner", "B. 1. Section frozen tissue blocks into 5 micrometer sections using cryostat at -20°C\n2. Place tissue sections on adhesive glass slides\n3. Briefly immerse slides in PBS\n4. Transfer slides to room temperature until air-dried\n5. Immerse slides in 4% paraformaldehyde in PBS for 15 minutes\n6. Wash slides once in PBS\n7. Wash slides twice in distilled water (5 minutes per wash)\n8. Immerse slides in Myer's hematoxylin solution for 10 minutes\n9. Wash slides under running distilled water in Copeland jar for 10 minutes\n10. Immerse slides in eosin solution for 7 minutes\n11. Dip slides in xylene several times\n12. Apply few drops of DPX mounting medium to slides\n13. Place glass cover slip on slides\n14. Leave slides to dry overnight under chemical hood\n15. Scan dried slides in slide scanner", "C. 1. Section frozen tissue blocks into 5 micrometer sections using cryostat at -20°C\n2. Place tissue sections on adhesive glass slides\n3. Transfer slides to room temperature until air-dried\n4. Briefly immerse slides in PBS\n5. Immerse slides in 4% paraformaldehyde in PBS for 15 minutes\n6. Wash slides once in PBS\n7. Wash slides twice in distilled water (5 minutes per wash)\n8. Immerse slides in Myer's hematoxylin solution for 10 minutes\n9. Wash slides under running distilled water in Copeland jar for 10 minutes\n10. Immerse slides in eosin solution for 7 minutes\n11. Dip slides in xylene several times\n12. Apply few drops of DPX mounting medium to slides\n13. Place glass cover slip on slides\n14. Leave slides to dry overnight under chemical hood\n15. Scan dried slides in slide scanner", "D. 1. Section frozen tissue blocks into 5 micrometer sections using cryostat at -20°C\n2. Place tissue sections on adhesive glass slides\n3. Transfer slides to room temperature until air-dried\n4. Dip slides in xylene several times\n5. Immerse slides in 4% paraformaldehyde in PBS for 15 minutes\n6. Briefly immerse slides in PBS\n7. Wash slides once in PBS\n8. Wash slides twice in distilled water (5 minutes per wash)\n9. Immerse slides in eosin solution for 7 minutes\n10. Immerse slides in Myer's hematoxylin solution for 10 minutes\n11. Wash slides under running distilled water in Copeland jar for 10 minutes\n12. Place glass cover slip on slides\n13. Apply few drops of DPX mounting medium to slides\n14. Leave slides to dry overnight under chemical hood\n15. Scan dried slides in slide scanner"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/61854/clip_9.mp4"}} |
| {"qid": "ExpVid_6726", "question": "Given the following step list，which step was not performed in the video?\n1. Pre-incubate slide samples with 20% normal serum from secondary antibody host species in Tris-buffered saline for 30 minutes\n2. Apply 200 microliters of primary antibody solution directly into each well of slide-holder set\n3. Incubate primary antibody-treated slides for 60 minutes at room temperature\n4. Fill wells with Tris-buffered saline for section washing\n5. Wait 5 minutes during Tris-buffered saline wash\n6. Repeat Tris-buffered saline wash procedure twice\n7. Dilute biotinylated secondary antibody at 1:200 concentration in Tris-buffered saline with 10% horse serum\n8. Prepare required volume of secondary antibody solution based on number of sections\n9. Apply 200 microliters of secondary antibody solution to each well of slide-holder set\n10. Incubate secondary antibody-treated slides for 30 minutes at room temperature\n11. Repeat Tris-buffered saline wash after secondary antibody incubation", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7", "H. 8", "I. 9", "J. 10", "K. 11"], "answer": "G", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/64560/clip_4_removed_step_7.mp4"}} |
| {"qid": "ExpVid_5608", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Dilute whole blood with PBS in 1:1 ratio\n2. Layer diluted blood onto one volume of density gradient medium\n3. Hold tube at 45° angle during layering\n4. Centrifuge suspension at 900 G for 20 minutes at room temperature\n5. Transfer lymphocyte-containing cloudy layer to new sterile conical tube\n6. Wash lymphocytes with PBS\n7. Count lymphocytes using hemocytometer\n8. Dilute lymphocyte suspension to 800,000 cells/mL in complete RPMI\n9. Irradiate lymphocytes with gamma rays from cobalt-60 source\n10. Incubate irradiated lymphocytes at 37°C for 1 hour in humidified 5% CO₂ incubator\n11. Place slide into clip holder\n12. Place filter card onto slide assembly\n13. Place funnel onto assembly\n14. Secure slide clips\n15. Place assembly into cyto-centrifuge\n16. Add 250 microliters of cell suspension into each funnel\n17. Spin cyto-centrifuge at 30 G for five minutes\n18. Remove slides from clip assembly\n19. Circle cell spot with hydrophobic pen\n20. Fix cells with 3% paraformaldehyde (PFA) in PBS solution for 20 minutes\n21. Wash slides in Coplin jar with PBS for 5 minutes\n22. Cover cells with Triton X for permeabilization\n23. Wash slides with blocking solution (1% BSA in PBS) to reduce non-specific binding\n24. Incubate slides with 1:500 primary anti-gamma H2AX antibody at room temperature for one hour in humidifying chamber\n25. Prepare humidifying chamber by placing wet tissue paper at base of rectangular slide storage box\n26. Tip off primary antibody liquid from slides\n27. Wash slides in 1% BSA solution\n28. Incubate slides with 1:1000 diluted secondary donkey anti-mouse TRITC antibody for one hour in humidifying chamber\n29. Remove secondary antibody from slides\n30. Perform three 10-minute washes in PBS\n31. Cover Coplin jar in aluminum foil to prevent light exposure during washes\n32. Wipe excess PBS outside hydrophobic circle\nAnswer with the step number only.", "options": null, "answer": "49", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/62623/clip_9_prediction.mp4"}} |
| {"qid": "ExpVid_6067", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Prepare 0.1 M sodium bicarbonate buffer by mixing 8.4 g sodium bicarbonate and 29.2 g sodium chloride in 1 L water\n2. Adjust buffer pH to 8.4\n3. Measure 500 microliters of buffer\n4. Add 70 microliters of anti H-N-R-N-P-A1 antibody\n5. Add 35 microliters of ATTO 550 NHS labeling reagent\n6. Rotate solution at room temperature for one hour\n7. Inject solution into dialyzer\n8. Dialyze in two liters of PBS at 4°C overnight\n9. Concentrate dialyzed labeled antibody using spin column\n10. Determine antibody concentration using NanoDrop spectrophotometer\n11. Seed 10,000 cells per well in an eight-well slide with 500 microliters of complete DMEM\n12. Incubate cells at 37°C until confluency reaches at least 70%\n13. Mix 2 microliters of antibody, transfection reagents, and 2 micrograms of labeled antibody (0.5 μg/μL) in a vessel\n14. Incubate mixture for 10-15 minutes at room temperature\n15. Remove supernatant from cell culture wells\n16. Add 394 microliters of fresh medium per well\n17. Add 100 microliters of DMEM to transfection solution\n18. Mix transfection solution by pipetting up and down\n19. Add 106 microliters of transfection mixture per well\n20. Set up UNTRANSFECTED negative control well by adding DMEM instead of antibody\n21. Incubate cells at 37°C for 48 hours\n22. Replace medium with fresh DMEM\n23. Perform live imaging using fluorescent microscope with SI three filter for ATO five 50 NHS labeled antibodies\n24. Aspirate off medium\n25. Add 4% paraldehyde to fix cells\n26. Incubate slide for 15 minutes at room temperature\n27. Wash cells four times in PBS with 5-minute incubation per wash\n28. Remove PBS\n29. Add drop of mounting medium containing DAPI to each well\n30. Gently place cover slip on cells\n31. Image cells using fluorescent microscope\nAnswer with the step number only.", "options": null, "answer": "10", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/4154/clip_3_prediction.mp4"}} |
| {"qid": "ExpVid_6819", "question": "Given the following step list，which step was not performed in the video?\n1. Position filled containers in previously-constructed drilled foam within pots\n2. Mix medium particle size sand with small particle size sand in cement mixer to create nutrient-poor silica sand mixture\n3. Fill interstitial space between containers with sand mixture using funnel", "options": ["A. 1", "B. 2", "C. 3"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/59338/clip_4_removed_step_1.mp4"}} |
| {"qid": "ExpVid_5386", "question": "What is the correct sequence of steps for enzymatically digesting tumor fragments to create a cell suspension?", "options": ["A. 1. Add 1 mL of 10 mM HEPES in DMEM to tumor fragments\n2. Mince tissue with sterilized razor blade\n3. Add 9 mL of DMEM with HEPES, collagenase type 1/2, and thermolysin to tumor pieces\n4. Incubate mixture for 1.5-2 hours at 37°C and 5% CO₂ on orbital shaker", "B. 1. Mince tissue with sterilized razor blade\n2. Add 1 mL of 10 mM HEPES in DMEM to tumor fragments\n3. Add 9 mL of DMEM with HEPES, collagenase type 1/2, and thermolysin to tumor pieces\n4. Incubate mixture for 1.5-2 hours at 37°C and 5% CO₂ on orbital shaker", "C. 1. Add 1 mL of 10 mM HEPES in DMEM to tumor fragments\n2. Add 9 mL of DMEM with HEPES, collagenase type 1/2, and thermolysin to tumor pieces\n3. Mince tissue with sterilized razor blade\n4. Incubate mixture for 1.5-2 hours at 37°C and 5% CO₂ on orbital shaker", "D. 1. Add 1 mL of 10 mM HEPES in DMEM to tumor fragments\n2. Mince tissue with sterilized razor blade\n3. Incubate mixture for 1.5-2 hours at 37°C and 5% CO₂ on orbital shaker\n4. Add 9 mL of DMEM with HEPES, collagenase type 1/2, and thermolysin to tumor pieces"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/60469/clip_1.mp4"}} |
| {"qid": "ExpVid_4835", "question": "What is the correct sequence of steps for the biomass acid digestion and separation experiment?", "options": ["A. 1. Add 150 ml distilled water to 1 L round bottom flask\n2. Add 1 g dried jute biomass and 15 ml of 72 wt% sulfuric acid to 50 ml vial\n3. Stir mixture on hot plate at 30°C for 2 hours\n4. Transfer digested biomass sample to the flask\n5. Transfer wash water to flask containing digested biomass\n6. Wash vial with 195 ml water\n7. Reflux solution for 4 hours\n8. After 12 hours, filter solution using G2 crucible to obtain insoluble lignin/ash\n9. Cool round bottom flask to room temperature\n10. Wash insoluble solid with 150 ml hot water to remove residual acid", "B. 1. Add 1 g dried jute biomass and 15 ml of 72 wt% sulfuric acid to 50 ml vial\n2. Stir mixture on hot plate at 30°C for 2 hours\n3. Transfer digested biomass sample to the flask\n4. Add 150 ml distilled water to 1 L round bottom flask\n5. Wash vial with 195 ml water\n6. Transfer wash water to flask containing digested biomass\n7. Reflux solution for 4 hours\n8. Cool round bottom flask to room temperature\n9. After 12 hours, filter solution using G2 crucible to obtain insoluble lignin/ash\n10. Wash insoluble solid with 150 ml hot water to remove residual acid", "C. 1. Add 1 g dried jute biomass and 15 ml of 72 wt% sulfuric acid to 50 ml vial\n2. Add 150 ml distilled water to 1 L round bottom flask\n3. Transfer digested biomass sample to the flask\n4. Stir mixture on hot plate at 30°C for 2 hours\n5. Wash vial with 195 ml water\n6. Transfer wash water to flask containing digested biomass\n7. Reflux solution for 4 hours\n8. After 12 hours, filter solution using G2 crucible to obtain insoluble lignin/ash\n9. Cool round bottom flask to room temperature\n10. Wash insoluble solid with 150 ml hot water to remove residual acid", "D. 1. Add 1 g dried jute biomass and 15 ml of 72 wt% sulfuric acid to 50 ml vial\n2. Stir mixture on hot plate at 30°C for 2 hours\n3. Add 150 ml distilled water to 1 L round bottom flask\n4. Transfer digested biomass sample to the flask\n5. Wash vial with 195 ml water\n6. Transfer wash water to flask containing digested biomass\n7. Reflux solution for 4 hours\n8. Cool round bottom flask to room temperature\n9. After 12 hours, filter solution using G2 crucible to obtain insoluble lignin/ash\n10. Wash insoluble solid with 150 ml hot water to remove residual acid"], "answer": "D", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/57613/clip_4.mp4"}} |
| {"qid": "ExpVid_6674", "question": "Given the following step list，which step was not performed in the video?\n1. Weigh 100 grams of Taiwanese green propolis\n2. Grind propolis into fine powder using spice grinder (no large particles)\n3. Set out five flasks and add 100 milliliters of various ethanol concentrations to each\n4. Mix 10 grams of ground propolis into ethanol solution in each flask\n5. Incubate flasks at 25°C with shaking at 250 RPM for 48 hours", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/58743/clip_2_removed_step_3.mp4"}} |
| {"qid": "ExpVid_6655", "question": "Given the following step list，which step was not performed in the video?\n1. Place familiar object and novel object in opposite quadrants of arena\n2. Use same object locations as during training for each mouse\n3. Remove mouse from home cage\n4. Place mouse in center arena equidistant from familiar and novel objects\n5. Allow free exploration for 10 minutes\n6. Remove mouse from arena after testing trial\n7. Place mouse in holding cage", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5", "F. 6", "G. 7"], "answer": "F", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/55718/clip_6_removed_step_6.mp4"}} |
| {"qid": "ExpVid_4737", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Add 1 mg of fluorescently labeled compound to 100 microliters of deionized water\n2. Dissolve 65 mg of PLGA in 750 microliters of dichloromethane in scintillation vial\n3. Ultrasonicate mixture for 10-30 seconds at 160 watts to dissolve PLGA\n4. Add entire first water phase to oil phase dropwise\n5. Emulsify solution using handheld homogenizer at 20,000 rpm for 30 seconds\n6. Add entire first water-oil phase dropwise into 15 ml of 1% PVA solution\n7. Emulsify mixture as previously demonstrated\n8. Transfer entire emulsion volume into 200 milliliter round-bottom flask\n9. Remove solvent using rotary evaporator at 635 mmHg vacuum for 1 hour\n10. Aliquot 1 milliliter of aqueous phase into each of 14 microcentrifuge tubes\n11. Pellet microspheres by centrifugation\n12. Remove 900 microliters of aqueous solution from each tube using micropipette without disturbing pellets\n13. Wash microspheres with 1 milliliter de-ionized water\n14. Remove 900 microliters of supernatant carefully\n15. Seed 40 visually size-matched islets into one well of a 96-well tissue culture plate\n16. Add 200 microliters of islet culture medium to the seeded well\n17. Resuspend microspheres in pre-assay medium to achieve 10 nanograms per microliter compound concentration\n18. Sonicate microspheres in ice water bath for 10 minutes at 160 watts with 10-second pulses at 4°C\n19. Remove 100 microliters of islet culture medium from each well without dislodging islets\n20. Add 100 microliters of microsphere-containing assay medium to each well\n21. Carefully discard assay medium from all wells without disturbing islets\n22. Gently wash islets with 200 microliters of PBS (two times)\n23. Immerse islets in 100 microliters of trypsin EDTA solution\n24. Incubate islets in trypsin EDTA for 3 minutes at room temperature\n25. Perform gentle pipetting after 2 minutes of incubation\n26. Transfer entire well volume into individual microcentrifuge tu\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "8, 9, 10, 11, 12, 13, 14", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/54664/clip_3.mp4"}} |
| {"qid": "ExpVid_4070", "question": "Based on the full experimental procedure，determine the step numbers shown in the video.\n1. Grow HTT11619 cells in 25 ml of prepared medium in T-175 flask\n2. Aspirate culture medium from T-175 flask\n3. Wash cells with PBS without calcium and magnesium\n4. Aspirate PBS from T-175 flask\n5. Add trypsin drop-wise to T-175 flask using 5 ml pipette\n6. Incubate flask to allow cell detachment\n7. Tap flask to dislodge cells\n8. Quench cells with 8 ml complete medium over flask surface\n9. Pipette mixture up and down to break cell clumps\n10. Transfer cell mixture to 15 ml conical tube\n11. Take 10 microliters from 15 milliliter conical tube\n12. Combine sample with 10 microliters of trypan blue\n13. Transfer 10 microliters of cell-trypan blue mixture to hemocytometer\n14. Count cells in four outer grids of hemocytometer\n15. Add 5 milliliters of complete medium and 6 micromolar aphidicolin to each 10-centimeter plate of cells\n16. Transfer 100 microliters of re-suspended cell pellet to each centimeter plate\n17. Swirl plate gently to mix\n18. Incubate plates to synchronize cells at G1/S border\n19. Mix RNA components to 45 micromolar concentration in equal molar concentrations\n20. Add 6.75 microliters of 200 micromolar CR RNA stock to 1.5 mL centrifuge tube\n21. Add 6.75 microliters of 200 micromolar tracer RNA stock to centrifuge tube\n22. Add 16.50 microliters of IDT buffer to centrifuge tube\n23. Heat mixture at 95°C for 5 minutes in PCR machine\n24. Cool samples to room temperature\n25. Dilute 2.22 microliters of CR RNA complex and 2.78 microliters of IDT buffer to final volume of 5 microliters\n26. Dilute 1.67 microliters of Cas9 protein (60 micromolar stock) in 3.33 microliters of low sera medium to final volume of 5 microliters\n27. Mix 5 microliters of Cas9 protein with 5 microliters of complex RNA\n28. Aspirate medium from plate\n29. Wash plate with 5 ml of PBS\n30. Aspirate PBS from plate\n31. Add 1 ml pre-warmed trypsin to each 10 cm plate\n32. Place plates in incubator\n33. Tap 10 cm plate\nAnswer with the step numbers only, separated by commas.", "options": null, "answer": "15, 16, 17, 18", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/56195/clip_3.mp4"}} |
| {"qid": "ExpVid_6980", "question": "Given the following step list，which step was not performed in the video?\n1. Aspirate old media from cells\n2. Add 1 milliliter of sterile room-temperature PBS to each well\n3. Detach cells from well bottom by pipetting PBS up and down\n4. Transfer cell mixture to 5 milliliter snap cap tubes\n5. Immediately analyze cells via flow cytometry", "options": ["A. 1", "B. 2", "C. 3", "D. 4", "E. 5"], "answer": "C", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_verification/2355/clip_6_removed_step_3.mp4"}} |
| {"qid": "ExpVid_7211", "question": "In the described assay, invasion of human endothelial cells by _____ requires _____; mutants lacking these show markedly reduced internalization, and invasion is restored only by complementation with _____, not with a version missing the _____.", "options": null, "answer": "Staphylococcus aureus | fibronectin-binding proteins | full-length FnBPA | fibronectin-binding domain", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/2693_.mp4"}} |
| {"qid": "ExpVid_7464", "question": "According to the study’s conclusion, this method establishes a ____ insulin secretion assay that employs ratiometric ____/____ nm detection to accurately quantify ____ and enable high-throughput analysis of pancreatic beta-cell function.", "options": null, "answer": "HTRF | 665 | 620 | glucose-stimulated insulin secretion", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/57531_.mp4"}} |
| {"qid": "ExpVid_5325", "question": "What is the correct sequence of steps for the experimental procedure titled 'Freeze-thaw cycles and decalcification'?", "options": ["A. 1. Thaw frozen bones at room temperature\n2. Place bones in sterile 50 milliliter centrifuge tube\n3. Freeze bones at -80 degrees Celsius for one hour\n4. Subject bones to more than two freeze-thaw cycles for cell lysis and tissue breakdown\n5. Fill tube with 0.5 normal HCl\n6. Incubate overnight at room temperature on orbital shaker with gentle shaking\n7. Decant hydrochloric acid solution completely\n8. Rinse bones under running water for one hour\n9. Wash bones with distilled water for 15 minutes on orbital shaker\n10. Completely decant solution", "B. 1. Thaw frozen bones at room temperature\n2. Freeze bones at -80 degrees Celsius for one hour\n3. Subject bones to more than two freeze-thaw cycles for cell lysis and tissue breakdown\n4. Place bones in sterile 50 milliliter centrifuge tube\n5. Fill tube with 0.5 normal HCl\n6. Incubate overnight at room temperature on orbital shaker with gentle shaking\n7. Decant hydrochloric acid solution completely\n8. Rinse bones under running water for one hour\n9. Wash bones with distilled water for 15 minutes on orbital shaker\n10. Completely decant solution", "C. 1. Thaw frozen bones at room temperature\n2. Freeze bones at -80 degrees Celsius for one hour\n3. Place bones in sterile 50 milliliter centrifuge tube\n4. Subject bones to more than two freeze-thaw cycles for cell lysis and tissue breakdown\n5. Fill tube with 0.5 normal HCl\n6. Incubate overnight at room temperature on orbital shaker with gentle shaking\n7. Decant hydrochloric acid solution completely\n8. Rinse bones under running water for one hour\n9. Wash bones with distilled water for 15 minutes on orbital shaker\n10. Completely decant solution", "D. 1. Thaw frozen bones at room temperature\n2. Freeze bones at -80 degrees Celsius for one hour\n3. Subject bones to more than two freeze-thaw cycles for cell lysis and tissue breakdown\n4. Place bones in sterile 50 milliliter centrifuge tube\n5. Fill tube with 0.5 normal HCl\n6. Incubate overnight at room temperature on orbital shaker with gentle shaking\n7. Decant hydrochloric acid solution completely\n8. Wash bones with distilled water for 15 minutes on orbital shaker\n9. Completely decant solution\n10. Rinse bones under running water for one hour"], "answer": "B", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/59271/clip_2.mp4"}} |
| {"qid": "ExpVid_5065", "question": "What is the correct sequence of steps for imaging LC3-positive autophagosome formation in transfected HEC293T cells?", "options": ["A. 1. Prepare incubation chamber\n2. Place incubation chamber on microscope stage\n3. Replace rich medium with starvation medium\n4. Induce autophagy response\n5. Select appropriate cells for imaging\n6. Set video microscopy parameters", "B. 1. Prepare incubation chamber\n2. Replace rich medium with starvation medium\n3. Place incubation chamber on microscope stage\n4. Set video microscopy parameters\n5. Select appropriate cells for imaging\n6. Induce autophagy response", "C. 1. Place incubation chamber on microscope stage\n2. Prepare incubation chamber\n3. Replace rich medium with starvation medium\n4. Induce autophagy response\n5. Set video microscopy parameters\n6. Select appropriate cells for imaging", "D. 1. Prepare incubation chamber\n2. Place incubation chamber on microscope stage\n3. Induce autophagy response\n4. Replace rich medium with starvation medium\n5. Select appropriate cells for imaging\n6. Set video microscopy parameters"], "answer": "A", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/video_segments/50484/clip_1.mp4"}} |
| {"qid": "ExpVid_5933", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Position anesthetized mouse in stereotactic platform with incisors hooked in bite bar\n2. Tighten nose clamp while maintaining mouse head on level plane\n3. Adjust ear bar height to reach caudal portion of ear canal for head immobilization\n4. Disinfect head with alternating wipes of topical antiseptic and 70% ethanol\n5. Make 0.75 centimeter horizontal incision in mid scalp using sterile scalpel\n6. Drill two symmetrical holes using 0.45 millimeter burr: positioned above right/left cortical hemispheres, 2 mm from sagittal suture and lambdoid suture at middle of parietal bone\n7. Attach 10 microliter syringe to stereotactic platform\n8. Load 10 microliters of freshly prepared chemical inhibitor into syringe\n9. Advance needle perpendicularly into first burr hole\n10. Zero coordinates on stereotactic digital display after needle traverses skull\n11. Advance needle tip to 2.5 millimeters depth\n12. Withdraw needle 0.5 millimeters to 2 millimeters depth\n13. Slowly inject entire 10 microliter volume over one minute\n14. Leave needle in brain for one minute after injection completion\n15. Repeat injection procedure in second burr hole using vehicle only\n16. Close skin over incision\n17. Transfer mouse from stereotactic apparatus to 37°C heating pad\n18. Monitor mouse until full recovery\n19. Secure mouse on dissecting pad\n20. Make lateral incision through integument and abdominal wall beneath ribcage using scissors and forceps\n21. Separate liver from diaphragm\n22. Cut diaphragm along entire ribcage length to expose pleural cavity\n23. Make incision to posterior end of left ventricle using scissors\n24. Perfuse right heart chamber with 15 ml PBS over 2 minutes\n25. Perfuse heart with 10 ml of 4% paraformaldehyde in PBS over 2 minutes\n26. Make midline incision in scalp using scissors\n27. Place scissors tip into foramen magnum to c\nAnswer with the step number only.", "options": null, "answer": "34", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/59449/clip_5_prediction.mp4"}} |
| {"qid": "ExpVid_6230", "question": "Given the complete step list of the experiment, please predict the next step that will take place after experimental steps shown in this video.\nComplete step list: Add 20 microliters of freshly prepared POPC solution and 4 microliters of freshly prepared biotin-PEG-DSPE solution to a glass vial\n2. Evaporate organic solvent by air flow until lipid film forms\n3. Place lipid film in desiccator for one hour to completely evaporate organic solvent\n4. Add 200 microliters of mineral oil to the vial\n5. Cover vial opening with plastic paraffin film\n6. Sonicate vial contents in ultrasonic bath at 120 watts for at least one hour\n7. Create a lipid film\n8. Add 200 microliters of 200 millimolar glucose in LB medium to the lipid film\n9. Sonicate the film at 120 watts for at least one hour\n10. Extrude resulting giant vesicles into small vesicles using a mini extruder and polycarbonate membrane with 100-nanometer pore size\n11. Inoculate E. coli from LB plate into LB medium\n12. Incubate culture overnight at 37°C\n13. Transfer 20 microliters of culture supernatant to 1.98 milliliters of fresh LB medium\n14. Incubate sub-culture for two hours\n15. Measure optical density at 600 nm (OD600) using spectrophotometer\n16. Mix pre-culture solution with sucrose solution and fresh LB medium according to table one\n17. Add 50 microliters of freshly prepared outer aqueous solution to a 1.5 milliliter lidded plastic tube\n18. Layer 150 microliters of lipid-containing oil solution over the outer aqueous solution\n19. Incubate solution for 10 to 15 minutes at room temperature\n20. Verify oil-aqueous solution interface is flat\n21. Add 2 microliters of freshly prepared inner aqueous solution of giant vesicles to 50 microliters of sonicated lipid-containing oil solution in 0.6 milliliter lidded plastic tube\n22. Tap tube to emulsify components\n23. Use pipette to add 50 microliters of water and oil droplet solution to oil-aqueous solution interface\n24. Sediment bacterial cell-containing giant vesicles by centri\nAnswer with the step number only.", "options": null, "answer": "20", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_2/step_prediction/59555/clip_5_prediction.mp4"}} |
| {"qid": "ExpVid_7649", "question": "The methodological innovation described is a modular tissue engineering approach that fabricates ____-coated ____ small enough to avoid oxygen-diffusion constraints, and allows assembly and study in ____ and in vivo to promote ____.", "options": null, "answer": "endothelial cell–coated | collagen gel modules | microfluidic chambers | rapid vascularization", "subcategory": "_none", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_3/2177_.mp4"}} |
| {"qid": "ExpVid_1096", "question": "Which material appears in this experimental step?", "options": ["A. thermistor probe", "B. cold presser arm wrap", "C. elastic bandage", "D. blood pressure cuff"], "answer": "B", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50849/clip_4.mp4"}} |
| {"qid": "ExpVid_2991", "question": "Which tool is being utilized in this experimental technique?", "options": ["A. stereotactic system", "B. brain atlas manipulator", "C. animal restraint device", "D. surgical head holder"], "answer": "A", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/52653/clip_39.mp4"}} |
| {"qid": "ExpVid_2598", "question": "What is the voltage of the lithium cell battery?", "options": ["A. 5V", "B. 9V", "C. 1.5V", "D. 3V"], "answer": "D", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55098/clip_18.mp4"}} |
| {"qid": "ExpVid_1156", "question": "Which material appears in this experimental step?", "options": ["A. 70% ethanol", "B. disinfecting detergent", "C. sodium hypochlorite (bleach)", "D. liquid hand soap"], "answer": "B", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53009/clip_24.mp4"}} |
| {"qid": "ExpVid_1708", "question": "What is the person doing with the cold presser arm wrap?", "options": ["A. Adjusting its position on the arm", "B. Visually inspecting its exterior condition", "C. Measuring its surface temperature with a tool", "D. Removing it from the patient"], "answer": "C", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50849/clip_3.mp4"}} |
| {"qid": "ExpVid_26", "question": "Which material appears in this experimental step?", "options": ["A. tubulin-Gal4 larvae", "B. white-eyed mutant larvae", "C. breathless Gal4 experimental larvae", "D. wild-type Drosophila larvae"], "answer": "C", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57131/clip_67.mp4"}} |
| {"qid": "ExpVid_2129", "question": "What is the person doing with the battery pack?", "options": ["A. Connecting it to the converter module via the two-pin connection", "B. Attaching it to the converter's output terminals instead of input", "C. Securing it with the nut instead of connecting it", "D. Disconnecting it from the two-pin port after testing"], "answer": "A", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55098/clip_39.mp4"}} |
| {"qid": "ExpVid_961", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. 1% agar gel", "B. agarose gel", "C. gelatin gel", "D. polyacrylamide gel"], "answer": "A", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62533/clip_19.mp4"}} |
| {"qid": "ExpVid_2772", "question": "What depth of corncob bedding is visible in the bin?", "options": ["A. 0.5-1 cm", "B. 3-3.5 cm", "C. 2-2.5 cm", "D. 1.5-2 cm"], "answer": "C", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/52874/clip_19.mp4"}} |
| {"qid": "ExpVid_1480", "question": "What is the researcher timing with the stopwatch?", "options": ["A. The animal traversing the entire U-shaped channel", "B. The animal entering the U-shaped channel", "C. The animal entering the goal box", "D. The placement of the obstruction in the channel"], "answer": "C", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/52225/clip_30.mp4"}} |
| {"qid": "ExpVid_218", "question": "What material appears in this procedure?", "options": ["A. microorganisms", "B. cell cultures", "C. tissue samples", "D. animals"], "answer": "D", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53010/clip_8.mp4"}} |
| {"qid": "ExpVid_3634", "question": "Which tool is being used in this experimental step?", "options": ["A. foot switch", "B. circuit breaker", "C. trigger button", "D. push-button switch"], "answer": "A", "subcategory": "research/behavior", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53367/clip_14.mp4"}} |
| {"qid": "ExpVid_1693", "question": "What is the researcher doing with the microscope?", "options": ["A. Adjusting the focus on the objective lens", "B. Calibrating the laser power output", "C. Capturing fluorescent images at timed intervals", "D. Changing the emission filter settings"], "answer": "C", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/67076/clip_67.mp4"}} |
| {"qid": "ExpVid_3620", "question": "Which scientific instrument is being employed in this experiment?", "options": ["A. 24-well plate", "B. Petri dish", "C. 48-well plate", "D. 96-well plate"], "answer": "C", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60461/clip_11.mp4"}} |
| {"qid": "ExpVid_53", "question": "Which material appears in this experimental step?", "options": ["A. microscope slide", "B. prepared flow cell", "C. reaction chamber", "D. syringe reservoir"], "answer": "B", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/63501/clip_17.mp4"}} |
| {"qid": "ExpVid_564", "question": "Which material appears in this experimental step?", "options": ["A. total eluate", "B. column wash buffer", "C. binding buffer", "D. flow-through"], "answer": "A", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/64923/clip_37.mp4"}} |
| {"qid": "ExpVid_2679", "question": "What volume is set on the pipette?", "options": ["A. 10 µL", "B. 20 µL", "C. 15 µL", "D. 5 µL"], "answer": "A", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58334/clip_40.mp4"}} |
| {"qid": "ExpVid_3198", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. microcentrifuge", "B. magnetic stirrer", "C. tube centrifuge rack", "D. magnetic rack"], "answer": "D", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/67076/clip_47.mp4"}} |
| {"qid": "ExpVid_1092", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. blank solution", "B. diluted sample solution", "C. phosphate buffer solution", "D. diluted standard compound"], "answer": "D", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57293/clip_24.mp4"}} |
| {"qid": "ExpVid_1179", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. nylon membrane", "B. glass fiber membrane", "C. PVDF membrane", "D. nitrocellulose membrane"], "answer": "B", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62754/clip_44.mp4"}} |
| {"qid": "ExpVid_812", "question": "Which material appears in this experimental step?", "options": ["A. 5% oxygen", "B. carbon monoxide", "C. helium", "D. 5% carbon dioxide"], "answer": "D", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60461/clip_10.mp4"}} |
| {"qid": "ExpVid_4011", "question": "What laboratory tool is being used in this research step?", "options": ["A. cryovial", "B. Eppendorf tube", "C. vial", "D. microcentrifuge tube"], "answer": "C", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61054/clip_18.mp4"}} |
| {"qid": "ExpVid_2148", "question": "What is the person doing with the pipette and the SEM buffer?", "options": ["A. Swirling it in the protein tube", "B. Adding it to the protein solution", "C. Measuring its concentration in the tube", "D. Removing it from the protein solution"], "answer": "B", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62853/clip_12.mp4"}} |
| {"qid": "ExpVid_1018", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. hydrogen peroxide", "B. catalase", "C. albumin", "D. hydrogenase"], "answer": "D", "subcategory": "research/biochemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55858/clip_11.mp4"}} |
| {"qid": "ExpVid_321", "question": "Which material appears in this experimental step?", "options": ["A. Tris buffer", "B. PBS-AA", "C. heparinized saline", "D. PBS-Tween"], "answer": "B", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50658/clip_19.mp4"}} |
| {"qid": "ExpVid_1923", "question": "What is the person doing with the spray bottle?", "options": ["A. Wiping the model with a lacquer-dampened cloth", "B. Spraying the model with lacquer", "C. Brushing lacquer onto the model", "D. Pouring lacquer over the model"], "answer": "B", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62279/clip_8.mp4"}} |
| {"qid": "ExpVid_2844", "question": "What volume of protein sample is applied?", "options": ["A. 5 µL", "B. 10 µL", "C. 2 µL", "D. 1 µL"], "answer": "C", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3227/clip_45.mp4"}} |
| {"qid": "ExpVid_681", "question": "What material appears in this procedure?", "options": ["A. collagen hydrogel", "B. Matrigel", "C. kidney ECM hydrogel", "D. alginate hydrogel"], "answer": "C", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58314/clip_37.mp4"}} |
| {"qid": "ExpVid_3894", "question": "What tool is being used by the researcher in this video clip?", "options": ["A. culture plate", "B. microscope slide", "C. specimen stub", "D. grid"], "answer": "D", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3227/clip_45.mp4"}} |
| {"qid": "ExpVid_126", "question": "What material appears in this procedure?", "options": ["A. epithelial cells", "B. endothelial cells", "C. red blood cells", "D. fibroblasts"], "answer": "B", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/2177/clip_47.mp4"}} |
| {"qid": "ExpVid_3093", "question": "What tool is being used by the researcher in this video clip?", "options": ["A. centrifuge", "B. microcentrifuge", "C. vortex mixer", "D. rotator/mixer"], "answer": "A", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61691/clip_46.mp4"}} |
| {"qid": "ExpVid_1901", "question": "What is the person doing with the pipette after counting the cells?", "options": ["A. Pouring cells into the hemocytometer", "B. Transferring cells to a conical tube", "C. Removing excess medium from the tube", "D. Placing the conical tube on ice"], "answer": "B", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/51044/clip_31.mp4"}} |
| {"qid": "ExpVid_2654", "question": "What temperature increase is shown on the device?", "options": ["A. 5°C", "B. 20°C", "C. 15°C", "D. 10°C"], "answer": "D", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61184/clip_29.mp4"}} |
| {"qid": "ExpVid_320", "question": "Which material appears in this experimental step?", "options": ["A. SDS solution (1% sodium dodecyl sulfate)", "B. distilled water", "C. running deionized water", "D. saline solution (0.9% NaCl)"], "answer": "C", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50658/clip_30.mp4"}} |
| {"qid": "ExpVid_3522", "question": "What laboratory tool is being used in this research step?", "options": ["A. microcentrifuge tube", "B. PCR tube", "C. cryovial", "D. conical tube"], "answer": "A", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/54664/clip_25.mp4"}} |
| {"qid": "ExpVid_2329", "question": "What duration is set on the centrifuge?", "options": ["A. 10 minutes", "B. 15 minutes", "C. 12 minutes", "D. 20 minutes"], "answer": "C", "subcategory": "research/bioengineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61691/clip_26.mp4"}} |
| {"qid": "ExpVid_3672", "question": "What laboratory equipment is being utilized to cover the feeding cage in this procedure?", "options": ["A. filter paper", "B. foam mat", "C. foam plug", "D. petri dish lid"], "answer": "B", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/63020/clip_14.mp4"}} |
| {"qid": "ExpVid_2147", "question": "What is the person doing with the pipette containing cell suspension?", "options": ["A. Mixing the suspension in the reservoir before transfer", "B. Transferring 500 microliters to an uncoated petri dish", "C. Removing 500 microliters from the coated coverslip", "D. Dispensing 500 microliters onto a coated coverslip"], "answer": "D", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/201062/clip_9.mp4"}} |
| {"qid": "ExpVid_315", "question": "Which material appears in this experimental step?", "options": ["A. 10 milligrams per milliliter salmon testes carrier DNA", "B. Herring sperm carrier DNA", "C. TE buffer (10 mM Tris, 1 mM EDTA)", "D. Salmon sperm DNA"], "answer": "A", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/51551/clip_16.mp4"}} |
| {"qid": "ExpVid_2279", "question": "How many clips is the substance divided into?", "options": ["A. 16", "B. 10", "C. 14", "D. 12"], "answer": "D", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/200904/clip_23.mp4"}} |
| {"qid": "ExpVid_1535", "question": "What is the person doing with the pipette and cell suspension?", "options": ["A. Pouring the suspension onto the dish surface", "B. Measuring suspension volume in the dish", "C. Removing old media from the dish", "D. Transferring the suspension into a petri dish"], "answer": "D", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58081/clip_4.mp4"}} |
| {"qid": "ExpVid_105", "question": "Which material appears in this experimental step?", "options": ["A. saline solution", "B. non-motile sperm", "C. motile sperm", "D. methylene blue"], "answer": "C", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62432/clip_41.mp4"}} |
| {"qid": "ExpVid_2385", "question": "What volume of hybridization solution is used to dissolve the RNA pellet?", "options": ["A. 60 microliters", "B. 70 microliters", "C. 50 microliters", "D. 80 microliters"], "answer": "A", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3250/clip_20.mp4"}} |
| {"qid": "ExpVid_3734", "question": "Which tool is being utilized in this experimental technique?", "options": ["A. primer plate", "B. indexing beads", "C. library index kit", "D. multiplex PCR kit"], "answer": "C", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58239/clip_30.mp4"}} |
| {"qid": "ExpVid_451", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. femoral artery", "B. aorta", "C. vena cava", "D. trachea"], "answer": "B", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/51357/clip_26.mp4"}} |
| {"qid": "ExpVid_1982", "question": "What is the person doing with the transfection complex?", "options": ["A. Measuring its volume in a tube", "B. Mixing it with other solutions", "C. Adding it to cells", "D. Removing it from cells"], "answer": "C", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50484/clip_14.mp4"}} |
| {"qid": "ExpVid_2561", "question": "What volume of buffer is added?", "options": ["A. 1.5 mL", "B. 0.5 mL", "C. 1 mL", "D. 2 mL"], "answer": "C", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57629/clip_43.mp4"}} |
| {"qid": "ExpVid_3364", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. sample loading chamber", "B. anode buffer reservoir", "C. cathode buffer reservoir", "D. buffer chamber"], "answer": "C", "subcategory": "research/biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/1842/clip_22.mp4"}} |
| {"qid": "ExpVid_2053", "question": "What is the person doing with the hydrochloric acid solution?", "options": ["A. Diluting the solution with water", "B. Stirring the solution", "C. Pouring the solution into a new container", "D. Decanting the solution"], "answer": "D", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/59271/clip_13.mp4"}} |
| {"qid": "ExpVid_3470", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. centrifuge tube", "B. conical tube", "C. culture tube", "D. test tube"], "answer": "C", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60273/clip_20.mp4"}} |
| {"qid": "ExpVid_2872", "question": "What volume of HBSSS is used to resuspend the cell pellet?", "options": ["A. 5 mL", "B. 15 mL", "C. 20 mL", "D. 10 mL"], "answer": "D", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/64216/clip_13.mp4"}} |
| {"qid": "ExpVid_3094", "question": "What laboratory tool is being used in this research step?", "options": ["A. T25 cell culture flask", "B. six-well tissue culture plate", "C. glass microscope slide", "D. 96-well microplate"], "answer": "B", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/64889/clip_23.mp4"}} |
| {"qid": "ExpVid_522", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. 2% vaporized isoflurane", "B. ketamine", "C. sevoflurane", "D. vaporized ethanol"], "answer": "A", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60469/clip_37.mp4"}} |
| {"qid": "ExpVid_407", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. microbeads", "B. spheroids", "C. organoids", "D. Matrigel"], "answer": "B", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58359/clip_17.mp4"}} |
| {"qid": "ExpVid_200", "question": "What material appears in this procedure?", "options": ["A. primary anti-gamma H2AX antibody", "B. normal goat serum", "C. goat anti-mouse IgG secondary antibody", "D. BSA blocking solution"], "answer": "A", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62623/clip_27.mp4"}} |
| {"qid": "ExpVid_2674", "question": "How many screws are used to secure the eyehole?", "options": ["A. 4", "B. 1", "C. 2", "D. 3"], "answer": "B", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56418/clip_14.mp4"}} |
| {"qid": "ExpVid_253", "question": "Which material appears in this experimental step?", "options": ["A. collagen solution", "B. extracellular matrix gel", "C. basement membrane matrix", "D. trypsin-EDTA"], "answer": "C", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/64889/clip_35.mp4"}} |
| {"qid": "ExpVid_638", "question": "Which material appears in this experimental step?", "options": ["A. lumen versus basal organoids", "B. fibroblast spheroids", "C. endothelial organoids", "D. Matrigel"], "answer": "A", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/63855/clip_40.mp4"}} |
| {"qid": "ExpVid_1328", "question": "What is the person doing with the fine tweezers?", "options": ["A. Placing coriands onto embryos", "B. Removing coriands from embryos", "C. Adjusting the position of embryos", "D. Measuring distance between coriands"], "answer": "B", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55459/clip_13.mp4"}} |
| {"qid": "ExpVid_2980", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. vortex mixer", "B. microplate spinner", "C. microfuge", "D. centrifuge"], "answer": "D", "subcategory": "research/cancer-research", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61449/clip_11.mp4"}} |
| {"qid": "ExpVid_1794", "question": "What is the researcher doing with the reaction mixture?", "options": ["A. Stirring it at high temperature", "B. Monitoring temperature behind shield", "C. Heating it without stirring", "D. Shaking the capped vessel"], "answer": "A", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53789/clip_31.mp4"}} |
| {"qid": "ExpVid_3884", "question": "Which tool is being utilized in this experimental technique?", "options": ["A. chromatography column", "B. round-bottom reaction flask", "C. fritted medium porosity synthesis vessel", "D. Büchner funnel"], "answer": "C", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57908/clip_3.mp4"}} |
| {"qid": "ExpVid_2827", "question": "What is the volume of water in the flask?", "options": ["A. 60 mL", "B. 100 mL", "C. 40 mL", "D. 50 mL"], "answer": "D", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53248/clip_20.mp4"}} |
| {"qid": "ExpVid_2717", "question": "What mass of sodium hydroxide is shown on the balance?", "options": ["A. 8.39 grams", "B. 9.93 grams", "C. 9.89 grams", "D. 9.39 grams"], "answer": "D", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60500/clip_42.mp4"}} |
| {"qid": "ExpVid_3223", "question": "What tool is being used by the researcher in this video clip?", "options": ["A. capillary", "B. Optical fiber", "C. Hypodermic needle", "D. Micropipette tip"], "answer": "A", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58644/clip_9.mp4"}} |
| {"qid": "ExpVid_2104", "question": "What is the person doing with the shell vials?", "options": ["A. Positioning them above a container with tweezers", "B. Inserting them into a container using tweezers", "C. Placing them sideways into the container by hand", "D. Touching them to the container's rim with tweezers"], "answer": "B", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/63779/clip_37.mp4"}} |
| {"qid": "ExpVid_814", "question": "What material appears in this procedure?", "options": ["A. carbon black", "B. silicon carbide", "C. carbon", "D. iron oxide"], "answer": "C", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58438/clip_33.mp4"}} |
| {"qid": "ExpVid_3171", "question": "What laboratory tool is being used in this research step?", "options": ["A. double-tipped transfer cannula", "B. airtight syringe", "C. burette", "D. graduated pipette"], "answer": "B", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53789/clip_7.mp4"}} |
| {"qid": "ExpVid_1074", "question": "What material appears in this procedure?", "options": ["A. THF", "B. acetonitrile", "C. DCM", "D. chloroform"], "answer": "C", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57908/clip_26.mp4"}} |
| {"qid": "ExpVid_3225", "question": "What equipment is being used to assist in adjusting the emitter-to-mass spectrometer distance?", "options": ["A. camera", "B. laser pointer", "C. webcam", "D. digital microscope"], "answer": "A", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58644/clip_30.mp4"}} |
| {"qid": "ExpVid_2282", "question": "What volume of liquid is added to the test tube?", "options": ["A. 40 mL", "B. 20 mL", "C. 30 mL", "D. 50 mL"], "answer": "C", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58882/clip_14.mp4"}} |
| {"qid": "ExpVid_401", "question": "What material appears in this procedure?", "options": ["A. Argon atmosphere", "B. Oxygen atmosphere", "C. Nitrogen atmosphere", "D. Helium atmosphere"], "answer": "C", "subcategory": "research/chemistry", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/59155/clip_20.mp4"}} |
| {"qid": "ExpVid_2726", "question": "How many pairs of forceps are used?", "options": ["A. 4", "B. 2", "C. 1", "D. 3"], "answer": "B", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56779/clip_23.mp4"}} |
| {"qid": "ExpVid_570", "question": "Which material appears in this experimental step?", "options": ["A. floating ovaries", "B. cover slips", "C. testes tissue", "D. agarose beads"], "answer": "A", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56779/clip_33.mp4"}} |
| {"qid": "ExpVid_3357", "question": "Which tool is being utilized in this experimental technique?", "options": ["A. Pasteur pipette", "B. micropipette", "C. one milliliter pipette", "D. syringe"], "answer": "C", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57759/clip_8.mp4"}} |
| {"qid": "ExpVid_941", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. buccal cavity", "B. dissection probe", "C. anal opening", "D. pharyngeal opening"], "answer": "D", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55594/clip_22.mp4"}} |
| {"qid": "ExpVid_672", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. follicular fluid", "B. collagenase solution", "C. oocyte", "D. ovarian tissue surrounding the follicle"], "answer": "D", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61625/clip_20.mp4"}} |
| {"qid": "ExpVid_3396", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. syringe needle", "B. pipette", "C. pipette tip", "D. microcentrifuge tube tip"], "answer": "C", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61954/clip_23.mp4"}} |
| {"qid": "ExpVid_3913", "question": "Which tool is being used in this experimental step?", "options": ["A. syringe", "B. pipette", "C. dropper", "D. burette"], "answer": "B", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/54934/clip_19.mp4"}} |
| {"qid": "ExpVid_2816", "question": "What volume of DCV stain is added?", "options": ["A. 6 μL", "B. 7 μL", "C. 10 μL", "D. 5 μL"], "answer": "A", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61666/clip_18.mp4"}} |
| {"qid": "ExpVid_1502", "question": "What is the person doing with the pipette?", "options": ["A. Wiping the pipette tip against the slide edge", "B. Dispensing 100 microliters of solution onto a slide", "C. Applying solution to the laboratory countertop", "D. Removing excess liquid from the slide surface"], "answer": "B", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60982/clip_27.mp4"}} |
| {"qid": "ExpVid_3179", "question": "Which laboratory apparatus is being used in this experimental step?", "options": ["A. fluorescence illumination unit", "B. UV transilluminator", "C. brightfield illuminator", "D. halogen lamp"], "answer": "A", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/200206/clip_7.mp4"}} |
| {"qid": "ExpVid_2526", "question": "How many ice buckets are used to store the equipment?", "options": ["A. 2", "B. 3", "C. 4", "D. 1"], "answer": "D", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/201777/clip_31.mp4"}} |
| {"qid": "ExpVid_199", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. phosphate-buffered saline", "B. sodium bicarbonate solution", "C. saline solution", "D. sodium chloride solution"], "answer": "D", "subcategory": "research/developmental-biology", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57847/clip_7.mp4"}} |
| {"qid": "ExpVid_2094", "question": "What is the person doing with the glue?", "options": ["A. Applying adhesive to the stir bar surface only", "B. Attaching test pieces to a triangular stir bar", "C. Joining the two test pieces together before placement", "D. Securing the stir bar to a magnetic plate"], "answer": "B", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60711/clip_14.mp4"}} |
| {"qid": "ExpVid_2453", "question": "How many petri dishes are used?", "options": ["A. 4", "B. 2", "C. 3", "D. 1"], "answer": "B", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60245/clip_17.mp4"}} |
| {"qid": "ExpVid_311", "question": "Which material appears in this experimental step?", "options": ["A. acetic acid", "B. ethanol", "C. isopropanol", "D. acetone"], "answer": "D", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/54030/clip_26.mp4"}} |
| {"qid": "ExpVid_911", "question": "Which material appears in this experimental step?", "options": ["A. photoresist remover", "B. acetone", "C. photoresist", "D. developer"], "answer": "D", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61013/clip_19.mp4"}} |
| {"qid": "ExpVid_238", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. DGAs deionized water", "B. Mineral oil", "C. Distilled water", "D. Glycerol"], "answer": "A", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/4308/clip_37.mp4"}} |
| {"qid": "ExpVid_3831", "question": "Which laboratory apparatus is being used in this experimental step?", "options": ["A. Kapton tape", "B. copper tape", "C. conductive epoxy", "D. aluminum foil"], "answer": "B", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/52818/clip_24.mp4"}} |
| {"qid": "ExpVid_2195", "question": "What is the person doing with the micropipette?", "options": ["A. Placing liquid into a round-bottomed flask", "B. Removing liquid from the flask", "C. Pouring liquid from a beaker into the flask", "D. Injecting liquid into the flask with a syringe"], "answer": "A", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53876/clip_6.mp4"}} |
| {"qid": "ExpVid_3484", "question": "What equipment is being used in this scientific procedure?", "options": ["A. thermocouple wire", "B. nickel chromium wire", "C. copper wire", "D. heating element"], "answer": "B", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/54661/clip_41.mp4"}} |
| {"qid": "ExpVid_262", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. polypropylene", "B. fluorocarbon polymer", "C. polytetrafluoroethylene", "D. Teflon"], "answer": "B", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60711/clip_14.mp4"}} |
| {"qid": "ExpVid_3020", "question": "Which laboratory apparatus is being used in this experimental step?", "options": ["A. chill plate", "B. molding flask", "C. brass plate", "D. steel plate"], "answer": "C", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50459/clip_48.mp4"}} |
| {"qid": "ExpVid_3019", "question": "What laboratory equipment is being utilized in this fiber threading procedure?", "options": ["A. microcentrifuge tube cap", "B. ferrule connector", "C. rtv end cap", "D. silicone stopper"], "answer": "C", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50459/clip_50.mp4"}} |
| {"qid": "ExpVid_2236", "question": "What volume of acrylamide solution is added?", "options": ["A. 2.1 mL", "B. 3.0 mL", "C. 1.5 mL", "D. 2.0 mL"], "answer": "A", "subcategory": "research/engineering", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/4308/clip_31.mp4"}} |
| {"qid": "ExpVid_3796", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. growth chamber", "B. autoclave", "C. water bath", "D. incubator"], "answer": "D", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/64400/clip_13.mp4"}} |
| {"qid": "ExpVid_3768", "question": "Which laboratory apparatus is being used in this experimental step?", "options": ["A. wood burning tool", "B. conductive epoxy applicator", "C. soldering iron", "D. hot air gun"], "answer": "C", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/59447/clip_16.mp4"}} |
| {"qid": "ExpVid_3674", "question": "Which tool is being used in this experimental step?", "options": ["A. AGM battery", "B. Bench power supply", "C. Lead-acid battery", "D. Deep cycle marine battery"], "answer": "A", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57928/clip_24.mp4"}} |
| {"qid": "ExpVid_1165", "question": "What material appears in this procedure?", "options": ["A. pelleted soil surface", "B. cell culture pellet", "C. agar plate surface", "D. silica gel beads"], "answer": "A", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/64759/clip_22.mp4"}} |
| {"qid": "ExpVid_2715", "question": "What volume of deionized water is pipetted?", "options": ["A. 1.5 mL", "B. 0.5 mL", "C. 1 mL", "D. 2 mL"], "answer": "C", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56074/clip_6.mp4"}} |
| {"qid": "ExpVid_1482", "question": "What is the person doing with the stopcock?", "options": ["A. Opening it to draw ambient air into sample lines", "B. Adjusting it to regulate gas flow rate", "C. Closing it to isolate the sample lines", "D. Removing it to access the manifold connection"], "answer": "A", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57332/clip_29.mp4"}} |
| {"qid": "ExpVid_1483", "question": "What is the person doing with the plastic container?", "options": ["A. Removing it from the environmental chamber", "B. Closing the door of the environmental chamber after placing it", "C. Placing it inside the environmental chamber", "D. Adjusting its position inside the environmental chamber"], "answer": "C", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57332/clip_11.mp4"}} |
| {"qid": "ExpVid_648", "question": "What material appears in this procedure?", "options": ["A. perlite", "B. peat moss", "C. substrate", "D. vermiculite"], "answer": "C", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58674/clip_36.mp4"}} |
| {"qid": "ExpVid_3340", "question": "What tool is being used by the researcher in this video clip?", "options": ["A. twist tie", "B. plant tag", "C. zip tie", "D. rubber band"], "answer": "A", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50527/clip_52.mp4"}} |
| {"qid": "ExpVid_2918", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. Data transfer cable", "B. USB cable", "C. Power cord", "D. Ethernet cable"], "answer": "B", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55979/clip_14.mp4"}} |
| {"qid": "ExpVid_44", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. aerosol seed particles", "B. seed crystals", "C. talcum powder", "D. silica nanoparticles"], "answer": "A", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55685/clip_55.mp4"}} |
| {"qid": "ExpVid_3269", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. cell spreader", "B. microscope slide", "C. agar plate", "D. culture dish"], "answer": "C", "subcategory": "research/environment", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61798/clip_27.mp4"}} |
| {"qid": "ExpVid_3283", "question": "What laboratory tool is being used in this research step?", "options": ["A. microscope slide", "B. cover slip", "C. well slide", "D. petri dish"], "answer": "B", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57774/clip_55.mp4"}} |
| {"qid": "ExpVid_2625", "question": "What temperature is set on the centrifuge?", "options": ["A. 4°C", "B. 0°C", "C. 25°C", "D. 37°C"], "answer": "A", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57021/clip_15.mp4"}} |
| {"qid": "ExpVid_2861", "question": "What volume is being pipetted per spot?", "options": ["A. 50 μL", "B. 100 μL", "C. 70 μL", "D. 90 μL"], "answer": "C", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58971/clip_14.mp4"}} |
| {"qid": "ExpVid_3889", "question": "What laboratory equipment is being utilized in this procedure?", "options": ["A. 1.5-milliliter PCR tube", "B. microcentrifuge tube rack", "C. Eppendorf tube", "D. 1.5-milliliter microcentrifuge tube"], "answer": "D", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61383/clip_20.mp4"}} |
| {"qid": "ExpVid_2657", "question": "What volume is being dispensed by the pipette?", "options": ["A. 21.0 µL", "B. 12.5 µL", "C. 21.5 µL", "D. 25.1 µL"], "answer": "C", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61124/clip_50.mp4"}} |
| {"qid": "ExpVid_4018", "question": "Which tool is being used in this experimental step?", "options": ["A. magnetic rack", "B. tube rack", "C. centrifuge", "D. vortex mixer"], "answer": "A", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61231/clip_28.mp4"}} |
| {"qid": "ExpVid_3583", "question": "Which scientific instrument is being employed in this experiment?", "options": ["A. pipette", "B. syringe", "C. dropper", "D. burette"], "answer": "A", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61124/clip_5.mp4"}} |
| {"qid": "ExpVid_330", "question": "Which material appears in this experimental step?", "options": ["A. residue", "B. precipitate", "C. aggregate", "D. pellet"], "answer": "D", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55463/clip_44.mp4"}} |
| {"qid": "ExpVid_3156", "question": "What laboratory tool is being used in this research step?", "options": ["A. Petri dish", "B. LB plate", "C. Microtiter plate", "D. Cell culture flask"], "answer": "B", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58267/clip_22.mp4"}} |
| {"qid": "ExpVid_744", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. sterile saline", "B. inoculates for 10 to the four dilution", "C. nutrient agar plates", "D. sterile dilution blanks"], "answer": "B", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/54752/clip_28.mp4"}} |
| {"qid": "ExpVid_1655", "question": "What is the person doing with the tube after adding liquids?", "options": ["A. Centrifuging the tube", "B. Vortexing the tube", "C. Hand-shaking the tube", "D. Inverting the tube to mix"], "answer": "B", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58267/clip_11.mp4"}} |
| {"qid": "ExpVid_1210", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. isopropanol", "B. ethanol", "C. PBS", "D. methanol"], "answer": "B", "subcategory": "research/genetics", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56423/clip_34.mp4"}} |
| {"qid": "ExpVid_325", "question": "What material appears in this procedure?", "options": ["A. diluted virus", "B. phosphate-buffered saline (PBS)", "C. fetal bovine serum (FBS)", "D. 0.9% saline solution"], "answer": "A", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/2137/clip_25.mp4"}} |
| {"qid": "ExpVid_437", "question": "Which material appears in this experimental step?", "options": ["A. 5% dextrose solution", "B. lactated Ringer's solution", "C. pre-warmed sterile 0.9% sodium chloride", "D. sterile water for injection"], "answer": "C", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/60599/clip_6.mp4"}} |
| {"qid": "ExpVid_2480", "question": "What volume of lipid-containing oil solution is being layered?", "options": ["A. 100 μL", "B. 15 μL", "C. 150 μL", "D. 200 μL"], "answer": "C", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/59555/clip_17.mp4"}} |
| {"qid": "ExpVid_1506", "question": "What is the person doing with the pipette tip in the microtiter plate?", "options": ["A. Transferring supernatant to a new plate", "B. Mixing the sample by pipetting up and down", "C. Drawing up intact cells from the bottom", "D. Dispensing liquid back into the original tube"], "answer": "A", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/51161/clip_20.mp4"}} |
| {"qid": "ExpVid_2228", "question": "What volume of medium is shown being added?", "options": ["A. 25 mL", "B. 15 mL", "C. 30 mL", "D. 20 mL"], "answer": "A", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/2693/clip_16.mp4"}} |
| {"qid": "ExpVid_94", "question": "Which material appears in this experimental step?", "options": ["A. caudate lobe", "B. left lobe", "C. right lobe", "D. medial lobe"], "answer": "B", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3642/clip_25.mp4"}} |
| {"qid": "ExpVid_3423", "question": "Which tool is being used in this experimental step?", "options": ["A. well plate", "B. beaker", "C. flask", "D. dish"], "answer": "D", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50196/clip_25.mp4"}} |
| {"qid": "ExpVid_716", "question": "Which material appears in this experimental step?", "options": ["A. cotyledons", "B. pre-infiltration whole leaves", "C. leaf between the veins 24 to 48 hours after infiltration", "D. stem cross-sections"], "answer": "C", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/55301/clip_29.mp4"}} |
| {"qid": "ExpVid_1909", "question": "What is the person doing with the pipette?", "options": ["A. Labeling sample tubes", "B. Adjusting the volume setting on the pipette", "C. Mixing the contents of a tube", "D. Dispensing liquid into a container"], "answer": "D", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58818/clip_26.mp4"}} |
| {"qid": "ExpVid_145", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. Triton X-100", "B. fixed cells", "C. nuclei", "D. cells"], "answer": "D", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/58083/clip_25.mp4"}} |
| {"qid": "ExpVid_808", "question": "Which material appears in this experimental step?", "options": ["A. LB broth", "B. nuclease-free water", "C. TE buffer", "D. negative resource material"], "answer": "D", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/53672/clip_40.mp4"}} |
| {"qid": "ExpVid_3694", "question": "Which tool is being utilized in this experimental technique?", "options": ["A. conical 50-milliliter tube", "B. centrifuge bottle", "C. test tube", "D. microcentrifuge tube"], "answer": "A", "subcategory": "research/immunology-infection", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56825/clip_19.mp4"}} |
| {"qid": "ExpVid_2372", "question": "What duration is shown on the timer during the initial adjustment phase?", "options": ["A. 20 minutes", "B. 10 minutes", "C. 30 minutes", "D. 15 minutes"], "answer": "D", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/201866/clip_37.mp4"}} |
| {"qid": "ExpVid_393", "question": "What material appears in this procedure?", "options": ["A. nitrogen gas", "B. 100% oxygen", "C. carbon dioxide", "D. nitrous oxide"], "answer": "B", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/51079/clip_34.mp4"}} |
| {"qid": "ExpVid_137", "question": "Which material appears in this experimental step?", "options": ["A. PSS alone", "B. acetylcholine solution", "C. lowest concentration of the experimental reagent diluted in PSS", "D. Krebs-Henseleit solution"], "answer": "C", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56639/clip_24.mp4"}} |
| {"qid": "ExpVid_1454", "question": "What is the person doing with the pachymetry probe?", "options": ["A. Shaking off excess fluid", "B. Rinsing it with saline solution", "C. Wiping it with sterile gauze", "D. Cleaning it with isopropanol"], "answer": "D", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3847/clip_15.mp4"}} |
| {"qid": "ExpVid_60", "question": "Which material appears in this experimental step?", "options": ["A. mucosa", "B. forceps", "C. cartilage", "D. tissue"], "answer": "D", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/59396/clip_10.mp4"}} |
| {"qid": "ExpVid_1626", "question": "What is the person doing with the scissors?", "options": ["A. Making a vertical abdominal incision from upper to lower torso", "B. Excising a rectangular tissue sample", "C. Creating a horizontal incision across the abdomen", "D. Cutting a short vertical clip near the umbilicus"], "answer": "A", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/201866/clip_8.mp4"}} |
| {"qid": "ExpVid_1902", "question": "What is the researcher doing with the colon after collection?", "options": ["A. Rinsing it gently with PBS", "B. Flushing it with PBS", "C. Injecting PBS into it", "D. Removing PBS from it"], "answer": "B", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50210/clip_8.mp4"}} |
| {"qid": "ExpVid_2287", "question": "What is the length of the skin incision?", "options": ["A. 1.5 cm", "B. 2 cm", "C. 1 cm", "D. 0.5 cm"], "answer": "C", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56679/clip_10.mp4"}} |
| {"qid": "ExpVid_1881", "question": "What is the surgeon doing with the surgical instrument near the heart vessel?", "options": ["A. Clamping the LADA with forceps", "B. Cauterizing the LADA tissue", "C. Tying a knot around the LADA", "D. Injecting fluid into the LADA"], "answer": "C", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/2464/clip_49.mp4"}} |
| {"qid": "ExpVid_1037", "question": "What material appears in this procedure?", "options": ["A. cells", "B. microcarrier beads", "C. collagen", "D. trypsin"], "answer": "A", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/50210/clip_22.mp4"}} |
| {"qid": "ExpVid_917", "question": "What material appears in this procedure?", "options": ["A. SYBR Green dye", "B. loading buffer", "C. DNA polymerase", "D. master mix"], "answer": "D", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3874/clip_35.mp4"}} |
| {"qid": "ExpVid_2692", "question": "What volume of growth media is being added?", "options": ["A. 5 mL", "B. 2 mL", "C. 1 mL", "D. 3 mL"], "answer": "B", "subcategory": "research/medicine", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/51513/clip_17.mp4"}} |
| {"qid": "ExpVid_824", "question": "Which material appears in this experimental step?", "options": ["A. glass coverslip", "B. embryo chorion", "C. embryo's abdomen", "D. agarose gel"], "answer": "C", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/57988/clip_4.mp4"}} |
| {"qid": "ExpVid_397", "question": "What material appears in this procedure?", "options": ["A. DMEM", "B. fresh medium A", "C. PBS", "D. trypsin solution"], "answer": "B", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/67016/clip_10.mp4"}} |
| {"qid": "ExpVid_3061", "question": "What equipment is being used in this scientific procedure?", "options": ["A. amplifier", "B. stimulator", "C. preamplifier", "D. oscilloscope"], "answer": "A", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61330/clip_25.mp4"}} |
| {"qid": "ExpVid_4005", "question": "What laboratory tool is being used in this research step?", "options": ["A. chamber slide", "B. microscope slide", "C. culture plate", "D. cover plate"], "answer": "D", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/56651/clip_40.mp4"}} |
| {"qid": "ExpVid_300", "question": "What material appears in this procedure?", "options": ["A. saline solution", "B. water", "C. phosphate-buffered saline (PBS)", "D. acetone"], "answer": "B", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/4142/clip_41.mp4"}} |
| {"qid": "ExpVid_2308", "question": "What volume of solution is added using the pipette?", "options": ["A. 100 μL", "B. 60 μL", "C. 40 μL", "D. 50 μL"], "answer": "D", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61330/clip_40.mp4"}} |
| {"qid": "ExpVid_2197", "question": "What is the person doing with the experimental sample?", "options": ["A. Storing it in a refrigerator", "B. Labeling it with a marker", "C. Covering it with aluminum foil", "D. Placing it in a light-proof container"], "answer": "D", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/52299/clip_51.mp4"}} |
| {"qid": "ExpVid_481", "question": "What material appears in this procedure?", "options": ["A. head coil", "B. subject", "C. calibration mannequin", "D. MRI phantom"], "answer": "B", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/201832/clip_19.mp4"}} |
| {"qid": "ExpVid_2093", "question": "What is the person doing with the forceps?", "options": ["A. Cleaning the electrode tips with solution", "B. Opening the electrode in the bath", "C. Adjusting the electrode position in the bath", "D. Closing the electrode after immersion"], "answer": "B", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/63838/clip_41.mp4"}} |
| {"qid": "ExpVid_212", "question": "What material appears in the researcher's work in this video clip?", "options": ["A. restriction enzyme", "B. fluorescent antibody", "C. reporter gene", "D. expression plasmid"], "answer": "C", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/62650/clip_3.mp4"}} |
| {"qid": "ExpVid_3309", "question": "Which scientific instrument is being employed in this experiment?", "options": ["A. oven", "B. water bath", "C. incubator", "D. refrigerator"], "answer": "C", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/3691/clip_41.mp4"}} |
| {"qid": "ExpVid_209", "question": "Which material appears in this experimental step?", "options": ["A. dura mater", "B. calvarium", "C. periosteum", "D. scalp"], "answer": "D", "subcategory": "research/neuroscience", "video_ref": {"repo": "OpenGVLab/ExpVid", "zip_path": "", "member": "videos/level_1/61330/clip_6.mp4"}} |
|
|
